Anti-Rat TRPV1 (VR1) (extracellular) Antibody

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1
    Cat #: ACC-029
    Alternative Name Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: r
      • Peptide (C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C616) with serine (*S) (Accession O35433). 3rd extracellular loop.
        Anti-Rat TRPV1 (VR1) (extracellular) Antibody
    Accession (Uniprot) Number O35433
    Gene ID 83810
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Specific to rat TRPV1.
    RRID AB_2040258.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Recognizes TRPV1 from rat samples only.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
      • Anti-Rat TRPV1 (VR1) (extracellular) Antibody
        Western blot analysis of TRPV1 in rat DRG:
        1. Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029), (1:200).
        2. Anti-Rat TRPV1 (VR1) (extracellular) Antibody, preincubated with the control peptide antigen.
      • Anti-Rat TRPV1 (VR1) (extracellular) Antibody
        Expression of TRPV1 in rat DRG
        Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029), (1:100) followed by goat anti-rabbit-ATTO-594 secondary antibody (red). TRPV1 is expressed in medium and small DRG neurons. Hoechst 33342 is used as the counterstain (blue).
      • F11, rat DRG cell line (1:1000) (Goswami, C. et al. (2010) J. Cell Sci. 123, 2045.).
    References
      • TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1

        According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5

        TRPV1 channel has many activators among them heat, protons, vanilloids like capsaicin, reiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.6,7

        TRPV1 is expressed predominantly in nociceptors and in sensory neurons.

        Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-Rat TRPV1 (VR1) (extracellular) Antibody
    Expression of TRPV1 in rat TGNs.Immunocytochemical staining of living rat trigeminal ganglia neurons (TGNs). Extracellular staining of cells using Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029). Adapted from Meng, J. et al. (2016) Sci. Rep. 6, 21226. with permission of Nature Publishing Group.

    Last update: 10/11/2019

    Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029) is a highly specific antibody directed against an extracellular epitope of the rat TRPV1 channel. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize TRPV1 from rat samples only.

    For research purposes only, not for human use
    Citations
      • Rat trigeminal ganglion neurons.
        Meng, J. et al. (2016) Sci. Rep. 6, 21226.
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