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Anti-P2Y2 Receptor Antibody

P2Y purinoceptor 2, P2RY2, P2U1
Cat #: APR-010
Alternative Name P2Y purinoceptor 2, P2RY2, P2U1
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)KPAY GTTGL PRAKR KSVR, corresponding to residues 227-244 of rat P2RY2 (Accession P41232). 3rd intracellular loop (i3) between TM5 and TM6 domains.
    Accession (Uniprot) Number P41232
    Gene ID 29597
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 18/19 amino acid residues identical; human - 17/19 amino acid residues identical.
    RRID AB_2040078.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, ip, wb
    Western blot
    • Western blot analysis of rat brain (lanes 1, 3) and lung (lanes 2, 4) membranes:
      Western blot analysis of rat brain (lanes 1, 3) and lung (lanes 2, 4) membranes:
      1,2. Anti-P2Y2 Receptor Antibody (#APR-010), (1:200).
      3,4. Anti-P2Y2 Receptor Antibody, preincubated with P2Y2 Receptor Blocking Peptide (#BLP-PR010).
    • Mouse mIMCD-K2 cell lysate (4 μg/ml) (Rajagopal, M. et al. (2011) Am. J. Physiol. 301, F544.).
    Immunoprecipitation
    • Rat DRGs (Arthur, D.B. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 19138.).
    Immunohistochemistry
    • Rat dorsal root ganglia (1:300) (Mo, G. et al. (2013) Mol. Pharmacol. 83, 640.).
    • Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
    Immunocytochemistry
    • Rat PC12 cells (1:100) (Arthur, D.B. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 19138.).
    Indirect flow cytometry
    • Rat osteoclasts (8 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
    • The control antigen is not suitable for this application.
    References
    1. Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841.
    2. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
    3. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
    4. Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
    5. Di Virgilio, F. et al. (2001) Blood 97, 587.
    6. Yerxa, B.R. et al. (2002) J. Pharmacol. Exp. Ther. 302, 871.
    Scientific background

    The P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation.1

    The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4P2Y6P2Y11P2Y12P2Y13, and the UDP-glucose receptor, now renamed P2Y14 .2-4

    The P2Y2 receptor is abundantly expressed in the apical membranes of several epithelial tissues including airway.5 It has become a leading candidate for the treatment of cystic fibrosis. Activation of P2Y2 receptor increases Cl- efflux and mucociliary clearance, effects that might relieve symptoms of cystic fibrosis. P2Y2 agonist therapy is tested in an attempt to bypass the dysfunctioning CFTR channel. Several P2Y2 receptor agonists, especially INS37217, being evaluated as possible targets for the treatment of cystic fibrosis.6

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-P2Y2 Receptor Antibody
    Expression of P2Y2 receptor in rat cortical neurons.Immunocytochemical staining of rat cortical neurons using Anti-P2Y2 Receptor Antibody (#APR-010). P2Y2 receptor immuno-colocalizes with PSD-95, the synaptic marker.Adapted from Choi, R.C. et al. (2013) Mol. Pharmacol. 84, 50. with permission of the American Society for Pharmacology & Experimental Therapeutics.

