Anti-sloβ2 (KCNMB2) Antibody

Ca2+-activated K+ channel subunit β2, BKCa β2, BK beta 2, Maxi K channel β2, Charybdotoxin receptor subunit beta-2
Cat #: APC-034
Alternative Name Ca2+-activated K+ channel subunit β2, BKCa β2, BK beta 2, Maxi K channel β2, Charybdotoxin receptor subunit beta-2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, r
    May also work in: m
      • Peptide (C)RHDEKRNIYQKIRDHDLLD, corresponding to amino acid residues 14-32 of human KCNMB2 (Accession Q9Y691). Intracellular, N-terminal part.
    Accession (Uniprot) Number Q9Y691
    Gene ID 10242
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, dog - identical; mouse - 18/19 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, wb
    May also work in: ifc, ip
      • Western blot analysis of rat kidney (lanes 1 and 3) and heart (lanes 2 and 4) membranes:
        1,2. Anti-sloβ2 (KCNMB2) Antibody (#APC-034), (1:200).
        3,4. Anti-sloβ2 (KCNMB2) Antibody, preincubated with the control peptide antigen.
      • Expression of KCNMB2 in rat hippocampus
        Immunohistochemical staining of rat hippocampus using Anti-sloβ2 (KCNMB2) Antibody (#APC-034). A. KCNMB2 appears in the pyramidal layer (green). B. Staining of interneurons with mouse anti-parvalbumin (PV, red). C. Confocal merge of KCNMB2 and PV demonstrates presence of KCNMB2 also in PV (GABA containing) cells (orange).
      • Rat pinealocytes (Mizutani, H. et al. (2016) Am. J. Physiol. 310, C740.)
      • Wallner, M. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 4137.
      • Xia, X.M. et al. (1999) J. Neurosci. 19, 5255.
      • Orio, P. et al. (2002) News Physiol. Sci. 17, 156.
      • sloβ2 is a member the regulatory β subunit family that controls the activity of the large conductance Ca2+-activated K+ channel KCa1.1. This family includes four members with a shared topology: two trans-membrane domains, short intracellular N- and C-termini and a large extracellular region and a distinct tissue distribution.

        sloβ2 expression is relatively broad and includes expression in the brain, heart, kidney, adrenal chromaffin cells and ovary.

        The KCa1.1 K+ channel can be activated by either an increase in intracellular Ca2+ concentration or by membrane depolarization. The regulatory β subunits increase the sensitivity of the pore-forming KCa1.1 subunit to Ca2+ and membrane voltage and they may also change the channel pharmacology.

        The sloβ2 subunit is unique in that it is able to induce a rapid and complete inactivation of the KCa1.1 channel in a manner that closely resembles the ball-and-chain inactivation of the voltage-dependent K+ (KV) channels. In other words, the inactivation is dependent on a sequence in the N-terminal part of the sloβ2 subunit that appears to block the mouth of the ion permeation pathway.

        The physiological significance of the sloβ2 subunit is not clear, but it appears to participate in the inactivation of the KCa1.1 channel in hippocampal CA1 neurons and adrenal chromaffin cells.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/06/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human sloβ2. Anti-sloβ2 (KCNMB2) Antibody (#APC-034) can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize sloβ2 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Human dermal fibroblasts (1:200).
        Kicinska, A. et al. (2016) Biochem. J. 473, 4457.
      • Mitochondria and mitoplast from human endothelial EA.hy926 cells (1:200).
        Bednarczyk, P. et al. (2013) Am. J. Physiol. 304, H1415.
      • Rat pinealocytes.
        Mizutani, H. et al. (2016) Am. J. Physiol. 310, C740.
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