Anti-Connexin-47 (GJC2) Antibody

CX47, Gap Junction Gamma-2 Protein, Gap Junction Alpha-12 Protein, GJA12, Connexin-46.6

Cat #: ACC-222
Alternative Name CX47, Gap Junction Gamma-2 Protein, Gap Junction Alpha-12 Protein, GJA12, Connexin-46.6
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)HRLARASEQERR, corresponding to amino acid residues 100 - 111 of rat Connexin-47 (Accession Q80XF7). Intracellular loop.
Accession (Uniprot) Number Q9EP66
Gene ID 80885
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human -  identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1-2. Anti-Connexin-47 (GJC2) Antibody (#ACC-222), (1:200).
    3-4. Anti-Connexin-47 (GJC2) Antibody, preincubated with Connexin-47 (GJC2) Blocking Peptide (BLP-CC222).
  • Western blot analysis of human U-87 MG glioblastoma cell line lysate:
    Western blot analysis of human U-87 MG glioblastoma cell line lysate:
    1. Anti-Connexin-47 (GJC2) Antibody (#ACC-222), (1:200).
    2. Anti-Connexin-47 (GJC2) Antibody, preincubated with Connexin-47 (GJC2) Blocking Peptide (BLP-CC222).
Immunohistochemistry
  • Expression of Connexin-47 in rat spinal cord ventral horn region.
    Expression of Connexin-47 in rat spinal cord ventral horn region.
    Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-Connexin-47 (GJC2) Antibody (#ACC-222), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the ventral horn region showed CX-47 immunoreactivity (green) in neuron profiles (arrows). B. Pre-incubation of the antibody with Connexin-47 (GJC2) Blocking Peptide (BLP-CC222), suppressed staining. Cell nuclei are stained with DAPI (blue).
  • Expression of Connexin-47 in rat spinal cord dorsal horn region.
    Expression of Connexin-47 in rat spinal cord dorsal horn region.
    Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-Connexin-47 (GJC2) Antibody (#ACC-222), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CX-47 immunoreactivity (green) appears in the first layers of the dorsal horn (vertical arrows) and in neuron profiles (horizontal arrows). B. Pre-incubation of the antibody with Connexin-47 (GJC2) Blocking Peptide (BLP-CC222), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Nielsen, M.S. et al (2012), Compr. Physiol., 2, 1981.
  2. Basu and Sarma (2018), J Biosci, 43, 1055.
  3. Fasciani et al (2018), Glia, 66, 2340.
  4. Zhao, Y. et al (2020) Proc Natl Acad Sci U S A, 117, 2160
Scientific background

Connexin-47 (Cx47) is a gap junction (GJ) protein expressed in the Central nervous system.

It belongs to the connexins family of transmembrane proteins. Proteins that belong to this family form intercellular membrane channels that allow diffusional movement of ions, metabolites, and signaling molecules. The family members are composed of four transmembrane segments, an unstructured C and N cytoplasmic termini, a cytoplasmic loop and two extra-cellular loops1.

Connexins oligomerize among the family members to form different structures and Cx47 was shown to form gap junction channels together with Cx43.

These voltage gated channels exhibit ionic and chemical rectification, which creates a directional diffusion barrier for the movement of ions and negatively charged molecules from cells expressing Cx47 to those with Cx431.

The channel mediates an intercellular communication between oligodendrocytes and between astrocytes. This process is essential to maintain the integrity of myelin and maintaining K+ buffering and nutrient homeostasis in oligodendrocytes, myelin and oligodendrocyte function2,3, and indeed, mutations in human Cx47 cause severe myelin disorders2.

Cx43/Cx47 gap junction channels activity was shown to be associated with the development of Multiple Sclerosis and autoimmune demyelination, underscoring a critical role for Cx47 in regulating neuroinflammation4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 25/07/2021

Anti-Connexin-47 (GJC2) Antibody (#ACC-222) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Connexin-47 from rat, mouse and human samples.

For research purposes only, not for human use
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