Overview
Cat #:
ACC-124
Alternative Name OTRPC4, VRL-2, VR-OAC, TRP12
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
May also work in: m*
Immunogen
- Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4 (Accession Q9ERZ8). 3rd extracellular loop.
Accession (Uniprot) Number Q9ERZ8
Gene ID 66026
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - 14/16 amino acid residues identical; mouse - 13/16 amino acid residues identical; human - 12/16 amino acid residues identical.
RRID AB_2340915.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, lci, wb
May also work in: ip*
Western blot
- Rat brain lysate (1:200).
- Western blot analysis of rat brain lysate:1. Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:200).
2. Anti-TRPV4 (extracellular) Antibody, preincubated with TRPV4 (extracellular) Blocking Peptide (#BLP-CC124).
Immunohistochemistry
- Rat dorsal root ganglion.
Live cell imaging / Immunocytochemistry
- Human brain glioblastoma (U-87) live cells (1:25-1:50).
Scientific background
TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells1. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65.
TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6.
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Lyophilized Powder
For research purposes only, not for human use
Last Update: 24/09/2024
Specifications
Citations
Citations