Anti-NaVβ4 (extracellular) Antibody

Sodium channel subunit beta-4
    Cat #: ASC-044
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)KNDKSDPKVRVKDD, corresponding to amino acid residues 85-98 of rat NaVβ4 (Accession Q7M730). Extracellular, N-terminus.
    Accession (Uniprot) Number Q7M730
    Gene ID 315611
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 11/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc
    Western blot
    Western blot analysis of  rat brain (lanes 1 and 5), rat cortex (lane 2 and 6), mouse brain (lanes 3 and 7) and SH-SY5Y (lanes 4 and 8) lysates:
    1-4. Anti-Navβ4 (extracellular) Antibody (#ASC-044), (1:800).
    5-8. Anti-Navβ4 (extracellular) Antibody preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of NaVβ4 in rat DRG
    Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-NaVβ4 (extracellular) Antibody (#ASC-044) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. NaVβ4 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.
    Live cell imaging / Immunocytochemistry
    Expression of NaVβ4 in rat PC12 cells
    Immunocytochemical staining of intact living rat Pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-NaVβ4 (extracellular) Antibody (#ASC-044), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.
    References
    1. Wu, L. et al. (2002) NeuroReport 13, 2547.
    2. Fang, Xet al. (2002) J. Neurosci22, 7425.
    3. Fjell, Jet al. (2000) NeuroReport 11, 199.
    4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
    5. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
    6. Isom, L.L. (2001) Neuroscientist 7, 42.
    7. Catterall, W.Aet al. (2003) Pharmacol. Rev. 55, 575.
    8. Medeiros-Domingo, A. et al. (2007) Circulation 116, 134.
    9. Oyama, F. et al. (2006) J. Neurochem. 98, 518.
    Scientific background

    Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named Nav1.1-Nav1.94,5.  The Nav channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9) 2,3. Mammalian Na+ channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,7.

    Navβ4 has been associated with Long QT syndrome8 and with Huntington's disease, showing a distinct down regulation of NaVβ4 in the presymptomatic stage of HD mice, whereas other voltage-gated ion channel subunits were later decreased9.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/10/2018

    Anti-NaVβ4 (extracellular) Antibody (#ASC-044) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting NaVβ4 in living cells. It has been designed to recognize NaVβ4 from rat, human, and mouse samples.

    For research purposes only, not for human use