Anti-KCNK9 (TASK-3) (extracellular) Antibody

Potassium channel subfamily K member 9, K2P9.1, TWIK-related acid-sensitive K+ channel 3, Two pore K+ channel KT3.2
    Cat #: APC-044
    Alternative Name Potassium channel subfamily K member 9, K2P9.1, TWIK-related acid-sensitive K+ channel 3, Two pore K+ channel KT3.2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, r
      • Peptide (C)DDYQQLELVILQSEPHR, corresponding to amino acid residues 57-73 of rat KCNK9 (accession number Q9ES08). Extracellular, near the P1 loop.
        Anti-KCNK9 (TASK-3) (extracellular) Antibody
    Accession (Uniprot) Number Q9ES08
    Gene ID 84429
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human - 15/17 amino acid residues identical.
    RRID AB_2039953.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.75 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*, lci*
      • Anti-KCNK9 (TASK-3) (extracellular) Antibody
        Western blot analysis of KCNK9 in rat cerebellum (lanes 1, 4), human mammary gland MCF-10A (lanes 2, 5) and MCF-7 (lanes 3, 6) cell lysates (upper panel): 
        1-3. Anti-KCNK9 (TASK-3) (extracellular) Antibody (#APC-044).
        4-6. Anti-KCNK9 (TASK-3) (extracellular) Antibody, preincubated with the control peptide antigen. 
        Lower panel: anti-β actin to ensure equal loading.  
        Note that the expected 50 kD MW band is present in cerebellum and in the tumorigenic breast cancer cell line (MCF-7) but not in the non-tumorigenic breast cell line (MCF-10A).
        Anti-KCNK9 (TASK-3) (extracellular) Antibody
        Western blot analysis of rat cerebellum lysate:
        1. Anti-KCNK9 (TASK-3) (extracellular) Antibody (#APC-044), (1:200).
        2. Anti-KCNK9 (TASK-3) (extracellular) Antibody, preincubated with the control peptide antigen.
      • Rat brain sections (Okamoto, K. et al. (2016) eNeuro 3, e0138-16.2016.).
    References
      • KCNK9 (also named TASK3 or K2P9.1) is a member of the 2-pore (2P) domain K+ channels family that probably assemble as dimers to constitute a functional channel.1 These channels show little time- or voltage-dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.  

        The channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc.2 The physiological role of the K2P channels is not yet clear, though they were proposed to participate in breathing, aldosterone secretion and anesthetic-mediated neuronal activity.

        In human, expression of KCNK9 is restricted in brain and its expression overlaps with the structurally related KCNK3 (TASK1, K2P3.1) channel. In rat, it is more widely expressed in other tissues as well as in brain.  

        Unexpectedly, KCNK9 was found to be over-expressed between 5 to 100-fold in 44% of breast cancer tumors tested.  
        Transfection and over-expression of KCNK9 in cell lines promotes tumor growth and confers resistance to hypoxia and serum deprivation.3 Recently, KCNK9 was found to play an important role in K+-dependent apoptosis of cerebellar granules.4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 08/11/2019

    Anti-KCNK9 (TASK-3) (extracellular) Antibody (#APC-044) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize TASK-3 from rat and human samples.

    For research purposes only, not for human use