Anti-SCN2A (NaV1.2) Antibody

BII, Brain type II Na+ channel, Sodium channel protein type 2 subunit alpha
    Cat #: ASC-002
    Alternative Name BII, Brain type II Na+ channel, Sodium channel protein type 2 subunit alpha
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775). Intracellular loop between domains I and II.
    • Anti-SCN2A (NaV1.2) Antibody
    Accession (Uniprot) Number P04775
    Gene ID 24766
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human - identical.
    NaV1.3 - 12/15 amino acid residues identical.
    RRID AB_2040005.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
    Western blot
    • Anti-SCN2A (NaV1.2) Antibody
      Western blot analysis of rat brain membranes:
      1. Anti-SCN2A (NaV1.2) Antibody (#ASC-002), (1:200).
      2. Anti-SCN2A (NaV1.2) Antibody, preincubated with the negative control antigen.
    Immunoprecipitation
    • Anti-SCN2A (NaV1.2) Antibody
      Immunoprecipitation of rat brain lysate:
      1. Rat brain lysates
      2. Lysates immunoprecipitated with Anti-SCN2A (NaV1.2) Antibody (#ASC-002), (6 µg).
      3. Lysates immunoprecipitated with pre-immune rabbit serum.
      The lower arrow indicates the IgG heavy chain.
      Western blot analysis was performed with Anti-SCN2A (NaV1.2) Antibody.
    Immunohistochemistry
    • Anti-SCN2A (NaV1.2) Antibody
      Expression of NaV1.2 in mouse hippocampus
      Immunohistochemical staining of mouse hippocampus using Anti-SCN2A (NaV1.2) Antibody (#ASC-002 ). A. NaV1.2 (red) is present in dendrites of pyramidal neurons in the CA3 region. B. Staining of interneurons in the pyramidal layer with mouse anti-Parvalbumin (green) demonstrates the restriction of NaV1.2 to dendrites (arrows) extending from the pyramidal layer (P). C. Confocal merge of panels A and B.
    Immunocytochemistry
    • Human NaV1.2 transfected in HEK-293 cells (1:30) (Kamiya, K. et al. (2004) J. Neurosci. 24, 2690.).
    References
    1. Wu, L. et al. (2002) NeuroReport 13, 2547.
    2. Fang, X. et al. (2002) J. Neurosci. 22, 7425.
    3. Fjell, J. et al. (2000) NeuroReport 11, 199.
    4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
    5. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
    6. Isom, L.L. (2001) Neuroscientist 7, 42.
    7. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
    8. Catterall, W.A. et al. (2008) J. Neurosci. 28, 11768.
    Scientific background

    Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3

    Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7

    NaV1.2, also known as SCN2A, is primarily expressed in the central nervous system (CNS) and is localized in unmyelinated or premyelinated axons and dendrites. Mutations in the NaV1.2 channel have been identified in different types of epilepsy.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-SCN2A (Nav1.2) Antibody NaV1.1 Levels Decrease in PV Cells of hAPP Mice and in AD Brains.A and B. Western blot analysis of parietal cortex from mice and inferior parietal cortex from humans using Anti-SCN1A (NaV1.1) Antibody (#ASC-001), Anti-SCN2A (NaV1.2) Antibody (#ASC-002), Anti-SCN3A (NaV1.3) Antibody (#ASC-004) and Anti-NaV1.6 (SCN8A) Antibody (#ASC-009). The graphs represent the quantitation of each western blot.Adapted from Verret, L. et al. (2012) with permission of Elsevier.
    Last update: 24/01/2020

