Anti-5HT4 Receptor (extracellular)-FITC Antibody

5-Hydroxytryptamine (Serotonin) receptor 4, Serotonin receptor 4, HTR4
  • New
Cat #: ASR-036-F
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)DVIEKRKFNHNSN, corresponding to amino acid residues 168-180 of rat 5-hydroxytryptamine receptor 4 (Accession Q62758). 2nd extracellular loop.
    Accession (Uniprot) Number Q62758
    Gene ID 25324
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 12/13 amino acid residues identical; human – 11/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ASR-036), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic, lci
    Direct flow cytometry
    Direct flow cytometry of live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-5HT4 Receptor (extracellular)-FITC Antibody (#ASR-036-F), (2.5 µg).
    Direct flow cytometry of live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-5HT4 Receptor (extracellular)-FITC Antibody (#ASR-036-F), (5 µg).
    References
    1. Mnie-Filali, O. et al. (2010) Cell. Signal. 22, 501.
    2. Barnes, N.M. and Sharp, T. (1999) Neuropharmacology 38, 1083.
    3. Oh, D.Y. et al. (2006) Int. Rev. Cytol. 252, 163.
    4. Bockaert, J. et al. (2004) Curr. Drug Targets CNS Neurol. Disord. 3, 39.
    5. Varnas, K. et al. (2003) Eur. Neuropsychopharmacol. 13, 228.
    Scientific background

    5-hydroxytryptamine receptor 4 (5-HT4 receptor) is a subclass among the large family of receptors mediating serotonin signaling at central and peripheral targets. 5-HT4 receptor activation plays a role in learning and memory, feeding control and stress response. 5-HT4 receptors are encoded by a complex gene which in humans, generates ten different splice variants1, 2.

    5-HT4 receptor is a member of the seven transmembrane spanning G-protein-coupled receptors (GPCRs) and couples via Gs to increase cAMP production. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs); the N-terminus is on the extracellular side of the membrane, and the C-terminus is cytoplasmic3.

    Within the central nervous system, these receptors are expressed in limbic structures including: hypothalamus, nucleus accumbens, prefrontal cortex, amygdala and hippocampus, where they contribute to emotional and cognitive functions4.

    Low levels of 5-HT4 receptors are associated with numerous neuropsychiatric disorders including Alzheimer's disease, Huntington’s disease, feeding disorders and stress-related disorders such as anxiety and depression5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 22/10/2018

    Anti-5HT4 Receptor (extracellular) Antibody (#ASR-036) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and live cell imaging applications.  It has been designed to recognize 5-hydroxytryptamine receptor 4 from rat, mouse, and human samples.

    Anti-5HT4 Receptor (extracellular)-FITC Antibody (#ASR-036-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use
    Citations
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