Alternative Name Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1
KO Validated
The specificity of this antibody has been validated in a knockout (KO) or knockdown (KD) system.
Lyophilized Powder Lyophilized Powder
This product is freeze dried. All water molecules have been removed.
Antigen Incl. Antigen Incl.
This antibody is shipped with its antigen FREE of charge!
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)STANRPNRPKSRESRDK, corresponding to amino acid residues 1184-1200 of mouse KCNMA1 (Accession number Q08460). Intracellular, C-terminal part.
Accession (Uniprot) Number Q08460
Gene ID 16531
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical; human, bovine, chicken, dog - 16/17 amino acid residues identical.
RRID AB_2040091.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.6 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Negative control antigen reconstitution 100 µl double distilled water (DDW).
Negative control antigen storage after reconstitution -20°C.
Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, ip, wb
May also work in: ifc*
Western blot
Western blot analysis of rat brain membranes:1. Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107), (1:500).
2. Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody, preincubated with the negative control antigen.
Immunoprecipitation
Immunoprecipitation of rat brain lysate:1. Brain lysate.
2. Brain lysate immunoprecipitated with Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107), (4 μg).
3. Brain lysate immunoprecipitated with pre-immune rabbit serum.
The upper arrow indicates the KCNMA1 channel while the lower arrow indicates the IgG heavy chain.
Western blot analysis was performed with Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody.- Human cardiac fibroblasts (1:750) (Wang, Y.J. et al. (2006) J. Membrane Biol. 213, 175.).
Immunohistochemistry
Expression of KCNMA1 in rat penisImmunohistochemical staining of rat penis transversal section using Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107). Strong and specific immunostaining is evident in both corpus cavernosum smooth muscle cells (blue arrow) and in the muscular layer of the penis artery (green arrow). Universal Immuno-alkaline-phosphatase Polymer followed by New Fuchsin Subtrate (histofine, Nichirei Corp.) was used for the colour reaction. Hematoxillin is used as the counterstain.
Immunocytochemistry
- E9 chick CG neurons (Jha, S. and Dryer, S.T. (2009) FEBS Lett. 583, 3109.).
References
- Hofland L.J. and Lamberts, S.W.J. (2001) Ann. Oncol. 12, S31.
- Fombonne, J. et al. (2003) Rep. Biol. Endocrinol. 1, 19.
- Slooter, G.D. et al. (2001) Br. J. Surg. 88, 31.
- Schulz, S. et al. (2002) Gynecol. Oncol. 84, 235.
Scientific background
KCa1.1 (KCNMA1, BKCa, Maxi K+ or slo) is part of a structurally diverse group of K+ channels that are activated by an increase in intracellular Ca2+. KCa1.1 shows a large single channel conductance when recorded electrophysiologically and hence its name. It differs from the rest of the subfamily members in that it can be activated by both an increase in intracellular Ca2+ and by membrane depolarization.
KCa1.1 is expressed in virtually all cell types where it causes hyperpolarization and helps to connect intracellular Ca2+ signaling pathways and membrane excitability.
Indeed, KCa1.1 channels have a crucial role in smooth muscle contractility, neuronal spike shaping and neurotransmitter release.
Lyophilized PowderNegative Control Antigen Included
Image & Title: 
Expression of BKCa in mouse mesenteric cells.A. Immunocytochemical staining of mouse mesenteric smooth muscle cells. Staining of BKCa using Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107) (green) and CaV1.2 (red) in WT (upper) and Caveolin-1 KO (lower) mice. BKCa and CaV1.2 co-localization is shown in yellow (arrowheads). B. Ratio of BKCa and CaV1.2 co-localization particle number to total BKCa particle number in WT (n = 8) and KO (n = 7) myocytes (*, p < 0.05).Adapted from Suzuki, Y. et al. (2013) with permission of the American Society for Biochemistry and Molecular Biology.
Expression of BKCa in mouse mesenteric cells.A. Immunocytochemical staining of mouse mesenteric smooth muscle cells. Staining of BKCa using Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107) (green) and CaV1.2 (red) in WT (upper) and Caveolin-1 KO (lower) mice. BKCa and CaV1.2 co-localization is shown in yellow (arrowheads). B. Ratio of BKCa and CaV1.2 co-localization particle number to total BKCa particle number in WT (n = 8) and KO (n = 7) myocytes (*, p < 0.05).Adapted from Suzuki, Y. et al. (2013) with permission of the American Society for Biochemistry and Molecular Biology.Last update: 24/01/2020
Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize KCNMA1 from human, rat, and mouse samples.
