Anti-LPAR1 (EDG2) (extracellular) Antibody

Lysophosphatidic acid receptor 1, LPA receptor 1, LPA-1, Gpcr91, Gpr26, VZG1
    Cat #: ALR-031
    Alternative Name Lysophosphatidic acid receptor 1, LPA receptor 1, LPA-1, Gpcr91, Gpr26, VZG1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)RSGKYLATEWNTVSK, corresponding to amino acid residues 36-50 of rat LPA1 receptor (Accession P61794). Extracellular, N-terminus.
    • Anti-LPAR1 (EDG2) (extracellular) Antibody
    Accession (Uniprot) Number P6179
    Gene ID 116744
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical; human – 14/15 amino acid residues identical.
    RRID AB_2756660.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*, lci*
    Western blot
    • Anti-LPAR1 (EDG2) (extracellular) Antibody
      Western blot analysis of rat brain membrane (lane 1 and 3), mouse brain membrane (lanes 2 and 4) and human brain glioblastoma U87 MG cell line (lanes 5 and 6) lysate: 
      1,2,5. Anti-LPAR1 (EDG2) (extracellular) Antibody (#ALR-031), (1:400).
      3,4,6. Anti-LPAR1 (EDG2) (extracellular) Antibody, preincubated with the negative control antigen.
    References
    1. Contos, J.J.A. et al. (2000) Mol. Pharmacol. 58, 1188.
    2. Gohla, A. et al. (1998) J. Biol. Chem. 273, 4653.
    3. Boucharaba, A. et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 9643.
    Scientific background

    Lysophosphatidic acid is a naturally occurring lysophospholipid that activates diverse cellular actions in various cells including proliferation and morphological changes. There are currently three known genes that encode proteins of the LPA receptor family- Edg2, Edg4 and Edg7.

    Lysophosphatidic acid receptor 1 (also known as Lpa1), encoded by Edg2, belongs to G-coupled protein receptor (GPCR) superfamily. All three proteins share 50-54% identical amino-acids. Lpa1 mostly activates the Gi pathway that leads to inhibition of adenylate cyclase and thus inhibition of cAMP production. Through this pathway, Lpa1 also initiates the Ras/MAP kinase cascade which is primarily responsible for increased cell proliferation1. Interestingly, binding of lysophosphatidic acid to the G13 G-protein activates the small GTP-binding protein Rho and induces the rapid remodeling of the actin cytoskeleton2.

    Lpa1 has been implicated in the pathogenesis of metastatic bone cancer. Silencing of Lpa1 expression in breast and ovarian cancer cells significantly alters the progression of bone metastasis through reduction of tumor burden and inhibition of bone resorption. A specific Lpa1 antagonist exhibits specificity for tumor cell-platelet interaction without abrogating normal platelet function3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-LPAR1 (EDG2) (extracellular) Antibody (#ALR-031) is a highly specific antibody directed against an epitope of the rat LPA1 receptor. The antibody can be used in western blot analysis. It has been designed against an extracellular domain and thus is highly suitable for detecting the receptor in living cells. It has been designed to recognize LPA1 receptor from human, rat, and mouse samples.

    For research purposes only, not for human use
    Shipping and Ordering information