Anti-P2Y2 Receptor Antibody

P2Y purinoceptor 2, P2RY2, P2U1
    Cat #: APR-010
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)KPAY GTTGL PRAKR KSVR, corresponding to residues 227-244 of rat P2RY2 (Accession P41232). 3rd intracellular loop (i3) between TM5 and TM6 domains.
    Accession (Uniprot) Number P41232
    Gene ID 29597
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 18/19 amino acid residues identical; human - 17/19 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ifc, ih, ip, wb
    Western blot
    Western blot analysis of rat brain (lanes 1, 3) and lung (lanes 2, 4) membranes:
    1,2. Anti-P2Y2 Receptor Antibody (#APR-010), (1:200).
    3,4. Anti-P2Y2 Receptor Antibody, preincubated with the control peptide antigen.
    Mouse mIMCD-K2 cell lysate (4 μg/ml) (Rajagopal, M. et al. (2011) Am. J. Physiol. 301, F544.).
    Immunoprecipitation
    Rat DRGs (Arthur, D.B. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 19138.).
    Immunohistochemistry
    Rat dorsal root ganglia (1:300) (Mo, G. et al. (2013) Mol. Pharmacol. 83, 640.).
    Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
    Immunocytochemistry
    Rat PC12 cells (1:100) (Arthur, D.B. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 19138.).
    Indirect flow cytometry
    Rat osteoclasts (8 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
    The control antigen is not suitable for this application.
    References
    1. Wang, L. et al. (2002) J. Cardiovas. Pharmacol40, 841.
    2. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther307, 809.
    3. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev50, 413.
    4. Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci24, 52.
    5. Di Virgilio, F. et al. (2001) Blood 97, 587.
    6. Yerxa, B.R. et al. (2002) J. Pharmacol. Exp. Ther302, 871.
    Scientific background

    The P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation.1

    The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4P2Y6P2Y11P2Y12P2Y13, and the UDP-glucose receptor, now renamed P2Y14 .2-4

    The P2Y2 receptor is abundantly expressed in the apical membranes of several epithelial tissues including airway.5 It has become a leading candidate for the treatment of cystic fibrosis. Activation of P2Y2 receptor increases Cl- efflux and mucociliary clearance, effects that might relieve symptoms of cystic fibrosis. P2Y2 agonist therapy is tested in an attempt to bypass the dysfunctioning CFTR channel. Several P2Y2 receptor agonists, especially INS37217, being evaluated as possible targets for the treatment of cystic fibrosis.6

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-P2Y2 Receptor Antibody
    Expression of P2Y2 receptor in rat cortical neurons.Immunocytochemical staining of rat cortical neurons using Anti-P2Y2 Receptor Antibody (#APR-010). P2Y2 receptor immuno-colocalizes with PSD-95, the synaptic marker.Adapted from Choi, R.C. et al. (2013) Mol. Pharmacol. 84, 50. with permission of the American Society for Pharmacology & Experimental Therapeutics.

    Last update: 11/11/2018

    Anti-P2Y2 Receptor Antibody (#APR-010) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2RY2 in rat, mouse, and human samples.

    For research purposes only, not for human use
    Citations
    Published figures using this product
    Immuno-colocalization of KCa3.1 and P2Y2 in human ovarian carcinoma
    Immunohistochemical staining of human ovarian carcinoma biopsies using Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody (#ALM-051) and rabbit Anti-P2Y2 Receptor Antibody (#APR-010). Positive co-expression is detected in ovarian neoplastic cells (merged image). Expression of KCa3.1 is also observed in stromal cells (*); however, P2RY2 does not co-localize with KCa3.1 in these cells.
    Adapted from Robles-Martinez, L. et al. (2017) Sci. Rep. 7, 4340. with permission of Nature Publishing Group.
    Western blot citations
    1. Mouse brain lysate.
      Cui, J.D. et al. (2016) Chem. Biol. Interact. 259, 291.
    2. Mouse bone marrow lysate.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    3. Mouse bone lysate and osteoblast MOB-C cell lysate.
      Seref-Ferlengez, Z. et al. (2016) PLoS ONE 11, e0155107.
    4. Rat cardiac fibloblast lysate (1:200).
      Certal, M. et al. (2015) Cell Calcium 58, 518.
    5. HEK 293 transfected cell lysates.
      Yashima, S. et al. (2015) J. Biochem. 158, 155.
    6. Human MCF-7 cell lysate (1:200).
      Chadet, S. et al. (2014) Carcinogenesis 35, 1238.
    7. Rat cortical neuron lysate (1:1000).
      Choi, R.C. et al. (2013) Mol. Pharmacol. 84, 50.
    8. Mouse cerebral synaptosome.
      Naviaux, R.K. et al. (2013) PLoS ONE 8, e57380.
    9. Mouse mIMCD-K2 cell lysate (4 μg/ml).
      Rajagopal, M. et al. (2011) Am. J. Physiol. 301, F544.
    Immunoprecipitation citations
    1. Mouse bone marrow lysate.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. Rat DRGs.
      Arthur, D.B. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 19138.
    Immunohistochemistry citations
    1. Human ovarian tumor sections.
      Robles-Martinez, L. et al. (2017) Sci. Rep. 7, 4340.

    2. Mouse hippocampal slices (1:50).
      Takano, T. et al. (2014) Glia 62, 78.
    3. Rat dorsal root ganglia (1:300).
      Mo, G. et al. (2013) Mol. Pharmacol. 83, 640.
    4. Human eye tissue.
      Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.
    Immunocytochemistry citations
    1. Rat cardiac fibroblasts (1:100).
      Certal, M. et al. (2015) Cell Calcium 58, 518.
    2. Rat cortical neurons (1:200).
      Choi, R.C. et al. (2013) Mol. Pharmacol. 84, 50.
    3. HEK-293T cells transfected with rat P2Y2R (1:200).
      Choi, R.C. et al. (2013) Mol. Pharmacol. 84, 50.
    4. Rat PC12 cells (1:100).
      Arthur, D.B. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 19138.
    Indirect flow cytometry citations
    1. Mouse bone marrow cells.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. Rat osteoclasts (8 μg/ml).
      Alvarenga, E.C. et al. (2010) Bone 46, 355.
    More product citations
    1. Traves, P.G. et al. (2013) J. Immunol. 190, 4226.
    2. Zerpa, H. et al. (2013) Eur. J. Pharmacol. 702, 242.
    3. Mortensen, S.P. et al. (2012) J. Physiol. 590, 6227.
    Related Products
      • Antibodies for live cell imaging experiments
        1. Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102). This antibody detects P2Y2 in an extracellular domain and can be used to detect the receptor in live cell imaging.