Anti-SCN11A (NaV1.9) Antibody

PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha
    Cat #: ASC-017
    Alternative Name PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, r
    May also work in: m*
      • Peptide CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat NaV1.9 (Accession O88457). Intracellular, C-terminus.
        Anti-SCN11A (NaV1.9) Antibody
    Accession (Uniprot) Number O88457
    Gene ID 29701
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 15/18 amino acid residues identical; human - 14/18 amino acid residues identical.
    RRID AB_2040200.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
      • Anti-SCN11A (NaV1.9) Antibody
        Western blot analysis of rat DRG lysates:
        1. Anti-SCN11A (NaV1.9) Antibody (#ASC-017), (1:200).
        2. Anti-SCN11A (NaV1.9) Antibody, preincubated with the negative control antigen.
      • Anti-SCN11A (NaV1.9) Antibody
        Expression of NaV1.9 in rat embryo DRG
        Immunohistochemical staining of formalin frozen rat embryo dorsal root ganglion (DRG) sections with Anti-SCN11A (NaV1.9) Antibody (#ASC-017). A. Cells within the DRG were stained (see solid line frame enlarged in B) as well as fibers and the area of entry of dorsal root into the spinal cord (see dashed line frame enlarged in C). DAPI is used as the counterstain.
        Human trigeminal ganglia and dental pulp (1:100) (Wells, J.E. et al. (2007) J. Endodontics 33, 1172.).
      • Rat dorsal root ganglion (DRG) primary culture (1:500).
    References
    • 1. Wu, L. et al. (2002) NeuroReport 13, 2547.
    • 2. Baker, M.D. et al. (2001) Trends. Pharmacol. Sci. 22, 27.
    • 3. Lai, J. et al (2003) Curr. Opin. Neurobiol. 13, 291.
    • 4. Fang, X. et al (2002) J. Neurosci. 22, 7425.
    • 5. Fjell, J. et al (2000) NeuroReport 11, 199.
    • 6. Blum, R. et al (2002) Nature 419, 687.
      • Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.9.2-3 Expression of the α subunit is developmentally and tissue specific.

        The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.4-5 TTX-resistant channels have been suggested to play an important role in nociceptive transmission. Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.9 channel (also called SCN11A, NAN or SNS2) is preferentially expressed in small-diameter DRG neurons.4-5

        Recently, it was shown that BDNF activates NaV1.9 channels in the hippocampus suggesting that its expression is not restricted to DRG alone.6

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/11/2019

    Anti-SCN11A (NaV1.9) Antibody (#ASC-017) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize NaV1.9 channel from rat, human, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Rat brain neurolemma lysate.
        Murenzi, E. et al. (2016) Neurotoxicology In press.
      • Rat sciatic nerve and rat DRG (1:500).
        Su, Y.Y. et al. (2013) J. Neurosci. 33, 17884.
      • Rat L4-6 DRG lysates (1:200).
        Yu, Y.Q. et al. (2011) PLoS ONE 6, e19865.
      • Rat DRG sections (1:300).
        Qiu, F. et al. (2016) Neurochem. Res. 41, 1587.
      • Rat L4-6 DRG sections (1:200).
        Yu, Y.Q. et al. (2011) PLoS ONE 6, e19865.
      • Human trigeminal ganglia and dental pulp (1:100).
        Wells, J.E. et al. (2007) J. Endodontics 33, 1172.
    Related Products
        • Guinea pig Anti-SCN11A (NaV1.9) Antibody (#AGP-030) is raised in guinea pig and can be used in colocalization studies in conjunction with any of our antibodies raised in rabbit. This antibody has been raised against the same epitope as #ASC-017.