Name: ω-Conotoxin Bu8
Brand: Alomone Labs
SKU: STC-180

Overview

Cat #: STC-180

Alternative Name Bu8, Omega-conotoxin Bu8

Lyophilized Powder yes

Origin Conus bullatus (Bubble cone)

Source Synthetic peptide

MW: 2753 Da

Purity: >98%

Form Lyophilized Powder

Effective concentration 10 – 100 nM

Sequence CKRKGSSCRRTSYDCCTGSCRNGKC – NH₂

Modifications Disulfide bonds location- Cys¹-Cys¹⁶, Cys⁸-Cys²⁰, Cys¹⁵-Cys²⁵
Cys²⁵ – C-terminal amidation

Structure

Molecular formula C₁₀₃H₁₇₄N₄₂O₃₅S₆

Activity Specific and potent N-type voltage-gated calcium (Ca_V2.2) channel blocker¹.

References-Activity

Chen, J. et al. (2021) Acta Pharm. Sin. B., 11, 2685.

Accession number P0CY66

Shipping and storage The product is shipped as a lyophilized powder at room temperature. Upon receipt, it should be stored at -20°C. Protect from moisture.

Solubility Soluble in water. For long-term storage in solution, we recommend to prepare a stock solution by dissolving the product in ddH2O at a concentration X100-1000 of final working solution. Divide the solution into small aliquots and store at -20°C. Upon use, thaw the relevant vial intended for use and dilute in your desired working buffer. The preparation of fresh solutions in working buffers before use is recommended. Repeat freeze-thawing might result in loss of activity. Centrifuge all product preparations before use (10000 x g, 5 min).

Storage of solutions The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the toxin in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Our bioassay

Alomone Labs ω-Conotoxin Bu8 inhibits Ca_V2.2 channel currents heterologously expressed in Xenopus oocytes.
A. Representative time course of ω-Conotoxin Bu8 (#STC-180) inhibition of Ca_V2.2 (α1B + α2δ1 + β1) channels current. Membrane potential was held at -100 mV, current was elicited by a 100 ms voltage ramp to +60 mV every 10 sec, and inhibited by 10 nM ω-Conotoxin Bu8 (green).
B. Superimposed traces of Ca_V2.2 currents after application of control (black) and of 10 nM ω-Conotoxin Bu8 (green), taken from the recording in A.

References - Scientific background

Chen, J. et al. (2021) Acta Pharm. Sin. B., 11, 2685.
Adams, D.J. and Berecki, G. (2013) Biochim. Biophys. Acta. 1828, 1619.

Scientific background

ω-Conotoxin Bu8 (Bu8) is a 25 amino acid peptidyl toxin that was synthesized from the venom of the cone snail, Conus bullatus¹. This toxin potently and selectively blocks N-type voltage-gated calcium (Ca_v2.2) channels. Bu8 displays higher or similar analgesic activity in the pain models compared to MVIIA but has less adverse effects¹.

Bu8 adopts a canonical globular scaffold, demonstrated as an inhibitory cysteine knot motif, marked by 3 disulfide bonds that is consistent for all ω-conotoxins in the all-crossing pattern of 1-4, 2-5, and 3-6¹.

Ca_v2.2 are predominantly expressed in nerve terminals and are involved in the regulation of neuronal excitability and nociceptive signaling. These channels are multifunctional and play important roles in the transduction of acute and chronic pain perception. They are able to transduce electrical activity into other cellular functions, regulate calcium homeostasis, and process pain information. Since ω-conotoxins selectively inhibit Ca_v2.2 in nociceptors, they are considered attractive molecules for drug design².

Target Ca_V2.2 Ca²⁺ channel

Peptide Content: 100%

Lyophilized Powder

Omega-conotoxin Bu8 (#STC-180) is a highly pure, synthetic, and biologically active peptide toxin.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 02/05/2024

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ω-Conotoxin Bu8

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Scientific Background

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