This product is freeze dried. All water molecules have been removed.
Every lot is tried & tested in a relevant biological assay.
- Shahidi, S. et al. (1993) Biochim. Biophys. Acta 1157, 74.
- Alomone Labs Apamin-Biotin blocks rat SK2 channels stably transfected in HEK293T cells.A. Time course of Apamin-Biotin (#STA-200-B) action on SK2 channels. Current amplitudes were plotted as a function of time. Membrane potential was held at -80 mV and cells were stimulated by a 150 ms voltage ramp from -120 mV to +60 mV every 10 sec. 1 nM, 10 nM and 50 nM Apamin-Biotin were perfused during 120 sec as indicated by the bar at 0 mV. B. Superimposed examples of SK2 channel current in the absence (control, black) and presence of 1 nM (green), 10 nM (red) and 50 nM (blue) Apamin-Biotin (taken from the experiment in A).Alomone Labs Apamin-Biotin stains rat parietal cortex.Lightly-fixed sections of rat brain were incubated overnight with Apamin-Biotin (#STA-200-B) (1 nM) followed by 45 minutes fixation in cold 4% paraformaldehyde and followed by the addition of streptavidin labeled with ATTO-594. Most of the staining is seen in pyramidal neurons (horizontal arrows) as well as in astrocyte-like processes (vertical arrows).
- 1. Gauldie, J. et al. (1976) Eur. J. Biochem. 61, 369.
- 2. Logsdon, N.J. et al. (1997) J. Biol. Chem. 272, 32723.
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- 5. Barfod, E.T. et al. (2001) Am. J. Physiol. 280, C836.
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Apamin is a natural peptide isolated and purified from Apis mellifera bee venom. Apamin blocks small conductance Ca2+-activated K+ channels (SK). It is specific for the SK1-3 isoforms, but ineffective in blocking other calcium activated K+ channels2. In mammalian cell lines, Apamin blocks expressed hSK1 with an IC50 of ~10 nM3, rSK1 and rSK2 with IC50 of ~3 and <1 nM respectively4 and liver rSK3 with IC50<1 nM.5 In Jurkat T-cells, 5 nM of Apamin blocks 70% of hSK2 currents.6
Blocking these channels with Apamin slows down neuronal activity after hyperpolarization7, as well as smooth muscle contraction8 and catecholamine secretion in adrenal glands.9 Electrophysiology application of long (~400 ms) voltage ramps, covering the whole physiological range (-160 to +40 mV), in the whole cell patch-clamp or voltage clamp configurations, before and after bath perfusion of the drug, allows the detection of the blocked channel currents (by comparison).1
Apamin-Biotin (#STA-200-B) is a highly pure, synthetic, and biologically active conjugated peptide toxin.
We gladly take on collaboration projects. Please Contact Us.
- Anti-KCNN1 (KCa2.1, SK1) Antibody (#APC-039)
- Anti-KCNN2 (KCa2.2, SK2) Antibody (#APC-028)
- Guinea pig Anti-KCNN2 (KCa2.2, SK2) Antibody (#AGP-045)
- Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody (#APC-025)
- Anti-KCNN3 (KCa2.3, SK3) (N-term)-ATTO-594 Antibody (#APC-025-AR)
- Anti-KCNN3 (KCa2.3, SK3) (C-term) Antibody (#APC-103)