Free shipping starts now, no minimum, no coupons required!

Anti-RAGE (extracellular) Antibody

Advanced Glycosylation End Product-Specific Receptor, AGER, Receptor For Advanced Glycosylation End Products, SCARJ1
Cat #: ANR-176
Alternative Name Advanced Glycosylation End Product-Specific Receptor, AGER, Receptor For Advanced Glycosylation End Products, SCARJ1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GKETKSNYRVRVYQIPGK, corresponding to amino acid residues 106 - 123 of human RAGE (Accession Q15109). Extracellular, N-terminus.
Accession (Uniprot) Number Q15109
Gene ID 177
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – 17/18 amino acid residues identical.
RRID AB_2876845
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity This antibody will recognize both the membrane-bound and the soluble form of the RAGE receptor.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat lung membranes (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):
    Western blot analysis of rat lung membranes (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):
    1, 2. Anti-RAGE (extracellular) Antibody (#ANR-176), (1:600).
    3, 4. Anti-RAGE (extracellular) Antibody, preincubated with RAGE (extracellular) Blocking Peptide (#BLP-NR176).
  • Western blot analysis of human PANC-1 pancreatic carcinoma cell line lysate (lanes 1 and 5), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 6), human THP-1 monocytic leukemia cell line lysate (lanes 3 and 7) and mouse BV-2 microglia cell line lysate (lanes 4 and 8):
    Western blot analysis of human PANC-1 pancreatic carcinoma cell line lysate (lanes 1 and 5), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 6), human THP-1 monocytic leukemia cell line lysate (lanes 3 and 7) and mouse BV-2 microglia cell line lysate (lanes 4 and 8):
    1-4. Anti-RAGE (extracellular) Antibody (#ANR-176), (1:600).
    5-8. Anti-RAGE (extracellular) Antibody, preincubated with RAGE (extracellular) Blocking Peptide (#BLP-NR176).
Indirect flow cytometry
  • Cell surface detection of RAGE in live intact mouse BV-2 microglia cell line:
    Cell surface detection of RAGE in live intact mouse BV-2 microglia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-RAGE (extracellular) Antibody (#ANR-176), (2.5 µg) + goat-anti-rabbit-FITC.
References
  1. Serveaux-Dancer, M. et al. (2019) Dis. Markers 2019, 2067353.
  2. Barbezier, N. et al. (2014) Ann. Biol. Clin. 72, 669.
  3. Yonekura, H. et al. (2003) Biochem. J. 370, 1097.
  4. Oczypok, E.A. et al. (2017) Paediatr. Respir. Rev. 23, 40.
Scientific background

Receptor for advanced glycation end-products (RAGE) is a cell surface transmembrane multi-ligand receptor belonging to the immunoglobulin superfamily, encoded by the AGER gene.

The link between the RAGE and its multiple ligands, including Glycation End Product (AGEs) triggers a cascade of intracellular events and transcription of genes, as well as other reactions such as the formation of oxidative stress. These reactions are involved in neurological and vascular complications of diabetes, Alzheimer's Disease, arthritis, and some cancers. In addition, chronic stimulation of RAGE triggers different signaling pathways and reactions1,2.

RAGE is a 35 kDa protein mainly expressed in the lungs and exists under two main forms: membrane-bound RAGE (mRAGE) and soluble RAGE (sRAGE). Membrane-bound RAGE has three domains: an extracellular domain that recognizes and binds RAGE ligands, a hydrophobic transmembrane domain, and a charged cytoplasmic domain that functions in intracellular signaling. Soluble RAGE contains only the extracellular domain and is a product of either alternative splicing events or the proteolytic cleavage of mRAGE. Because it can bind ligands but cannot transduce signals intracellularly, sRAGE is a decoy receptor that sequesters RAGE ligands and prevents inflammatory responses3.

Use of sRAGE, RAGE blocking antibody, or a small molecule inhibitors to interfere with RAGE signaling may become novel therapeutic approaches to inhibit RAGE-mediated disease4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Widget not in any sidebars
Last update: 20/10/2020

Anti-RAGE (extracellular) Antibody (#ANR-176) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize RAGE from human, rat, and mouse samples. This antibody will recognize both the membrane-bound and the soluble form of the RAGE receptor.

For research purposes only, not for human use
Shipping and Ordering information