For research purposes only, not for human use
Applications
Specifications
Scientific Background
Citations
Citations
KO validation citations
- Western blot analysis and immunocytochemical staining of rat ROS17/2.8 osteoblasts. Tested in KCNMA1-/- cells.
Hei, H. et al. (2016) Mol. Cells 39, 530.
Western blot citations
- Mouse podocyte lysate.
Wang, Y. et al. (2019) Front. Physiol. 10, 167. - Mouse bladder lysate.
Lu, M. et al. (2018) Am. J. Physiol. 314, C643. - Rat ROS17/2.8 osteoblast lysate. Also tested in KCNMA1-/- cells.
Hei, H. et al. (2016) Mol. Cells 39, 530. - Human dermal fibroblasts (1:200).
Kicinska, A. et al. (2016) Biochem. J. 473, 4457. - Mouse colon lysate.
Bhattarai, Y. et al. (2016) Am. J. Physiol. 311, G210. - Rat VSMC lysate (1:250).
Chen, M. et al. (2016) Physiol. Rep. 4, e12682. - Mouse kidney lysate.
Zhang, Y. et al. (2013) Am. J. Physiol. 305, F407. - Mitochondria and mitoplast from human endothelial EA.hy926 cells (1:200).
Bednarczyk, P. et al. (2013) Am. J. Physiol. 304, H1415.
Immunoprecipitation citations
- Rat VSMC.
Chen, M. et al. (2016) Physiol. Rep. 4, e12682. - Human CFBE cells.
Manzanares, D. et al. (2015) J. Biol. Chem. 290, 25710. - Human cardiac fibroblasts (1:750).
Wang, Y.J. et al. (2006) J. Membrane Biol. 213, 175.
Immunohistochemistry citations
- Mouse kidney sections (1:200).
Li, Y. et al. (2016) PLoS ONE 11, e0155006. - Human chorionic plate arterial smooth muscle cell sections (10 µg/ml).
Brereton, M. et al. (2013) PLoS ONE 8, e57451.
Immunocytochemistry citations
- Mouse podocytes.
Wang, Y. et al. (2019) Front. Physiol. 10, 167. - Rat ROS17/2.8 osteoblasts. Also tested in KCNMA1-/- cells.
Hei, H. et al. (2016) Mol. Cells 39, 530. - HEK 293 transfected cells (1:500).
Velazquez-Merrero, C. et al. (2016) J. Neurosci. 36, 10625. - Rat pinealocytes.
Mizutani, H. et al. (2016) Am. J. Physiol. 310, C740. - Rat hippocampal cells.
Palacio, S. et al. (2015) Alchol. Clin. Exp. Res. 39, 1619. - Human chorionic plate arterial smooth muscle cells (10 µg/ml).
Brereton, M. et al. (2013) PLoS ONE 8, e57451. - Mouse mesenteric smooth muscle cells (1:100).
Suzuki, Y. et al. (2013) J. Biol. Chem. 288, 36750. - E9 chick CG neurons.
Jha, S. and Dryer, S.T. (2009) FEBS Lett. 583, 3109.
More product citations
- Wan, E. et al. (2013) FASEB J. 27, 1859.
- Yang, Y. et al. (2013) J. Physiol. 591, 1277.
- Howitt, L. et al. (2012) Am. J. Physiol. 302, H2464.
- Liu, Y. et al. (2012) Am. J. Physiol. 302, G44.
- Howitt, L. et al. (2011) Am. J. Physiol. 301, H29.
- Zuidema, M.Y. et al. (2010) Am. J. Physiol. 299, H1554.
- Grimes, W.N. et al. (2009) Nat. Neurosci. 12, 585.
- Li, Y. et al. (2009) BMC Dev. Biol. 9, 67.
- Yang, Y. et al. (2009) J. Physiol. 587, 3025.
- Rodriguez-Vilarrupla, A. et al. (2008) Liver Int. 28, 566.
- Ng, Y.K. et al. (2007) Am. J. Physiol. 292, R2100.
- Burnham, M. et al. (2006) Am. J. Physiol. 290, H1520.
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