Anti-Stargazin Antibody

CaVγ2, Voltage-dependent calcium channel gamma-2 subunit, CACNG2, TARP gamma-2, Transmembrane AMPAR regulatory protein gamma-2, Waggler
    Cat #: ACC-012
    Alternative Name CaVγ2, Voltage-dependent calcium channel gamma-2 subunit, CACNG2, TARP gamma-2, Transmembrane AMPAR regulatory protein gamma-2, Waggler
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RATDYLQASAITRIPS, corresponding to amino acid residues 213-228 of mouse CaVγ2 (Accession O88602). Intracellular, C-terminus.
        Anti-Stargazin Antibody
    Accession (Uniprot) Number O88602
    Gene ID 12300
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human - identical.
    RRID AB_2039789.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.6 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, wb
    May also work in: ifc*, ih*, ip*
      • Anti-Stargazin Antibody
        Western blot analysis of rat brain membranes:
        1. Anti-Stargazin Antibody (#ACC-012), (1:200).
        2. Anti-Stargazin Antibody, preincubated with the negative control antigen.
      • Rat spinal cord culture (1:200) (Krishnamurthy, K. et al. (2013) Brain Res. 1529, 143.).
    References
    • 1. Arikkath, J. et al. (2003) Curr. Opin. Neurobiol. 13, 298.
    • 2. Chen, L. et al. (2000) Nature 408, 936.
      • The γ subunit protein is an auxiliary subunit of the CaV (voltage-dependent calcium) channel multimer complex. γ1 was first identified in skeletal muscle as a part of the proteins complex purified as the DHP receptor. Eight γ subunit isoforms were identified in mammalian genomes and they share some sequence as well as structure homology.γ subunit contains four transmembrane helices, with intracellular N- and C- termini. Usually their association with the pore-forming α1 subunit results in reduced current amplitude.

        CaVγ2 is also known as Stargazin, and interacts and regulates the activity and trafficking of AMPA glutamate receptors.2

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat CaVγ2. Anti-Stargazin Antibody (#ACC-012) can be used in western blot analysis. It has been designed to recognize CaVγ2 from rat, mouse and human samples.

    For research purposes only, not for human use
    Citations
      • Rat spinal cord culture (1:200).
        Krishnamurthy, K. et al. (2013) Brain Res. 1529, 143.
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