    Last update: 16/08/2020

    Anti-P2Y2 Receptor Antibody (#APR-010) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2RY2 in rat, mouse, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Published figures using this product
    • Multiplex staining of KCa3.1 and P2Y2 in human ovarian carcinoma
      Multiplex staining of KCa3.1 and P2Y2 in human ovarian carcinoma
      Immunohistochemical staining of human ovarian carcinoma biopsies using Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody (#ALM-051) and rabbit Anti-P2Y2 Receptor Antibody (#APR-010). Positive co-expression is detected in ovarian neoplastic cells (merged image). Expression of KCa3.1 is also observed in stromal cells (*); however, P2RY2 does not co-localize with KCa3.1 in these cells.
      Adapted from Robles-Martinez, L. et al. (2017) Sci. Rep. 7, 4340. with permission of Nature Publishing Group.
    KO validation citations
    1. Western blot analysis of human umbilical vein endothelial cells (HUVECs). Tested in siRNA-treated cells.
      Sathanoori, R. et al. (2017) Cell. Mol. Life Sci. 74, 731.
    Western blot citations
    1. Human umbilical vein endothelial cells (HUVECs). Tested in siRNA-treated cells.
      Sathanoori, R. et al. (2017) Cell. Mol. Life Sci. 74, 731.
    2. Rat osteoclast lysate.
      Ellegaard, M. et al. (2017) Purinergic Signal. 13, 545.
    3. Rat primary astrocyte culture lysate.
      Paniagua-Herranz, L. et al. (2017) Front. Pharmacol. 8, 937.
    4. Human esophageal epithelial cell lysate.
      Wu, L. et al. (2017) J. Gastroenrol. Hepatol. 32, 1341.
    5. Mouse brain lysate.
      Cui, J.D. et al. (2016) Chem. Biol. Interact. 259, 291.
    6. Mouse bone marrow lysate.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    7. Mouse bone lysate and osteoblast MOB-C cell lysate.
      Seref-Ferlengez, Z. et al. (2016) PLoS ONE 11, e0155107.
    8. Rat cardiac fibloblast lysate (1:200).
      Certal, M. et al. (2015) Cell Calcium 58, 518.
    9. HEK 293 transfected cell lysates.
      Yashima, S. et al. (2015) J. Biochem. 158, 155.
    10. Human MCF-7 cell lysate (1:200).
      Chadet, S. et al. (2014) Carcinogenesis 35, 1238.
    11. Rat cortical neuron lysate (1:1000).
      Choi, R.C. et al. (2013) Mol. Pharmacol. 84, 50.
    12. Mouse cerebral synaptosome.
      Naviaux, R.K. et al. (2013) PLoS ONE 8, e57380.
    13. Mouse mIMCD-K2 cell lysate (4 μg/ml).
      Rajagopal, M. et al. (2011) Am. J. Physiol. 301, F544.
    Immunoprecipitation citations
    1. Mouse bone marrow lysate.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. Rat DRGs.
      Arthur, D.B. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 19138.
    Immunohistochemistry citations
    1. Rat kidney sections.
      Palygin, O. et al. (2018) Punergic Signal. 14, 485.
    2. Mouse anterior pole eye sections.
      Fonseca, B. et al. (2017) Front. Pharmacol. 8, 719.
    3. Human ovarian tumor sections.
      Robles-Martinez, L. et al. (2017) Sci. Rep. 7, 4340.
    4. Rat spinal cord sections (1:150).
      Shi, Y. et al. (2017) Gene 627, 263.
    5. Human esophagus sections.
      Wu, L. et al. (2017) J. Gastroenrol. Hepatol. 32, 1341.
    6. Mouse hippocampal slices (1:50).
      Takano, T. et al. (2014) Glia 62, 78.
    7. Rat dorsal root ganglia (1:300).
      Mo, G. et al. (2013) Mol. Pharmacol. 83, 640.
    8. Human eye tissue.
      Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.
    Immunocytochemistry citations
    1. Rat cardiac fibroblasts (1:100).
      Certal, M. et al. (2015) Cell Calcium 58, 518.
    2. Rat cortical neurons (1:200).
      Choi, R.C. et al. (2013) Mol. Pharmacol. 84, 50.
    3. HEK-293T cells transfected with rat P2Y2R (1:200).
      Choi, R.C. et al. (2013) Mol. Pharmacol. 84, 50.
    4. Rat PC12 cells (1:100).
      Arthur, D.B. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 19138.
    Indirect flow cytometry citations
    1. Mouse bone marrow cells.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. Rat osteoclasts (8 μg/ml).
      Alvarenga, E.C. et al. (2010) Bone 46, 355.
    More product citations
    1. Traves, P.G. et al. (2013) J. Immunol. 190, 4226.
    2. Zerpa, H. et al. (2013) Eur. J. Pharmacol. 702, 242.
    3. Mortensen, S.P. et al. (2012) J. Physiol. 590, 6227.
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