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µ-Conotoxin PIIIA

μ-Conotoxin P3.7

A Potent Blocker of NaV1.2, NaV1.4 and NaV1.7 Na+ Channels

Cat #: STC-400
Alternative Name μ-Conotoxin P3.7
Lyophilized Powder yes
  • Bioassay Tested
    • Origin Synthetic peptide
    MW: 2604 Da
    Purity: >98% (HPLC)
    Effective concentration 10 nM - 1 μM.
    Sequence QRLCCGFPKSCRSRQCKPHRCC.
    Modifications Disulfide bonds between Cys4-Cys16, Cys5-Cys21, and Cys11-Cys22. Cys22 - C-terminal amidation, Hydroxyprolines at Pro8 and Pro18 and Pyrrolidone carboxylic acid at Q1.
    Structure
    Molecular formula C103H165N40O28S6.
    Activity μ-Conotoxin PIIIA reversibly blocks mammalian skeletal muscle voltage-gated Na+ channels (NaV1.4, IC50 of 44 nM), Type II (NaV1.2) and PN1 (NaV1.7) neuronal voltage-gated Na+ channels (IC50 of 640 nM) by binding to site I domain of the channel. In addition, it blocks irreversibly amphibian muscle Na+ channels1.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs μ-Conotoxin PIIIA inhibits NaV1.4 channel currents expressed in Xenopus oocytes.
      Alomone Labs μ-Conotoxin PIIIA inhibits NaV1.4 channel currents expressed in Xenopus oocytes.
      NaV1.4 currents were elicited by application of voltage ramp of 60 ms from holding potential of -100 mV to +40 mV. A. Time course of peak current amplitude before (black) and during (green) application of 20 nM μ-Conotoxin PIIIA (#STC-400) applied for 180 s. B. Superimposed example current traces before (black) and during (green) application of the toxin, taken from the experiment in A.
    References - Scientific background
    1. Shon, K.J. et al. (1998) J. Neuroscience 18, 4473.
    2. Nielsen, K.J. et al. (2002) J. Biol. Chem. 277, 27247.
    3. Safo, P. et al. (2000) J. Neurosci. 20, 76.
    Scientific background µ-Conotoxin PIIIA is a 22 amino acid peptidyl toxin isolated from the Conus purpurascens (Purple cone) venom and belongs to the conotoxin M superfamily1. It reversibly blocks mammalian skeletal muscle voltage-gated Na+ channels (NaV1.4, IC50 of 44 nM), Type II (NaV1.2) and PN1 (NaV1.7) neuronal voltage-gated Na+ channels (IC50 of 640 nM) by binding to site I domain of the channel1-3. In addition, it irreversibly blocks amphibian muscle Na+ channels1.
    Target Various NaV channels
    Peptide Content: 100%
    Lyophilized Powder
    Last update: 06/12/2022

    µ-Conotoxin PIIIA (#STC-400) is a highly pure, synthetic, and biologically active peptide toxin.

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    For research purposes only, not for human use
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