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Anti-KV1.8 Antibody

Potassium voltage-gated channel subfamily A member 10, Cyclic GMP gated potassium channel, KCNA10, KCN1
Cat #: APC-157
Alternative Name Potassium voltage-gated channel subfamily A member 10, Cyclic GMP gated potassium channel, KCNA10, KCN1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KDPETLLPTNDIHR, corresponding to amino acid residues 187-200 of human KV1.8 (Accession Q16322). Intracellular, N-terminus.
Accession (Uniprot) Number Q16322
Gene ID 3744
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse  - 13/14 amino acid residues identical.
RRID AB_2341039.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart lysates:
    1,2. Anti-KV1.8 Antibody (#APC-157), (1:800).
    3,4. Anti-KV1.8 Antibody, preincubated with Kv1.8 Blocking Peptide (#BLP-PC157).
References
  1. Yao, X. et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 11711.
  2. Tian, S. et al. (2002) Am. J. Physiol. 283, F142.
  3. