Overview
- Peptide (C)DVIEKRKFNHNSN, corresponding to amino acid residues 168-180 of rat 5-Hydroxytryptamine receptor 4 (Accession Q62758). 2nd extracellular loop.
Cell surface detection of 5HT4 receptor in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody (#ASR-036-F), (2.5 µg).
Cell surface detection of 5HT4 receptor in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody (#ASR-036-F), (5 µg).
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5-hydroxytryptamine receptor 4 (5-HT4 receptor) is a subclass among the large family of receptors mediating serotonin signaling at central and peripheral targets. 5-HT4 receptor activation plays a role in learning and memory, feeding control and stress response. 5-HT4 receptors are encoded by a complex gene which in humans, generates ten different splice variants1, 2.
5-HT4 receptor is a member of the seven transmembrane spanning G-protein-coupled receptors (GPCRs) and couples via Gs to increase cAMP production. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs); the N-terminus is on the extracellular side of the membrane, and the C-terminus is cytoplasmic3.
Within the central nervous system, these receptors are expressed in limbic structures including: hypothalamus, nucleus accumbens, prefrontal cortex, amygdala and hippocampus, where they contribute to emotional and cognitive functions4.
Low levels of 5-HT4 receptors are associated with numerous neuropsychiatric disorders including Alzheimer's disease, Huntington’s disease, feeding disorders and stress-related disorders such as anxiety and depression5.
Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (#ASR-036) is a highly specific antibody directed against an epitope of the rat Serotonin receptor 4. The antibody can be used in western blot, immunocytochemistry, and live cell imaging applications. It has been designed to recognize 5HT4 receptor from rat, mouse, and human samples.
Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody (#ASR-036-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.
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