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Anti-CACNG8 Antibody

CaVγ8, Voltage-dependent calcium channel gamma-8 subunit, TARP gamma-8, Transmembrane AMPAR regulatory protein gamma-8

Cat #: ACC-125
Alternative Name CaVγ8, Voltage-dependent calcium channel gamma-8 subunit, TARP gamma-8, Transmembrane AMPAR regulatory protein gamma-8
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)ESLKRWNEERGLW(S)EK, corresponding to amino acid residues 2-17 of rat CACNG8 (Accession Q8VHW5). Intracellular, N-terminus.
Accession (Uniprot) Number Q8VHW5
Gene ID 140729
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse, human - 15/16 amino acid residues identical.
RRID AB_2756551.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat testis (lanes 1 and 4), rat brain (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:
    Western blot analysis of rat testis (lanes 1 and 4), rat brain (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:
    1-3. Anti-CACNG8 Antibody (#ACC-125), (1:400).
    4-6. Anti-CACNG8 Antibody, preincubated with CACNG8 Blocking Peptide (#BLP-CC125).
Immunohistochemistry
  • Expression of TARP γ8 (CACNG8) in rat hippocampus
    Expression of TARP γ8 (CACNG8) in rat hippocampus
    Immunohistochemical staining of rat hippocampus using Anti-CACNG8 Antibody (#ACC-125). A. TARP γ8 staining (green) appears in neuronal soma (horizontal arrows) and in the neuronal processes (diagonal arrows). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.
References
  1. Cho, C.H. et al. (2007) Neuron 55, 890.
  2. Milstein, A.D. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 11348.
  3. Hamad, M.I. et al. (2014) Development 141, 1737.
  4. Tomita, S. et al. (2003) J. Cell Biol. 161, 805.
  5. Fukaya, M. et al. (2006) Eur. J. Neurosci. 24, 2177.
  6. Kato, A.S. et al. (2010) Neuron 68,1082.
Scientific background

Transmembrane AMPA receptor regulatory proteins (TARPs) serve as auxiliary subunits of AMPA receptors that regulate functional aspects of these receptors such as: fast excitatory synaptic transmission, surface trafficking, enhancing synaptic clustering and increasing glutamate affinity. Generally, TARPs are responsible for regulating expression, channel properties and localization of AMPA receptors in the brain1.

TARPs are non-pore-forming integral membrane proteins with four transmembrane domains that are widely expressed in the CNS. 

The TARP family is divided in Type I and type II subfamily. Type I TARPs comprise four calcium channel γ subunits: γ2, γ3, γ4, and γ-8, also known as Cacng2, 3, 4 and 8, respectively2,3.

γ-8 TARP predominantly expressed in the telencephalon with abundant distribution on hippocampal excitatory synapses and extrasynaptic membranes. It is expressed in low levels in newborn and neonatal brain and can be found in higher levels in adult brain4. γ-8 plays an important role in increasing the number of synaptic and extrasynaptic AMPA receptors on dendrites and spines5

The C terminus of γ-8 contains 3 unique stretches of amino acids that are not found in any of the other TARPs. In addition, it contains PDZ-binding motifs2. In heterologous cells, γ-8 prolong the current rise time in response to small amounts of glutamate and slow GluA1 receptor desensitization and deactivation3.

Several studies have shown that overexpression of TARP γ-8 increases the number of AMPARs in the plasma membrane. In γ-8 knockout mice, AMPA receptor expression is severely reduced from the synaptic and extrasynaptic membranes of dendrites and does not progress through the secretory pathway5,6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat TARP gamma-8 (CaVγ8, CACNG8). Anti-CACNG8 Antibody (#ACC-125) can be used in western blot and immunohistochemistry applications. It has been designed to recognize TARP gamma-8 from rat, mouse and human samples.

For research purposes only, not for human use
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