    Anti-SCN2A (NaV1.2) Antibody (#ASC-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.2 from rat, human, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Published figures using this product
    • Anti-SCN2A (NaV1.2) Antibody
      Expression of NaV1.6 increases in rat mECs during epileptogenesis.
      A. Immunohistochemical of rat mECs using Anti-NaV1.6 (SCN8A) Antibody (#ASC-009). NaV1.6 staining is detected in AIS and increases in post-SE tissue. The channel co-localizes with Ankryn-G, a marker of AIS. B. Ratio of post-SE and control tissues shows that NaV1.6 expression increases by 46%. C. Somatal expression for NaV1.6 and NaV1.2 (using Anti-SCN2A (NaV1.2) Antibody (#ASC-002)). D. Normalized expression of the channel expression shows that both NaV1.2 and NaV1.6 expression increases in the soma of post-SE tissues. NaV1.1 and NaV1.3 expression, detected using Anti-SCN1A (NaV1.1) Antibody (#ASC-001) and Anti-SCN3A (NaV1.3) Antibody (#ASC-004), respectively, does not change during epileptogenesis.
      Adapted from Hargus, N.J. et al. (2013) with permission of the American Physiological Society.
    KO validation citations
    1. Immunohistochemical staining of mouse brain sections. Tested in SCN2A-/- mice.
      Planells-Cases, R. et al. (2000) Biophys. J. 78, 2878.
    Western blot citations
    1. Human astrocyte lysate.
      Guan, G. et al. (2018) Neurosci. Lett. 674, 148.
    2. Rat brain lysate.
      Murenzi, E. et al. (2017) Neurotoxicology 60, 260.
    3. Mouse brain lysate.
      Cesca, F. et al. (2015) J. Biol. Chem. 290, 18045.
    4. Mouse brain lysates.
      Liu, C. et al. (2015) J. Biol. Chem. 290, 12048.
    5. Rat pituitary (GH3) cell lysate.
      Baroni, D. et al. (2014) Biol. Cell 106, 13.
    6. Rat DRG lysate (1:200).
      Cheng, K.I. et al. (2014) Eur. J. Pain 18, 162.
    7. Human and mouse brain.
      Verret, L. et al. (2012) Cell 149, 708.
    8. Mouse heart.
      Haufe, V. et al. (2005) J. Physiol. 564, 683.
    9. Human brain.
      Kamiya, K. et al. (2004) J. Neurosci. 24, 2690.
    10. Rat brain.
      Malhotra, J.D. et al. (2000) J. Biol. Chem. 275, 11383.
    11. Mouse brain.
      Planells-Cases, R. et al. (2000) Biophys. J. 78, 2878.
    Immunoprecipitation citations
    1. Mouse brain lysate.
      Cesca, F. et al. (2015) J. Biol. Chem. 290, 18045.
    2. Mouse heart.
      Haufe, V. et al. (2005) J. Physiol. 564, 683.
    Immunohistochemistry citations
    1. Mouse brain sections.
      Yamagata, T. et al. (2017) Biochem. Biophys. Res. Commun. 491, 1070.
    2. Mouse brain sections (1:100).
      Katz, E. et al. (2018) Proc. Natl. Acad. Sci. U.S.A. 115, E7184.
    3. Rat lumbar spinal cord sections.
      Wolff, M. et al. (2016) Neurosci. Res. 109, 16.
    4. Mouse L5 pyramidal sections.
      Hamada, M.S. and Kole, M.H. (2015) J. Neurosci. 35, 7272.
    5. Rat optic nerve.
      Sandalon, S. et al. (2013) Exp. Eye Res. 115, 47.
    6. Rat brain sections (1:500).
      Qiao, X. et al. (2013) Epilepsy Res. 106, 17.
    7. Rat brain sections (1:250).
      Hargus, N.J. et al. (2013) J. Neurophysiol. 110, 1144.
    8. Rat brain.
      Hu, W. et al. (2009) Nat. Neurosci. 12, 996.
    9. Mouse heart.
      Lei, M. et al. (2004) J.Physiol. 559.3, 835.
    10. Guinea pig intestine.
      Hanani, M. et al. (2000) Am J. Physiol. Gastrointest Liver Physiol. 278, G644.
    11. Mouse brain sections. Also tested in SCN2A-/- mice.
      Planells-Cases, R. et al. (2000) Biophys. J. 78, 2878.
    Immunocytochemistry citations
    1. Rat microglia (1:400).
      Jung, G.Y. et al. (2013) Glia 61, 1807.
    2. Mouse heart.
      Haufe, V. et al. (2005) J. Physiol. 564, 683.
    3. Human brain.
      Kamiya, K. et al. (2004) J. Neurosci. 24, 2690.
    4. Mouse heart.
      Lei, M. et al. (2004) J.Physiol. 559.3, 835.
    5. Mouse brain.
      Planells-Cases, R. et al. (2000) Biophys. J. 78, 2878.
    6. Mouse brain.
      Zhou, D. et al. (1998) J. Cell Biol. 143, 1295.
    More product citations
    1. Tian, C. et al. (2009) Protocol Exchange doi:10.1038/nprot.2009.161.
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