Anti-KV1.8 Antibody

Voltage-gated K+ channel subunit KV1.8, Cyclic GMP Gated Potassium Channel, KCNA10
    Cat #: APC-157
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)KDPETLLPTNDIHR, corresponding to amino acid residues 187- 200 of human KV1.8 (Accession Q16322). Intracellular, N-terminus.
    Accession (Uniprot) Number Q16322
    Gene ID 3744
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse  - 13/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic, ifc, ih, ip
    Western blot
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart lysates:
    1-2. Anti-KV1.8 Antibody (#APC-157), (1:800).
    3-4. Anti-KV1.8 Antibody, preincubated with the control peptide antigen.
    References
    1. Yao, X. et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 11711.
    2. Tian, S. et al. (2002) Am. J. Physiol. 283, F142.
    3. Yao, X. et al. (2002) J. Am. Soc. Nephrol. 13, 2831.
    4. Orias, M. et al. (1997) Genomics 42, 33.
    5. Lee, S.I. et al. (2013) Hear. Res. 300, 1.
    6. Iwasa, H. et al. (2001) J. Hum. Genet. 46, 549.
    Scientific background

    Potassium (K+) channels regulate cell membrane potential and modulate a number of important cellular functions. K+ voltage-gated channel subfamily A member 10 (KCNA10) also known as KV1.8 is a voltage-gated K+ (KV) channel gene related to the Shaker family of K+ channels that includes eight members (KV1.1- KV1.8)1. KV1.8 contains six membrane-spanning domains with a shaker-type repeat in the fourth segment and a pore (P) region. Its most distinguishing feature is the presence of a putative cyclic nucleotide-binding (CNB) domain at the COOH terminus2. It is specifically regulated by cGMP and postulated to mediate the effects of substances that increase intracellular cGMP1. The channel displays an unusual inhibitor profile, because in addition to being blocked by classical K+ channel blockers, it is also sensitive to inhibitors of cyclic nucleotide gated cation channel such as verapamil and pimozide3.

    KV1.8 is detected in kidney, heart, and aorta by Northern blot and postulated to participate in renal K+ metabolism and to regulate vascular tone3. A recent study showed that a null mutation of mouse KCNA10 causes significant vestibular and mild hearing dysfunction4 In addition KCNA10 has been associated with Long QT syndrome (LQTS), an arrhythmogenic disorder characterized by prolongation of the QT interval on electrocardiograms (ECGs)5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/10/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KV1.8. Anti-KV1.8 Antibody (#APC-157) can be used in western blot analysis. It has been designed to recognize KV1.8 from rat, mouse and human samples.

    For research purposes only, not for human use