Anti-KCNC4 (KV3.4) Antibody

Potassium voltage-gated channel subfamily C member 4, Raw3, KSHIIIC
Cat #: APC-019
Alternative Name Potassium voltage-gated channel subfamily C member 4, Raw3, KSHIIIC
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide EAGDD ERELA LQRLG PHEG(C), corresponding to amino acid residues 177-195 of rat KCNC4 (Accession Q63734). Intracellular, N-terminal.
    Accession (Uniprot) Number Q63734
    Gene ID 684516
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human - identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.3 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, ip, wb
    May also work in: ifc
      • Western blot analysis of rat brain membranes:
        1. Anti-KCNC4 (KV3.4) Antibody (#APC-019), (1:200).
        2. Anti-KCNC4 (KV3.4) Antibody, preincubated with the control peptide antigen.
      • Transfected COS-7 cells (10 μg antibody with 1.5 mg total protein) (Abbott, G.W. et al. (2001) Cell 104, 217.).
      • Rat brain sections.

        Mouse transversus abdominus (1:100-1:1000) (Brooke, R.E. et al. (2004) Eur. J. Neurosci20, 3313.).
      • Rabbit carotid body (1:100) (Kaab, S. et al. (2004) J. Physiol566, 395.).
      • KV3.4 is a member of the voltage-gated K+ channel superfamily. Together with the related proteins KV3.1, KV3.2 and KV3.3 they constitute the Shaw type subfamily family.1

        As with all Kv channels, KV3.4 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional KV channel is a tetramer that can either be a homomer or a heteromer of KV3 subunits.2

        Kv3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing.2

        Kv3.4 expression is wide and the channel can be found in brain, skeletal muscle, prostate and pancreas among others.

        KV3.4 subunits have been implicated recently in the response mechanism to chronic hypoxia and the etiology of Alzheimer's and Parkinson's diseases. In addition, KV3.4 was found to associate with the auxiliary subunit KCNE3 (MirP2) in skeletal muscle. A mutation in KCNE3 (R83H) has been associated with an inherited form of periodic paralysis (Thyrotoxic hypokalemic periodic paralysis) that is caused by the altered physiological function of the KV3.4 channel.3

        Toxins from sea anemone, BDS-I (#STB-400), (47 nM) and BDS-II (#B-450), (56 nM), are potent blockers of KV3.4 channels.4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-KCNC4 (Kv3.4) Antibody
    Expression of KV3.4 in rat dorsal horn.Immunohistochemical staining of rat spinal cord sections using Anti-KCNC4 (KV3.4) Antibody (#APC-019). KV3.4 staining (green) is observed in dorsal horn laminae I–III. KV3.4 immunoreactivity partially co-localizes with CGRP (red).Adapted from Muqeem, T. et al. (2018) J. Neurosci. 38, 3729. with permission of the Society for Neuroscience.

    Last update: 08/04/2019

    Anti-KCNC4 (KV3.4) Antibody (#APC-019) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KV3.4 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Decrease in KV3.4 cell surface expression following SCI.
        Immunohistochemical staining of rat dorsal root ganglion following laminectomy (upper panels) and spinal cord injury (SCI) (lower panels) using Anti-KCNC4 (KV3.4) Antibody (#APC-019). KV3.4 surface expression (green) decreases following SCI as opposed to laminectomy.
        Adaped from Ritter, D.M. et al. (2015) with permission of the Society for Neuroscience.
      • Transfected COS-7 cells (10 μg antibody with 1.5 mg total protein).
        Abbott, G.W. et al. (2001) Cell 104, 217.
      • Rat brain sections.
        Muqeem, T. et al. (2018) J. Neurosci. 38, 3729.

      • Rat lumbar spinal cord sections.
        Wolff, M. et al. (2016) Neurosci. Res. 109, 16.
      • Rat DRG sections (1:1000).
        Ritter, D.M. et al. (2015) J. Neurosci. 35, 1260.
      • Mouse transversus abdominus (1:100-1:1000).
        Brooke, R.E. et al. (2004) Eur. J. Neurosci. 20, 3313.
      • Rabbit carotid body (1:100).
        Kaab, S. et al. (2004) J. Physiol. 566, 395.
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