Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody

Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1
    Cat #: APC-107
    Alternative Name Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)STANRPNRPKSRESRDK, corresponding to amino acid residues 1184-1200 of mouse KCNMA1 (Accession number Q08460). Intracellular, C-terminal part.
    • Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody
    Accession (Uniprot) Number Q08460
    Gene ID 16531
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - identical; human, bovine, chicken, dog - 16/17 amino acid residues identical.
    RRID AB_2040091.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.6 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
    Western blot
    • Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody
      Western blot analysis of rat brain membranes:
      1. Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107), (1:500). 
      2. Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody, preincubated with the negative control antigen.
    Immunoprecipitation
    • Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody
      Immunoprecipitation of rat brain lysate:
      1. Brain lysate.
      2. Brain lysate immunoprecipitated with Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107), (4 μg).
      3. Brain lysate immunoprecipitated with pre-immune rabbit serum.

      The upper arrow indicates the KCNMA1 channel while the lower arrow indicates the IgG heavy chain.
      Western blot analysis was performed with Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody.
    • Human cardiac fibroblasts (1:750) (Wang, Y.J. et al. (2006) J. Membrane Biol. 213, 175.).
    Immunohistochemistry
    • Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody
      Expression of KCNMA1 in rat penis
      Immunohistochemical staining of rat penis transversal section using Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107). Strong and specific immunostaining is evident in both corpus cavernosum smooth muscle cells (blue arrow) and in the muscular layer of the penis artery (green arrow). Universal Immuno-alkaline-phosphatase Polymer followed by New Fuchsin Subtrate (histofine, Nichirei Corp.) was used for the colour reaction. Hematoxillin is used as the counterstain.
    Immunocytochemistry
    References
    1. Hofland L.J. and Lamberts, S.W.J. (2001) Ann. Oncol. 12, S31.
    2. Fombonne, J. et al. (2003) Rep. Biol. Endocrinol. 1, 19.
    3. Slooter, G.D. et al. (2001) Br. J. Surg. 88, 31.
    4. Schulz, S. et al. (2002) Gynecol. Oncol. 84, 235.
    Scientific background

    KCa1.1 (KCNMA1, BKCa, Maxi K+ or slo) is part of a structurally diverse group of K+ channels that are activated by an increase in intracellular Ca2+. KCa1.1 shows a large single channel conductance when recorded electrophysiologically and hence its name. It differs from the rest of the subfamily members in that it can be activated by both an increase in intracellular Ca2+ and by membrane depolarization. 

    KCa1.1 is expressed in virtually all cell types where it causes hyperpolarization and helps to connect intracellular Ca2+ signaling pathways and membrane excitability.

    Indeed, KCa1.1 channels have a crucial role in smooth muscle contractility, neuronal spike shaping and neurotransmitter release.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-KCNMA1 (KCa1.1) (1184-1200) AntibodyExpression of BKCa in mouse mesenteric cells.A. Immunocytochemical staining of mouse mesenteric smooth muscle cells. Staining of BKCa using Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107) (green) and CaV1.2 (red) in WT (upper) and Caveolin-1 KO (lower) mice. BKCa and CaV1.2 co-localization is shown in yellow (arrowheads). B. Ratio of BKCa and CaV1.2 co-localization particle number to total BKCa particle number in WT (= 8) and KO (= 7) myocytes (*, < 0.05).Adapted from Suzuki, Y. et al. (2013) with permission of the American Society for Biochemistry and Molecular Biology.
    Last update: 24/01/2020

    Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize KCNMA1 from human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Western blot analysis and immunocytochemical staining of rat ROS17/2.8 osteoblasts. Tested in KCNMA1-/- cells.
      Hei, H. et al. (2016) Mol. Cells 39, 530.
    Western blot citations
    1. Mouse podocyte lysate.
      Wang, Y. et al. (2019) Front. Physiol. 10, 167.
    2. Mouse bladder lysate.
      Lu, M. et al. (2018) Am. J. Physiol. 314, C643.
    3. Rat ROS17/2.8 osteoblast lysate. Also tested in KCNMA1-/- cells.
      Hei, H. et al. (2016) Mol. Cells 39, 530.
    4. Human dermal fibroblasts (1:200).
      Kicinska, A. et al. (2016) Biochem. J. 473, 4457.
    5. Mouse colon lysate.
      Bhattarai, Y. et al. (2016) Am. J. Physiol. 311, G210.
    6. Rat VSMC lysate (1:250).
      Chen, M. et al. (2016) Physiol. Rep. 4, e12682.
    7. Mouse kidney lysate.
      Zhang, Y. et al. (2013) Am. J. Physiol. 305, F407.
    8. Mitochondria and mitoplast from human endothelial EA.hy926 cells (1:200).
      Bednarczyk, P. et al. (2013) Am. J. Physiol. 304, H1415.
    Immunoprecipitation citations
    1. Rat VSMC.
      Chen, M. et al. (2016) Physiol. Rep. 4, e12682.
    2. Human CFBE cells.
      Manzanares, D. et al. (2015) J. Biol. Chem. 290, 25710.
    3. Human cardiac fibroblasts (1:750).
      Wang, Y.J. et al. (2006) J. Membrane Biol. 213, 175.
    Immunohistochemistry citations
    1. Mouse kidney sections (1:200).
      Li, Y. et al. (2016) PLoS ONE 11, e0155006.
    2. Human chorionic plate arterial smooth muscle cell sections (10 µg/ml).
      Brereton, M. et al. (2013) PLoS ONE 8, e57451.
    Immunocytochemistry citations
    1. Mouse podocytes.
      Wang, Y. et al. (2019) Front. Physiol. 10, 167.
    2. Rat ROS17/2.8 osteoblasts. Also tested in KCNMA1-/- cells.
      Hei, H. et al. (2016) Mol. Cells 39, 530.
    3. HEK 293 transfected cells (1:500).
      Velazquez-Merrero, C. et al. (2016) J. Neurosci. 36, 10625.
    4. Rat pinealocytes.
      Mizutani, H. et al. (2016) Am. J. Physiol. 310, C740.
    5. Rat hippocampal cells.
      Palacio, S. et al. (2015) Alchol. Clin. Exp. Res. 39, 1619.
    6. Human chorionic plate arterial smooth muscle cells (10 µg/ml).
      Brereton, M. et al. (2013) PLoS ONE 8, e57451.
    7. Mouse mesenteric smooth muscle cells (1:100).
      Suzuki, Y. et al. (2013) J. Biol. Chem. 288, 36750.
    8. E9 chick CG neurons.
      Jha, S. and Dryer, S.T. (2009) FEBS Lett. 583, 3109.
    More product citations
    1. Wan, E. et al. (2013) FASEB J. 27, 1859.
    2. Yang, Y. et al. (2013) J. Physiol. 591, 1277.
    3. Howitt, L. et al. (2012) Am. J. Physiol. 302, H2464.
    4. Liu, Y. et al. (2012) Am. J. Physiol. 302, G44.
    5. Howitt, L. et al. (2011) Am. J. Physiol. 301, H29.
    6. Zuidema, M.Y. et al. (2010) Am. J. Physiol. 299, H1554.
    7. Grimes, W.N. et al. (2009) Nat. Neurosci. 12, 585.
    8. Li, Y. et al. (2009) BMC Dev. Biol. 9, 67.
    9. Yang, Y. et al. (2009) J. Physiol. 587, 3025.
    10. Rodriguez-Vilarrupla, A. et al. (2008) Liver Int. 28, 566.
    11. Ng, Y.K. et al. (2007) Am. J. Physiol. 292, R2100.
    12. Burnham, M. et al. (2006) Am. J. Physiol. 290, H1520.
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