Anti-Rat TRPV1 (VR1) (extracellular)-ATTO-488 Antibody

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1
    Cat #: ACC-029-AG
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: r
    Immunogen
    Peptide (C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C616) with serine (*S) (Accession O35433). 3rd extracellular loop.
    Accession (Uniprot) Number O35433
    Gene ID 83810
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Specific to rat TRPV1.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity Recognizes TRPV1 from rat only.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-029), and immunohistochemistry (labeled antibody).
    Applications: ic, ih, lci
    Immunohistochemistry
    Immuno-colocalization of TRPV1 and NaV1.8 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-Rat TRPV1 (VR1) (extracellular)-ATTO-488 Antibody (#ACC-029-AG), (green), (1:60) and Anti-NaV1.8 (SCN10A)-ATTO-594 Antibody (#ASC-016-AR), (red), (1:60). A. TRPV1 staining. B. NaV1.8 staining. C. Merge of A and B demonstrates partial co-localization of TRPV1 and NaV1.8 channels. Nuclei stained using DAPI as the counterstain (blue).
    Expression of TRPV1 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Rat TRPV1 (VR1) (extracellular )-ATTO-488 Antibody (#ACC-029-AG), (1:50), (green). TRPV1 is expressed in medium and small DRG neurons. Hoechst 33342 is used as the counterstain (blue).
    Live cell imaging / Immunocytochemistry
    Expression of TRPV1 in rat PC12 cells
    Immunocytochemical staining of intact living Pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-Rat TRPV1 (VR1) (extracellular)-ATTO-488 Antibody (#ACC-029-AG), (1:25), (green). B. Live view of the cell. C. Merge images of A and B.
    References
    1. Montell, C. et al. (2002) Mol. Cell9, 229.
    2. Clapham, D.E. (2003) Nature 426, 517.
    3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol14, 362.
    4. Clapham, D.E. et al. (2003) Pharmacol. Rev55, 591.
    5. Gunthorpe, M.J et al. (2002) Trends. Pharmacol. Sci. 23, 183.
    6. Ross, R.Aet al. (2003) Br. J. Pharmacol. 140, 790.
    7. Tominaga, Met al. (1998) Neuron 21, 531.
    8. Agopyan, Net al. (2004) Am. J. Physiol. 286, L563.
    9. Reilly, C.Aet al. (2003) Toxicol. Sci. 73, 170.
    Scientific background

    TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1

    According to IUPHAR the TRP family comprises three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5

    TRPV1 channel has many activators; among them heat, protons, vanilloids like capsaicin, resiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.6,7 TRPV1 is expressed predominantly in nociceptors and in sensory neurons.

    Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-Rat TRPV1 (VR1) (extracellular)-ATTO-488 AntibodyImmuno-colocalization of TRPV1 and mGluR5 in rat DRGImmunohistochemical staining of rat dorsal root ganglion using Anti-Rat TRPV1 (VR1) (extracellular)-ATTO-488 Antibody (#ACC-029-AG), (1:60) and Anti-mGluR5 (extracellular)-ATTO-594 Antibody (#AGC-007-AR), (1:60).  A. TRPV1 staining (green). B. mGluR5 staining of the same section (red). C. Merge of A and B demonstrates co-localization of TRPV1 and mGluR5 in DRG cells. Nuclei were stained using DAPI as the counterstain (blue).

    Last update: 23/10/2018

    Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029) is a highly specific antibody directed against an extracellular epitope of the rat TRPV1 channel. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize TRPV1 from rat samples only.

    Anti-Rat-TRPV1 (VR1) (extracellular)-ATTO-488 Antibody (#ACC-029-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Rat-TRPV1 (VR1) (extracellular)-ATTO-488 Antibody has been tested in immunohistochemical and live cell imaging applications and is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use
    Citations
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