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Guinea pig Anti-NaV1.7 (SCN9A) Antibody

Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA
Cat #: AGP-057
Alternative Name Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea pig
Reactivity: r
May also work in: h*, m*
Immunogen
  • Peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562). Intracellular loop between domains I and II.
Accession (Uniprot) Number O08562
Gene ID 78956
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, mouse - 13/15 amino acid residues identical.
RRID AB_2756613.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat DRG lysates:
    Western blot analysis of rat DRG lysates:

    1. Guinea pig Anti-NaV1.7 (SCN9A) Antibody (#AGP-057), (1:400).
    2. Guinea pig Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
     
    Following a broad screen of secondary antibodies, the following was used for this application:
    Western blot analysis:
    #106-035-006 (Jackson ImmunoResearch).
    Note: addition of 0.5% Tween-20 to the primary antibody solution may strengthen the signal and lower background.

References
  1. Wu, L. et al. (2002) NeuroReport 13, 2547.
  2. Fang, X. et al. (2002) J. Neurosci. 22, 7425.
  3. Fjell, J. et al. (2000) NeuroReport 11, 199.
  4. Baker, M.D. et al. (2001) Trends. Pharmacol. Sci. 22, 27.
  5. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
  6. Isom, L.L. (2001) Neuroscientist 7, 42.
  7. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 575.
  8. Catterall, W.A. et al. (2005) Pharmacol. Rev. 57, 397.
  9. Yang, Y. et al. (2004) J. Med. Genet. 41, 171.
  10. Cummins, T.R. et al. (2004) J. Neurosci. 24, 8232.
  11. Dray, A. (2008) Br. J. Anaesth. 101, 48.
Scientific background

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.94-5. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9)2-3.

Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,8.

NaV1.7 is predominantly expressed in dorsal root ganglions (DRG) of the peripheral nervous system. Dominant gain of function mutations in the NaV1.7 gene are associated with erythermalgia (a rare autosomal disease characterized by sporadic burning pain accompanied by redness and heat in the extremities).9-11 Loss of function mutations in NaV1.7 channels leads to complete ablation of pain perception in humans.11 These recent findings highlight the role of this NaV isoform and the subset of DRG neurons that express this channel in physiological pain sensation.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat NaV1.7 channel. Guinea pig Anti-NaV1.7 (SCN9A) Antibody (#AGP-057) raised in guinea pigs can be used in western blot analysis. It has been designed to recognize NaV1.7 from rat, human and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-NaV1.7 (SCN9A) Antibody (#ASC-008) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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