Anti-KV3.1b (KCNC1) Antibody

Potassium voltage-gated channel subfamily C member 1, NGK2, RAW2
    Cat #: APC-014
    Alternative Name Potassium voltage-gated channel subfamily C member 1, NGK2, RAW2
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide CKESPVIAKYMPTEAVRVT, corresponding to amino acid residues 567-585 of rat KV3.1b (Accession P25122). Intracellular, C-terminus.
        Anti-KV3.1b (KCNC1) Antibody
    Accession (Uniprot) Number P25122
    Gene ID 25327
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human, dog – identical.
    RRID AB_2040166.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
      • Anti-KV3.1b (KCNC1) Antibody
        Western blot analysis of rat brain membranes:
        1. Anti-KV3.1b (KCNC1) Antibody (#APC-014), (1:200).
        2. Anti-KV3.1b (KCNC1) Antibody, preincubated with the control peptide antigen.
      • Transfected COS-7 cells (Wu, H. et al. (1998) J. Cell Sci. 111, 2365.).
      • Rat brain sections. Mouse medial nucleus of the trapezoid body (MNTB) (1:500) (Leao, K.E. et al. (2010) Eur. J. Neurosci. 32, 1658.).
      • Rat neural progenitor cells (NPCs) (1:200) (Liebau, S. et al. (2006) J. Neurochem99, 426.).
    References
      • KV3.1 is a member of the voltage-gated K+ channel superfamily. Together with the related proteins KV3.2KV3.3 and KV3.4 they constitute the Shaw type subfamily family.1

        As with all KV channels, KV3.1 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional Kv channel is a tetramer that can either be a homomer or a heteromer of KV3 subunits.

        KV3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of  action potentials and to facilitate repetitive high frequency firing.3

        KV3.1 is highly expressed in the brain but has been also detected in peripheral organs such as lung skeletal muscle and testis.

        KV3.1 and KV3.2 are highly enriched in neurons that fire at high frequencies, such as fast spiking interneurons of the cortex and hippocampus and neurons in the globus pallidus. Their unusually rapid activation and deactivation rates allow channels containing KV3.1 and KV3.2 subunits to repolarize action potentials quickly thus minimizing the rate of recovery of sodium channel inactivation.3

        Lack of KV3.1 channel subunits is mainly responsible for constitutively increased locomotor activity and sleep loss.2

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-Kv3.1b (KCNC1) Antibody
    Knockout validation of Anti-KV3.1b (KCNC1) Antibody in mouse brain.Western blot analysis of mouse brain lysates using Anti-KV3.1b (KCNC1) Antibody (#APC-014). KV3.1b immunodetection is abolished in KV3.1-/- mice. β-tubulin is used as a loading control.Adapted from Jukkola, P. et al. (2017) Front. Mol. Neurosci. 10, 344.

    Last update: 24/01/2020

    Anti-KV3.1b (KCNC1) Antibody (#APC-014) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation applications. It has been designed to recognize KV3.1b from rat, mouse, and human samples.

    For research purposes only, not for human use
    Citations
      • Anti-KV3.1b (KCNC1) Antibody
        Co-localization of KV3.1b and KIF5 in rat hippocampal neurons
        Immunocytochemical staining of rat hippocampal neurons with Anti-KV3.1b (KCNC1) Antibody (APC-014) and anti-KIF5 antibody. Arrows in right panel depict colocalization between the two proteins.
        Adapted from Barry, J. et al. (2013) with permission of The Company of Biologists LTD.
      • Western blot analysis of mouse brain lysates. Also tested in KV3.1-/- mice.
        Jukkola, P. et al. (2017) Front. Mol. Neurosci. 10, 344.
      • Western blot analysis of mouse brain lysates. Immunohistochemical staining of mouse coronal cerebellum sections. Immunocytochemical staining of mouse cultured hippocampal and cerebellar neurons. Tested in KV3.1b knockout mice and KV3.1b knockout mice cultured cerebellar neurons.
        Barry, J. et al. (2013) J. Cell Sci. 126, 2027.
      • Mouse brain lysates. Also tested in KV3.1-/- mice.
        Jukkola, P. et al. (2017) Front. Mol. Neurosci. 10, 344.
      • Rat lung lysate.
        Lv, Y. et al. (2013) Am. J. Physiol. 305, L856.
      • Mouse brain lysates. Also tested in KV3.1b knockout mice.
        Barry, J. et al. (2013) J. Cell Sci. 126, 2027.
      • Transfected COS-7 cells.
        Wu, H. et al. (1998) J. Cell Sci. 111, 2365.
      • Rat lumbar spinal cord sections.
        Wolff, M. et al. (2016) Neurosci. Res. 109, 16.
      • Mouse brain sections (1:800).
        Blosa, M. et al. (2015) J. Physiol. 593, 4341.
      • Mouse coronal cerebellum sections. Also tested in same sections from KV3.1b knockout mice.
        Barry, J. et al. (2013) J. Cell Sci. 126, 2027.
      • Mouse medial nucleus of the trapezoid body (MNTB) (1:500).
        Leao, K.E. et al. (2010) Eur. J. Neurosci. 32, 1658.
      • Mouse cultured hippocampal neurons and mouse cultured cerebellar neurons. Also tested in KV3.1b knockout mice cultured cerebellar neurons.
        Barry, J. et al. (2013) J. Cell Sci. 126, 2027.
      • Rat neural progenitor cells (NPCs) (1:200).
        Liebau, S. et al. (2006) J. Neurochem. 99, 426.
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