- Peptide (C)KSDYLKHSTFDQDS, corresponding to amino acid residues 207-220 of rat P2X2 with replacement of cysteine 214 (C214) with serine (*S) (Accession P49653). Extracellular.
Will not recognize human P2X2.
- Rat and mouse brain membranes. Rat PC12 cell line (1:200). Addition of 0.1-0.5% Triton x-100 to the antibody solution is recommended.
- Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and PC12 cells (lanes 3 and 6):1-3. Anti-P2X2 Receptor (extracellular) Antibody (#APR-025), (1:200).
4-6. Anti-P2X2 Receptor (extracellular) Antibody, preincubated with P2X2 Receptor (extracellular) Blocking Peptide (#BLP-PR025).
- Live rat pheochromocytoma cell line (PC12), (1:50).
The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.
The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.
Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop. All P2X subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9
The various mammalian P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X2 receptor subtypes has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.