Anti-CACNB3 Antibody

CaVβ3, CAB3, CACNLB3, Voltage-dependent L-type calcium channel subunit beta-3
    Cat #: ACC-008
    Alternative Name CaVβ3, CAB3, CACNLB3, Voltage-dependent L-type calcium channel subunit beta-3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)DRNWQRNRPWPKDSY, corresponding to amino acid residues 463-477 of rabbit CACNB3 (Accession P54286). Intracellular, C-terminus.
    • Anti-CACNB3 Antibody
    Accession (Uniprot) Number P54286
    Gene ID 100009402
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse, human, Xenopus - identical; bovine - 14/15 amino acid residues identical.
    RRID AB_2039787.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Specificity Identical for all splice forms.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.3 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-CACNB3 Antibody
      Western blot analysis of rat brain membranes:
      1. Anti-CACNB3 Antibody (#ACC-008), (1:200).
      2. Anti-CACNB3 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    • Anti-CACNB3 Antibody
      Expression of CACNB3 (CaVβ3) in rat hippocampus
      Immunohistochemical staining of rat hippocampus with Anti-CACNB3 Antibody (#ACC-008). A. CaVβ3 (red) appears in neurons (arrows). B. Staining of nerve cells with mouse anti-parvalbumin demonstrates the restriction of CaVβ3 to cell bodies. C. Merged image of panels A and B.
    Immunocytochemistry
    • A7r5 cell line, derived from the rat thoracic aorta (1:400) (Uchida, R. et al. (2003) Eur. J. Pharmacol. 466, 53.).
    References
    1. Arikkath, J. and Campbell, K.P. (2003) Curr. Opin. Neurobiol. 13, 298.
    2. Dolphin, A.C. (2003) J. Bioenerg. Biomembr. 35, 599.
    3. Richards, M.W. et al. (2004) Trends Pharmacol. Sci. 25, 626.
    Scientific background

    The β3 protein is an auxiliary subunit of the CaV (voltage-dependent calcium) channel multimer complex. Four β isoforms were identified in mammalian genomes and they share some sequence as well as structure homology.1-3  The structure of CaVβ3 was recently determined (as well as other CaVβ subunits; for review see reference 3). CaVβ subunits are cytoplasmic proteins, that bind the cytoplasmic face of the pore forming CaV α1 subunits. These interactions have profound effects on channel trafficking to the plasma membrane as well as on current parameters such as voltage-dependent kinetics and interactions with modulatory proteins.1,2  CaVβ3 might interact with all the pore-forming subunits that have been tested and in vivo is expressed in smooth muscle, trachea, aorta, lung and brain.1

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rabbit CaVβ3. Anti-CACNB3 Antibody (#ACC-008) can be used in western blot, immunohistochemistry and immunocytochemistry applications. The antibody has been designed to recognize CaVβ3 from rat, mouse and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Transfected HEK 293 cell lysate (1:5000).
      Bourdin, B. et al. (2015) J. Biol. Chem. 290, 2854.
    Immunocytochemistry citations
    1. A7r5 cell line, derived from the rat thoracic aorta (1:400).
      Uchida, R. et al. (2003) Eur. J. Pharmacol. 466, 53.
    More product citations
    1. Li, L. et al. (2012) J. Biol. Chem. 287, 6002.
    2. Dilger, E.K. et al. (2011) J. Physiol. 589.4, 919.
    3. Thompson, W.R. et al. (2011) J. Bone Min. Res. 26, 2125.
    Shipping and Ordering information