Anti-KV3.2 (KCNC2) Antibody

Potassium voltage-gated channel subfamily C member 2, Shaw-like potassium channel, KSHIIIA
    Cat #: APC-011
    Alternative Name Potassium voltage-gated channel subfamily C member 2, Shaw-like potassium channel, KSHIIIA
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide DLGGKRLGIEDAAGLGGPDGK(C), corresponding to amino acid residues 184-204 of rat KV3.2 (Accession P22462). Intracellular, N-terminal part.
        Anti-KV3.2 (KCNC2) Antibody
    Accession (Uniprot) Number P22462
    Gene ID 246153
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human - 19/21 amino acid residues identical (based on ESTs).
    RRID AB_2040168.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.3 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, ip, wb
    May also work in: ic*, ifc*
      • Anti-KV3.2 (KCNC2) Antibody
        Western blot analysis of rat brain membranes:
        1. Anti-KV3.2 (KCNC2) Antibody (#APC-011), (1:200).
        2. Anti-KV3.2 (KCNC2) Antibody, preincubated with the negative control antigen.
        Mouse sciatic nerve lysate (Sobko, A. et al. (1998) J. Neurosci. 18, 10398.).
      • CHO transfected cells (Lewis, A. et al. (2004) J. Biol. Chem. 279, 7884.).
      • Rat brain sections. Human pancreatic islets (Yan, L. et al. (2004) Diabetes 53, 597.).
    References
    • 1. Rettig, J. et al. (1992) EMBO J. 11, 2473.
    • 2. Rudy, B. et al. (1999) Ann. NY Acad. Sci. 868, 304.
    • 3. Rudy, B. et al. (2001) Trends Neurosci. 24, 517.
      • KV3.2 is a member of the voltage-gated K+ channel superfamily. Together with the related proteins KV3.1, KV3.3 and KV3.4 they constitute the Shaw type subfamily family.1

        As with all KV channels, KV3.2 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C-termini. The functional KV channel is a tetramer that can either be a homomer or a heteromer of KV3 subunits.

        KV3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing.2,3

        KV3.2 is highly expressed in the brain but has been also detected in peripheral organs such as pancreas and mesenteric artery.

        KV3.2 and KV3.1 are highly enriched in neurons that fire at high frequencies, such as fast-spiking interneurons of the cortex and hippocampus and neurons in the globus pallidus. Their unusually rapid activation and deactivation rates allow channels containing KV3.2 and KV3.1 subunits to repolarize action potentials quickly thus minimizing the rate of recovery of sodium channel inactivation.2,3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KV3.2. Anti-KV3.2 (KCNC2) Antibody (#APC-011) can be used in western blot, immunoprecipitation, and immunohistochemistry applications. It has been designed to recognize KV3.2 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Rat lumbar spinal cord sections.
        Wolff, M. et al. (2016) Neurosci. Res. 109, 16.
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