Anti-KCNK3 (TASK-1) Antibody

Potassium channel subfamily K member 3, TWIK-related acid sensitive K+ channel, K2P3.1, cTBAK-1, OAT1
Cat #: APC-024
Alternative Name Potassium channel subfamily K member 3, TWIK-related acid sensitive K+ channel, K2P3.1, cTBAK-1, OAT1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, r
    May also work in: m
      • Peptide (C)EDEKRDAEHRALLTRNGQ, corresponding to amino acid residues 252-269 of human KCNK3 (Accession O14649). Intracellular, C-terminal part.
    Accession (Uniprot) Number O14649
    Gene ID 3777
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 17/18 amino acid residues identical. Homology with human TASK-3: 11/17 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, ip, wb
    May also work in: ifc
      • Western blot analysis of rat brain (1, 3) and pancreas (2, 4) membranes:
        1,2.  Anti-KCNK3 (TASK-1) Antibody (#APC-024), (1:200).
        3,4.  Anti-KCNK3 (TASK-1) Antibody, preincubated with the control peptide.
      • Transfected HeLa cells (1:1000) (Renigunta, V. et al. (2014) Mol. Biol. Cell 25, 1877.).
      • Rat brain sections.
      • Human bronchial epithelial cells (Telles, C.J. et al. (2016) Am. J. Physiol. 311, C884.).
      • KCNK3 (also named TWIK-related acid sensitive K+ channel, TASK-1 or K2P3.1) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time- or voltage-dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.

        The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. The functional channel is believed to be a dimer.

        K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators.

        KCNK3 is extremely sensitive to variations in external pH, a drop in pH from 7.7 to 6.7 will be enough to close most KCNK3 channels.

        KCNK3 channel distribution is relatively wide with high expression in the brain and heart as well as in the pancreas, kidney and lung.

        KCNK3 can exist in the brain either as a homodimer or as a heterodimer with the closely related channel K2P9.1 (TASK-3).

        During periods of high neuronal activity a transient increase in extracellular pH has been recorded. This pH increase may stimulate KCNK3 channel activity and hence enhance outward K+ currents. This in turn will decrease excitability in these neurons and work as a feedback mechanism.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-KCNK3 (TASK-1) Antibody
    Expression of TASK-1 in rat myocytes.Immunocytochemical staining of rat ventricular myocytes with Anti-KCNK3 (TASK-1) Antibody (#APC-024). A. TASK-1 colocalizes with wheat germ agglutinin (WGA). TASK-1 staining is eradicated when antibody is incubated with the peptide antigen (“-“ panel). B. High resolution of panels in A.Adapted from Jones, S.A. et al. (2002) Am. J. Physiol. 283, 181. with permission of The American Physiological Society.
    Last update: 10/06/2019

    Anti-KCNK3 (TASK-1) Antibody (#APC-024) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation applications. It has been designed to recognize TASK-1 channel from mouse, rat, and human samples.

    For research purposes only, not for human use
    Citations
      • Rat brain lysate (1:100).
        Puissant, M.M. et al. (2017) Front. Cell. Neurosci. 11, 34.
      • Human atrium lysate (1:200).
        Schmidt, C. et al. (2015) Circulation 132, 82.
      • Transfected HeLa cells (1:1000).
        Renigunta, V. et al. (2014) Mol. Biol. Cell 25, 1877.
      • Human bronchial epithelial cells.
        Telles, C.J. et al. (2016) Am. J. Physiol. 311, C884.
      • Rat ventricular myocytes.
        Jones, S.A. et al. (2002) Am. J. Physiol. 283, 181.
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