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Anti-sloβ3 (KCNMB3) (extracellular) Antibody

Ca2+-activated K+ channel subunit β3, BKCa β3, BK beta 3, Maxi K channel β3, Charybdotoxin receptor subunit beta-3

Cat #: APC-068
Alternative Name Ca2+-activated K+ channel subunit β3, BKCa β3, BK beta 3, Maxi K channel β3, Charybdotoxin receptor subunit beta-3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)HYDEEAIRTNPK, corresponding to amino acid residues 134-145 of rat KCNMB3 (Accession A7VL23). Extracellular loop.
Accession (Uniprot) Number A7VL23
Gene ID 310303
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 11/12 amino acid residues identical.
RRID AB_2341029.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat pancreas membranes (lanes 2 and 4):
    Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat pancreas membranes (lanes 2 and 4):
    1,2. Anti-sloβ3 (KCNMB3) (extracellular) Antibody (#APC-068), (1:1000).
    3,4. Anti-sloβ3 (KCNMB3) (extracellular) Antibody, preincubated with sloβ3/KCNMB3 (extracellular) Blocking Peptide (#BLP-PC068).
Immunohistochemistry
  • Expression of KCNMB3 in rat hippocampus.
    Expression of KCNMB3 in rat hippocampus.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with  Anti-sloβ3 (KCNMB3) (extracellular) Antibody (#APC-068), (1:300), followed by goat anti-rabbit-Alexa-488. A. Staining in the hippocampal CA3 region, showed immunoreactivity (green) in cortical neurons (arrows). B. Pre-incubation of the antibody with sloβ3 (KCNMB3) (extracellular) Blocking Peptide (BLP-PC068), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Xia, X.M. et al. (1999) J. Neurosci. 19, 5255.
  2. Shen K.Z. et al. (1994) Pflugers Arch 408, 98.
  3. Ramanathan, K. et al. (1999) Science 283, 215.
  4. Orio, P. et al. (2002) News Physiol. Sci. 17, 156.
  5. Lee, U.S. et al. (2009) J. Physiol. 587, 1481.
  6. Zeng, X. et al. (2008) J. Gen. Physiol. 132, 115.
  7. Lorenz, S. et al. (2007) Am. J. Med. Genet. B. Neuropsychiatr. Genet. 144B, 10.
Scientific background

Large-conductance, voltage- and Ca2+-activated K+ channels, also known as the BK channels, are widely expressed channels that couple changes in submembrane Ca2+ concentration to the regulation of electrical excitability1. BK channels are formed from four α-subunits arising from the Slowpoke (Slo) gene product2. In addition, in smooth muscle and cochlea, an accessory β-subunit can regulate BK channel gating profoundly. At present, four β subunits have been cloned in mammals. β subunits alter the Ca2+ sensitivity and gating kinetics of BK channels, greatly contributing to BK channel diversity. On the other hand, they modify the BK channel pharmacological properties, changing toxin binding and acting as receptors for drugs3.

Regulatory β subunits share a putative membrane topology, with two transmembrane segments connected by a 120- residue extracellular loop and with NH2 and COOH terminals oriented toward the cytoplasm4. Each β subunit has a tissue-specific expression and modulates channel function uniquely which provides a major mechanism for diverse BK channel phenotypes in various tissues.

β3 is highly expressed in kidney, heart, and brain5. The β3 gene (KCNMB3) associates with Slo1 α subunits and regulate BK channel function. In humans, the β3 gene contains four N-terminal alternative exons that produce four functionally distinct β3 subunits, β3a-d. Three variants, β3a-c, exhibit kinetically distinct inactivation behaviors6. A mutation in the β3 gene is linked to idiopathic epilepsy7.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 22/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat sloβ3. Anti-sloβ3 (KCNMB3) (extracellular) Antibody (#APC-068) can be used in western blot analysis. It has been designed to recognize sloβ3 from rat and mouse samples.

For research purposes only, not for human use
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