Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody

Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1
    Cat #: APC-021
    Alternative Name Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    Immunogen
    • SHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMTRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC, corresponding to residues 1097-1196 of mouse KCNMA1 variant 2 (Accession Q08460-2). Intracellular, C-terminus.
    • Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody
    Accession (Uniprot) Number Q08460-2
    Gene ID 16531
    Peptide confirmation Confirmed by DNA sequence and SDS-PAGE.
    Homology Chicken - 91/99 amino acid residues identical; rabbit - 88/99 amino acid residues identical; turtle - 87/99 amino acid residues identical.
    RRID AB_2313725.
    Purity The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.4 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 μl PBS.
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 3 µg fusion protein per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
    Western blot
    • Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody
      Western blot analysis of rat brain membranes:
      1. Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody (#APC-021), (1:200).
      2. Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody, preincubated with the control antigen.
    Immunoprecipitation
    • Rat brain lysates (2 μg) (Park, S.M. et al. (2004) FEBS Lett. 570, 143.).
    Immunohistochemistry
    • Rat brain sections.

      Mouse vomeronasal sections (Zhang, P. et al. (2008) J. Neurophysiol. 100, 1824.).
    Immunocytochemistry
    • Mouse vomeronasal neurons (Zhang, P. et al. (2008) J. Neurophysiol. 100, 1824.).
    References
    1. Wallner, M. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 4137.
    2. Xia, X.M. et al. (1999) J. Neurosci. 19, 5255.
    3. Orio, P. et al. (2002) News Physiol. Sci. 17, 156.
    Scientific background

    The KCa1.1 channel (also known as KCNMA1, BKCa, Maxi K+ or slo) is part of a structurally diverse group of K+ channels that are activated by an increase in intracellular Ca2+. KCa1.1 shows a large single channel conductance when recorded electrophysiologically and hence its name. It differs from the rest of the subfamily members in that it can be activated by both an increase in intracellular Ca2+ and by membrane depolarization. In addition, the KCa1.1 channel structurally differs from the other Ca2+-dependent K+ channels. While the latter group has a topology that resembles that of the voltage-dependent K+ channels, the KCa1.1 channel has an extracellular N-terminus domain as well as an additional transmembrane domain.

    KCa1.1 is expressed in virtually all cell types where it causes hyperpolarization and helps to connect intracellular Ca2+ signaling pathways and membrane excitability.

    Indeed, KCa1.1 channels play a crucial role in smooth muscle contractility, neuronal spike shaping and neurotransmitter release.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody Expression of KCNMA1 (KCa1.1) in rat VNO sections.Immunohistochemical staining of rat vomeronasal organ (VNO) sections using Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody (#APC-021). KCNMA1 staining (red) is specifically localized to the sensory epithelium with heavy labeling at the apical surface. Adapted from Zhang, P. et al. (2008) J. Neurophysiol. 100, 1824. with permission of The American Physiological Society.
    Last update: 14/04/2020

    Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody (#APC-021) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KCNMA1 from human, mouse, and rat samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Published figures using this product
    • Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody
      Expression of KCa1.1 in mouse SAN cells.
      Immunocytochemical staining of mouse sinoatrial cells (SANCs) using Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody (#APC-021). BK channel expression (red) overlaps with that of HCN4 (green). No detection of KCa1.1 was observed with the secondary antibody.
      Adapted from Lai, M.H. et al. (2014) with permission of the American Physiological Society.
    KO validation citations
    1. Immunostaining of isolated mitochondria from mouse cardiomyocytes. Tested in KCNMA1-/- mice.
      Goswami, S.K. et al. (2019) Front. Cardiovasc. Med. 5, 194.
    Western blot citations
    1. Mouse CCDcl1 cell lysate (1:1000).
      Li, Y. et al. (2016) PLoS ONE 11, e0155006.
    2. Rat brain and colon mucosal scraping lysates.
      Perry, M.D. et al. (2016) Am. J. Physiol. 311, G785.
    3. Mouse bladder lysate (1:1000). Also tested in KCa1.1 knockout mice.
      Zemen, B.G. et al. (2015) Physiol. Rep. 3, e12612.
    4. Mouse aorta lysate (1∶1000).
      Rueda, A. et al. (2013) PLoS ONE 8, e53321.
    5. Rat mesenteric arteries (1:300).
      Shi, L. et al. (2013) Mech. Ageing Dev. 134, 416.
    6. Rat cerebral arteries lysate.
      Xie, M-J. et al. (2010) Am. J. Physiol. 298, C1489.
    7. Rat heart endothelial cell lysate.
      Ying, W-Z. et al. (2009) Hypertension 54, 1159.
    8. HEK293T transfected cell lysates.
      Alioua, A. et al. (2008) J. Biol. Chem. 283, 4808.
    9. Mouse cerebral arteries (1:500).
      Adebiyi, A. et al. (2007) Am. J. Physiol. 292, H1584.
    10. Mouse cardiac tissues (1:300).
      Ambroisine, M-L. et al. (2007) Circulation. 116, 2435.
    11. Rat mesenteric arteries.
      Bolognesi, M. et al. (2007) J. Pharmacol. Exp. Ther. 321, 187.
    12. Mouse pulmonary artery.
      Marino, M. et al. (2007) Am. J. Physiol. 293, L1171.
    13. Rat renal arteries.
      Michel, F.S. et al. (2007) Am. J. Physiol. 293, H1673.
    14. Mouse kidney (1:100).
      Yang, S-S. et al. (2007) Cell Metabol. 5, 331.
    15. Mouse colons.
      Hagen, B.M. and Sanders, K.M. (2006) Am J. Physiol. 291, C750.
    16. Rat aortas.
      Kiyoshi, H. et al. (2006) Am. J. Physiol. 291, H2723.
    17. Mouse forebrain.
      Moriguchi, S. et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 10811.
    18. Rat superior mesenteric artery.
      Bratz, I.N. et al. (2005) Am. J. Physiol. 289, H1277.
    19. Feline circular smooth muscle esophagus (1:200).
      Muinuddin, A. et al. (2005) Am. J. Physiol. 288, G1233.
    20. Rat aorta and mesenteric artery (1:500).
      Xu, H. et al. (2005) Hypertension 46, 1154.
    21. Rat and pig brain.
      Xi, Q. et al. (2004) Am J. Physiol. 286, H610.
    22. Human left internal mammary arteries (LIMAs) and saphenous veins (SVs).
      Archer, S.L. et al. (2003) Circulation 107, 769.
    23. Rat basilar artery.
      Gerzanich, V. et al. (2003) Circ. Res. 93, 805.
    24. Guinea-pig ventricular myocytes.
      Xu, W. et al. (2002) Science 298, 1029.
    25. Rabbit ductus arteriosus.
      Thebaud, B. et al. (2002) Pediatr. Res. 52, 19.
    26. Rat pulmonary artery lysate.
      Reeve, H.L. et al. (2001) J. Appl. Physiol. 90, 2249.
    27. Ovine pulmonary artery.
      Rhodes, M.T. et al. (2001) Am. J. Physiol. 280, L1250.
    28. Mouse uterus smooth muscle lysate.
