This product is freeze dried. All water molecules have been removed.
This antibody is shipped with its antigen FREE of charge!
This lyophilized product is shipped at room temperature. Please see its certificate of analysis for further storage instructions.
2. Anti-KCNC4 (KV3.4) Antibody, preincubated with the control peptide antigen.
Mouse transversus abdominus (1:100-1:1000) (Brooke, R.E. et al. (2004) Eur. J. Neurosci. 20, 3313.).
KV3.4 is a member of the voltage-gated K+ channel superfamily. Together with the related proteins KV3.1, KV3.2 and KV3.3 they constitute the Shaw type subfamily family.1
As with all Kv channels, KV3.4 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional KV channel is a tetramer that can either be a homomer or a heteromer of KV3 subunits.2
Kv3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing.2
Kv3.4 expression is wide and the channel can be found in brain, skeletal muscle, prostate and pancreas among others.
KV3.4 subunits have been implicated recently in the response mechanism to chronic hypoxia and the etiology of Alzheimer's and Parkinson's diseases. In addition, KV3.4 was found to associate with the auxiliary subunit KCNE3 (MirP2) in skeletal muscle. A mutation in KCNE3 (R83H) has been associated with an inherited form of periodic paralysis (Thyrotoxic hypokalemic periodic paralysis) that is caused by the altered physiological function of the KV3.4 channel.3
Species reactivity key:
Expression of KV3.4 in rat dorsal horn.Immunohistochemical staining of rat spinal cord sections using Anti-KCNC4 (KV3.4) Antibody (#APC-019). KV3.4 staining (green) is observed in dorsal horn laminae I–III. KV3.4 immunoreactivity partially co-localizes with CGRP (red).Adapted from Muqeem, T. et al. (2018) J. Neurosci. 38, 3729. with permission of the Society for Neuroscience.
Anti-KCNC4 (KV3.4) Antibody (#APC-019) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KV3.4 from human, rat, and mouse samples.
Adaped from Ritter, D.M. et al. (2015) with permission of the Society for Neuroscience.
- Transfected COS-7 cells (10 μg antibody with 1.5 mg total protein).
Abbott, G.W. et al. (2001) Cell 104, 217.
Rat brain sections.
Muqeem, T. et al. (2018) J. Neurosci. 38, 3729.
- Rat lumbar spinal cord sections.
Wolff, M. et al. (2016) Neurosci. Res. 109, 16.
- Rat DRG sections (1:1000).
Ritter, D.M. et al. (2015) J. Neurosci. 35, 1260.
- Mouse transversus abdominus (1:100-1:1000).
Brooke, R.E. et al. (2004) Eur. J. Neurosci. 20, 3313.
- Rabbit carotid body (1:100).
Kaab, S. et al. (2004) J. Physiol. 566, 395.
- Gopel, S.O. et al. (2000) J. Physiol. 528.3, 497.