- Peptide CSSHFPYSQYQFWKN, corresponding to amino acid residues 178-192 of human CCR5 (Accession P51681). 2nd extracellular loop.
- Human promyelocytic leukemia HL-60, acute monocytic leukemia THP-1 and Chronic myelogenous leukemia MEG-O1 cell lines (1:400-1:2000).
- Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 4), human THP-1 acute monocytic leukemia (lanes 2 and 5) and human MEG-O1 Chronic myelogenous leukemia (lanes 3 and 6) cell lysates:1-3. Anti-Human CCR5 (extracellular) Antibody (#ACR-025), (1:400).
4-6. Anti-Human CCR5 (extracellular) Antibody, preincubated with Human CCR5 (extracellular) Blocking Peptide (#BLP-CR025).
CCR5 protein is a G-protein coupled receptor (GPCR) responsible for regulating host immune responses and functions against pathogens. The protein plays a key role in transferring immune cells to inflammatory sites and injury. CCR5 is also considered to be an anchor protein for R5-tropic strains of HIV-1 (Human Immunodeficiency Virus-1) to infect immune cells. Thus, CCR5 contributes to the development of AIDS (Acquired Immunodeficiency Syndrome).
The receptor is widely expressed in a variety of immune cells involved in the inflammatory responses such as macrophages, monocytes, T cells and dendritic cells1,2.
CCR5 is the receptor for the CC ligand 3 (CCL3), CC ligand 4 (CCL4), and CC ligand 5 (CCL5) chemokine ligands. CCR5 and its ligands are upregulated during HIV infection.
Studies have shown that lack of homozygosity of CCR5Δ32 variant, a 32 base-pair deletion of the CCR5’s gene coding region, is associated with preventing the risk of HIV-1 infection2,3.