      Benkusky, N.A. et al. (2000) J. Biol. Chem. 275, 27712.
    Immunoprecipitation citations
    1. Rat brain lysates (2 μg).
      Park, S.M. et al. (2004) FEBS Lett. 570, 143.
    Immunohistochemistry citations
    1. Rat colon sections (1:50).
      Perry, M.D. et al. (2016) Am. J. Physiol. 311, G785.
    2. Mouse cochlear duct (1:500).
      Maison, S.F. et al. (2013) J. Neurophysiol. 109, 1525.
    3. Mouse brain sections (1:750).
      Montgomery, J.R. et al. (2013) Am. J. Physiol. 304, C299.
    4. Rat mesenteric arteries (1:200).
      Shi, L. et al. (2013) Exp. Physiol. 98, 326.
    5. Rat artery sections (1:50).
      Ellis, A. et al. (2009) J. Pharmacol. Exp. Ther. 330, 413.
    6. Mouse organ of Corti inner hair cells.
      Winter, H. et al. (2009) J. Neurosci. 29, 2581.
    7. Rat heart sections.
      Ying, W-Z. et al. (2009) Hypertension 54, 1159.
    8. Mouse vomeronasal sections.
      Zhang, P. et al. (2008) J. Neurophysiol. 100, 1824.
    9. Mouse colonic tissue.
      Flores, C.A. et al. (2007) J. Physiol. 583.2, 705.
    10. Mouse cochleae (1:100).
      Guipponi, M. et al. (2007) Am. J. Pathol. 171, 608.
    11. Human mucosal biopsies from the ascending and sigmoid colon.
      Sandle, G.I. et al. (2007) J. Pathol. 212, 66.
    12. Mouse urinary bladder smooth muscle (UBSM) (1:1000).
      Werner. M.E. et al. (2007) Am. J. Physiol. 292, R616.
    13. Mouse brain (1:500).
      Meredith, A.L. et al. (2006) Nat. Neurosci. 9, 1041.
    14. Ovine pulmonary artery smooth muscle cells (PASMC).
      Resnik, E. et al. (2006) Am. J. Physiol. 290, L761.
    15. Rat brain sections (1:200).
      Benhassine, N. and Berger, T. (2005) Eur. J. of Neurosci. 21, 914.
    16. Mouse penis (1:1000).
      Werner, M.E. et al. (2005) J. Physiol. 567.2, 545.
    17. Rat Vascular smooth muscle cells (VSMCs) from mesenteric artery (MA) (1:400).
      Xu, H. et al. (2005) Hypertension 46, 1154.
    18. Mouse cochleae.
      Ruttiger, L. et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101, 12922.
    19. Human Odontoblasts.
      Allard, B. et al. (2000) J. Biol. Chem. 275, 25556.
    Immunocytochemistry citations
    1. Isolated mitochondria from mouse cardiomyocytes. Also tested in KCNMA1-/- mice.
      Goswami, S.K. et al. (2019) Front. Cardiovasc. Med. 5, 194.
    2. Mouse BV-2 and primary microglia.
      Yang, X. et al. (2019) J. Biol. Chem. 294, 12921.
    3. Mouse CCDcl1 cells (1:100).
      Li, Y. et al. (2016) PLoS ONE 11, e0155006.
    4. HEK 293 transfected cells (1:500).
      Velazquez-Merrero, C. et al. (2016) J. Neurosci. 36, 10625.
    5. Mouse isolated SAN cells.
      Lai, M.H. et al. (2014) Am. J. Physiol. 307, H1327.
    6. Rat cerebral vascular smooth muscle cells (VSMCs).
      Xie, M-J. et al. (2010) Am. J. Physiol. 298, C1489.
    7. Rat T9 glioma cells.
      Hoa, N. et al. (2009) PLoS One 4, e4631
    8. Mouse vomeronasal neurons.
      Zhang, P. et al. (2008) J. Neurophysiol. 100, 1824.
    9. Rat hippocampal neurons (1:250).
      O’Malley, D. et al. (2005) FASEB J. 19, 1917.
    10. Guinea-pigs vas deferens and urinary bladder smooth muscle cells.
      Ohi, Y. et al. (2001) J. Physiol. 534.2, 313.
    Immunofluorescence citations
    1. Mouse BV-2 and primary microglia.
      Yang, X. et al. (2019) J. Biol. Chem. 294, 12921.
    More product citations
    1. Yamashita, M. et al. (2006) FEBS J. 273, 3585.
    2. Pyott, S.J. et al. (2004) J. Neurosci. 24, 9469.
    3. Skinner, L.J. et al. (2003) J. Neurophysiol. 90, 320.
    4. Adamson, C.L. et al (2002) J. Neurosci. 22, 1385.
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