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µ-Conotoxin PIIIA

μ-Conotoxin P3.7
A Potent Blocker of NaV1.2, NaV1.4 and NaV1.7 Na+ Channels
    Cat #: STC-400
    Alternative Name μ-Conotoxin P3.7
  • Lyophilized Powder
  • Bioassay Tested
    • Origin Synthetic peptide
    MW: 2604 Da.
    Purity: >98% (HPLC)
    Effective concentration 10 nM - 1 μM.
    Sequence QRLCCGFPKSCRSRQCKPHRCC.
    Modifications Disulfide bonds between Cys4-Cys16, Cys5-Cys21, and Cys11-Cys22. Cys22 - C-terminal amidation, Hydroxyprolines at Pro8 and Pro18 and Pyrrolidone carboxylic acid at Q1.
      • µ-Conotoxin PIIIA
    Molecular formula C103H165N40O28S6.
    Activity μ-Conotoxin PIIIA reversibly blocks mammalian skeletal muscle voltage-gated Na+ channels (NaV1.4, IC50 of 44 nM), Type II (NaV1.2) and PN1 (NaV1.7) neuronal voltage-gated Na+ channels (IC50 of 640 nM) by binding to site I domain of the channel. In addition, it blocks irreversibly amphibian muscle Na+ channels1.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • µ-Conotoxin PIIIA
        Alomone Labs μ-Conotoxin PIIIA inhibits NaV1.4 channel currents expressed in Xenopus oocytes.
        NaV1.4 currents were elicited by application of voltage ramp of 60 ms from holding potential of -100 mV to +40 mV. A. Time course of peak current amplitude before (black) and during (green) application of 20 nM μ-Conotoxin PIIIA (#STC-400) applied for 180 s. B. Superimposed example current traces before (black) and during (green) application of the toxin, taken from the experiment in A.
    References - Scientific background
    • 1. Shon, K.J. et al. (1998) J. Neuroscience 18, 4473.
    • 2. Nielsen, K.J. et al. (2002) J. Biol. Chem. 277, 27247.
    • 3. Safo, P. et al. (2000) J. Neurosci. 20, 76.
      • µ-Conotoxin PIIIA is a 22 amino acid peptidyl toxin isolated from the Conus purpurascens (Purple cone) venom and belongs to the conotoxin M superfamily1. It reversibly blocks mammalian skeletal muscle voltage-gated Na+ channels (NaV1.4, IC50 of 44 nM), Type II (NaV1.2) and PN1 (NaV1.7) neuronal voltage-gated Na+ channels (IC50 of 640 nM) by binding to site I domain of the channel1-3. In addition, it irreversibly blocks amphibian muscle Na+ channels1.
    Target Various NaV channels
    Net Peptide Content: 100%
    Lyophilized Powder
    receive a 50 µg free trial sample!
    Last update: 24/01/2020

    µ-Conotoxin PIIIA (#STC-400) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use
    Citations
      • Feng, B. et al. (2015) J. Neurophysiol. 113, 2618.
      • Kirchhof, P. et al. (2015) Circ. Heart Fail. 8, 79.
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