Anti-Connexin-36-ATTO-488 Antibody

CX36, Gap junction δ2 protein, Gap junction α9 protein, GJD2, GJA9
Cat #: ACC-209-AG
Alternative Name CX36, Gap junction δ2 protein, Gap junction α9 protein, GJD2, GJA9
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: r
    May also work in: h, m
      • Peptide (C)EVKELAPHPSGLR, corresponding to amino acid residues 170-182 of rat Connexin-36 (Accession O70610). Intracellular loop.
    Accession (Uniprot) Number O70610
    Gene ID 50564
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - 12/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-209), and immunohistochemical staining (labeled antibody).
    Applications: ih
    May also work in: ic
      • Expression of Connexin-36 in rat spinal cord
        Immunohistochemical staining of Connexin-36 in rat spinal cord frozen sections using Anti-Connexin-36-ATTO-488 Antibody (#ACC-209-AG). A. Cx36 (green) immunoreactive grains scattered along layer I of the dorsal horn (arrows). B. Nuclei were demonstrated using DAPI (blue) as the counterstain. C. Merged image of panel A and panel B.
      • The structural unit of the gap junctions is the connexon. Connexons are hexameric structures, formed by transcellular channels composed of connexins. Connexins (Cx) share a common structural basis, which is a four-transmembrane spanning protein, a cytoplasmic loop, two extracellular loops (E1–E2), one intracellular amino-(NT domain) and a carboxy-terminal (CT domain) region1.

        Connexin 36 (Cx36) is predominantly expressed in mammalian neuronal cells2. Cx36 is expressed in a variety of mammalian brain regions, but expression is particularly notable in the inferior olive, cerebellar cortex, olfactory bulbs, several brainstem nuclei, the CA3 subregion of the hippocampus and reticular thalamic nuclei3. Outside of the central nervous system, Cx36 also appears in motoneurons of adult rat spinal cord 4.

        Studies have shown that Cx36 is involved in some neural functions, including neuronal neurogenesis, neural fertility control and regulation of waking and sleep states, and also Cx36 plays an important role in N-methyl-D-aspartate-mediated neuronal death following neuronal injury5. A number of studies performed in humans and animals have confirmed the role of Cx36 in the generation and maintenance of seizure activity6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 06/05/2019

    Anti-Connexin-36 Antibody (#ACC-209) is a highly specific antibody targeted against an epiotpe of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Cx36 from human, rat, and mouse samples.

    Anti-Connexin-36-ATTO-488 Antibody (#ACC-209-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well-known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Connexin-36-ATTO-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use