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Anti-KCNH5 (EAG-2) Antibody

KV10.2, Ether-a-go-go potassium channel 2, hEAG2

Cat #: APC-053
Alternative Name KV10.2, Ether-a-go-go potassium channel 2, hEAG2
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, r
    Immunogen
    • Peptide (C)EDPKGSDSENSVTKNPLRK, corresponding to amino acid residues 842-860 of rat KCNH5 (Accession Q9EPI9). Intracellular, C-terminus.
    Accession (Uniprot) Number Q9EPI9
    Gene ID 171146
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human - 18/19 amino acid residues identical.
    RRID AB_2039935.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Specificity The antibody is specific for KV10.2 and will not cross-react with KV10.1.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Western blot analysis of rat brain membranes:
      Western blot analysis of rat brain membranes:
      1. Anti-KCNH5 (EAG-2) Antibody (#APC-053), (1:200).   
      2. Anti-KCNH5 (EAG-2) Antibody, preincubated with KCNH5/EAG-2 Blocking Peptide (#BLP-PC053).
    Immunohistochemistry
    References
    1. Ludwig, J. et al. (2000) Mol. Sci. Neurosci. 16, 59.
    2. Ju, M. et al. (2002) FEBS Lett. 524, 204.
    Scientific background

    KV10.2 is a member of the Ether-a-go-go family of voltage-activated potassium channels. To date, the family includes eight members: the closely related KV10.1 protein, the KV12 (elk) (elk1, elk2, elk3) and the KV11 (erg) proteins (erg1, erg2, erg3).  

    The eag subfamily current is characterized by outward rectification without inactivation and slow activation kinetics. No specific pharmacological blocker has been described.

    Tissue distribution of KV10.2 expression in rats is restricted to neuronal tissue with greater expression in the cortex and thalamus. In humans, KV10.2 mRNA was detected in heart and skeletal muscle in addition to brain. The physiological role of KV10.2 is currently unknown.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2021

    Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat KV10.2 channel. Anti-KCNH5 (EAG-2) Antibody (#APC-053) can be used for western blot and immunohistochemistry applications. It has been designed to recognize KV10.2 from rat and human samples. The antibody is specific for KV10.2 and will not cross-react with KV10.1.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Western blot and immunocytochemistry of rat hippocampus. Tested in Kv10.2 knockout rats.
      Liu, Y. et al. (2020) Neurochem. Res. 45, 2949.
    More product citations
    1. Wadhwa, S. et al. (2009) Int. Urol. Nephrol. 41, 251.
    2. Bracey, K. et al. (2008) J. Membr. Biol. 222, 115.
    3. Jow, G.M. and Jeng, C.J. (2008) Neurosci. Lett. 431, 12.
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