Free shipping starts now, no minimum, no coupons required!

Anti-Adenosine A1 Receptor (extracellular) Antibody

Adenosine Receptor A1, Adenosine A1R, ADORA1

Cat #: AAR-009
Alternative Name Adenosine Receptor A1, Adenosine A1R, ADORA1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)EQDWRANGSVGEPVIK, corresponding to amino acid residues 153 - 168 of rat Adenosine A1 Receptor (Accession P25099). Extracellular, 2nd loop.
Accession (Uniprot) Number P25099
Gene ID 29290
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 14 out of 16 amino acid residues identical. Not recommended for human samples.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Adenosine A1 Receptor (extracellular) Blocking Peptide (#BLP-AR009)
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1-2. Anti-Adenosine A1 Receptor (extracellular) Antibody (#AAR-009), (1:400).
    3-4. Anti-Adenosine A1 Receptor (extracellular) Antibody, preincubated with Adenosine A1 Receptor (extracellular) Blocking Peptide (BLP-AR009).
  • Western blot analysis of mouse J774 macrophage cell line lysate (lanes 1 and 3) and mouse BV-2 microglia cell line lysate (lanes 2 and 4): 
    Western blot analysis of mouse J774 macrophage cell line lysate (lanes 1 and 3) and mouse BV-2 microglia cell line lysate (lanes 2 and 4): 
    1-2. Anti-Adenosine A1 Receptor (extracellular) Antibody (#AAR-009), (1:200).
    3-4. Anti-Adenosine A1 Receptor (extracellular) Antibody, preincubated with Adenosine A1 Receptor (extracellular) Blocking Peptide (BLP-AR009).
Immunohistochemistry
  • Expression of Adenosine A1 Receptor in rat subventricular zone (SVZ).
    Expression of Adenosine A1 Receptor in rat subventricular zone (SVZ).
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Adenosine A1 Receptor (extracellular) Antibody (#AAR-009), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Adenosine A1 Receptor immunoreactivity (green) appears in the SVZ. B. Pre-incubation of the antibody with Adenosine A1 Receptor (extracellular) Blocking Peptide (BLP-AR009), suppressed staining. Cell nuclei are stained with DAPI (blue). CC = corpus callosum, SVZ = subventricular zone, LV = lateral ventricle.
Indirect flow cytometry
  • Cell surface detection of Adenosine A1 Receptor by indirect flow cytometry in live intact mouse BV-2 microglia cell line:
    Cell surface detection of Adenosine A1 Receptor by indirect flow cytometry in live intact mouse BV-2 microglia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC .
    ___ Cells + Anti-Adenosine A1 Receptor (extracellular) Antibody (#AAR-009), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Okusa, M.D. (2002) Am. J. Physiol. 282, F10.
  2. Nakata, H. (1989) J. Biol. Chem. 264, 16545.
  3. Linden, J. (1991) FASEB J. 5, 2668.
  4. Park, K.S. et al. (2001) J. Pharmacol. Exp. Therap. 299, 501.
  5. Rice, A.M. et al. (2000) Endocrinology 141, 1442.
  6. LaNoue, K.F. and Martin, L.F. (1994) FASEB J. 8, 72.
  7. Synowitz, M. et al. (2006) Cancer Res. 66, 8550.
  8. Lelievre, V. et al. (1998) Eur. J. Pharmacol. 341, 289.
  9. Khoo, H.E. et al. (1996) Cancer Lett. 106, 17.
Scientific background

Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop that is important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs.1-3

A1AR is widely distributed and has been well characterized. High expression of A1AR is found in the brain (mainly in the cortex, cerebellum, and hippocampus), dorsal horn of the spinal cord, adrenal gland, and atria, and to a lower extent in several other tissues including adipose tissue, the colon, and kidney.2,4

A1AR modulates the activity of several ion channels. Activation of A1AR (by adenosine, its major agonist) inhibits N-type Ca2+ channels via a voltage-dependent, pertussis toxin (PTX)-sensitive pathway in neurons of the rat major pelvic ganglia (MPG).5

Since A1AR is the most prominent adenosine receptor in adipocytes, it has become a natural target for research on obesity, which is a major health problem.6,7 A possible role in cell proliferation and carcinogenesis has also been suggested for A1AR.8-10

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/01/2023

Anti-Adenosine A1 Receptor (extracellular) Antibody (#AAR-009) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Adenosine A1 Receptor from mouse and rat samples.
This antibody is not recommended for human samples.

For research purposes only, not for human use
Shipping and Ordering information