Free shipping starts now, no minimum, no coupons required!

Anti-KCNC4 (KV3.4) Antibody

Potassium voltage-gated channel subfamily C member 4, Raw3, KSHIIIC

Cat #: APC-019
Alternative Name Potassium voltage-gated channel subfamily C member 4, Raw3, KSHIIIC
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
  • Peptide EAGDD ERELA LQRLG PHEG(C), corresponding to amino acid residues 177-195 of rat KCNC4 (Accession Q63734). Intracellular, N-terminal.
Accession (Uniprot) Number Q63734
Gene ID 684516
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human - identical.
RRID AB_2040172.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.3 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, ip, wb
May also work in: ifc*
Western blot
  • Western blot analysis of rat brain lysates:
    Western blot analysis of rat brain lysates:
    1. Anti-KCNC4 (KV3.4) Antibody (#APC-019), (1:200).
    2. Anti-KCNC4 (KV3.4) Antibody, preincubated with KCNC4/Kv3.4 Blocking Peptide (#BLP-PC019).
  • Transfected COS-7 cells (10 μg antibody with 1.5 mg total protein) (Abbott, G.W. et al. (2001) Cell 104, 217.).
  • Rat brain sections.

    Mouse transversus abdominus (1:100-1:1000) (Brooke, R.E. et al. (2004) Eur. J. Neurosci20, 3313.).
  • Rabbit carotid body (1:100) (Kaab, S. et al. (2004) J. Physiol566, 395.).
  1. Schroter, K.H. et al. (1991) FEBS Lett. 278, 211.
  2. Rettig, J. et al. (1992) EMBO J. 11, 2473.
  3. Abbott, G.W. et al. (2001) Cell 104, 217.
  4. Diochot, S. et al. (1998) J. Biol. Chem. 273, 6744.
Scientific background

KV3.4 is a member of the voltage-gated K+ channel superfamily. Together with the related proteins KV3.1, KV3.2 and KV3.3 they constitute the Shaw type subfamily family.1

As with all Kv channels, KV3.4 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional KV channel is a tetramer that can either be a homomer or a heteromer of KV3 subunits.2

Kv3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing.2

Kv3.4 expression is wide and the channel can be found in brain, skeletal muscle, prostate and pancreas among others.

KV3.4 subunits have been implicated recently in the response mechanism to chronic hypoxia and the etiology of Alzheimer's and Parkinson's diseases. In addition, KV3.4 was found to associate with the auxiliary subunit KCNE3 (MirP2) in skeletal muscle. A mutation in KCNE3 (R83H) has been associated with an inherited form of periodic paralysis (Thyrotoxic hypokalemic periodic paralysis) that is caused by the altered physiological function of the KV3.4 channel.3

Toxins from sea anemone, BDS-I (#STB-400), (47 nM) and BDS-II (#B-450), (56 nM), are potent blockers of KV3.4 channels.4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-KCNC4 (Kv3.4) Antibody
Expression of KV3.4 in rat dorsal horn.Immunohistochemical staining of rat spinal cord sections using Anti-KCNC4 (KV3.4) Antibody (#APC-019). KV3.4 staining (green) is observed in dorsal horn laminae I–III. KV3.4 immunoreactivity partially co-localizes with CGRP (red).Adapted from Muqeem, T. et al. (2018) J. Neurosci. 38, 3729. with permission of the Society for Neuroscience.

Last update: 22/05/2022

Anti-KCNC4 (KV3.4) Antibody (#APC-019) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KV3.4 from human, rat, and mouse samples.

For research purposes only, not for human use



Published figures using this product
  • Decrease in KV3.4 cell surface expression following SCI.
    Decrease in KV3.4 cell surface expression following SCI.
    Immunohistochemical staining of rat dorsal root ganglion following laminectomy (upper panels) and spinal cord injury (SCI) (lower panels) using Anti-KCNC4 (KV3.4) Antibody (#APC-019). KV3.4 surface expression (green) decreases following SCI as opposed to laminectomy.
    Adaped from Ritter, D.M. et al. (2015) with permission of the Society for Neuroscience.
Immunoprecipitation citations
  1. Transfected COS-7 cells (10 μg antibody with 1.5 mg total protein).
    Abbott, G.W. et al. (2001) Cell 104, 217.
Immunohistochemistry citations
  1. Rat brain sections.
    Muqeem, T. et al. (2018) J. Neurosci. 38, 3729.
  2. Rat lumbar spinal cord sections.
    Wolff, M. et al. (2016) Neurosci. Res. 109, 16.
  3. Rat DRG sections (1:1000).
    Ritter, D.M. et al. (2015) J. Neurosci. 35, 1260.
  4. Mouse transversus abdominus (1:100-1:1000).
    Brooke, R.E. et al. (2004) Eur. J. Neurosci. 20, 3313.
Immunocytochemistry citations
  1. Rabbit carotid body (1:100).
    Kaab, S. et al. (2004) J. Physiol. 566, 395.
More product citations
  1. Gopel, S.O. et al. (2000) J. Physiol. 528.3, 497.


Scientific Background

Shipping and Ordering information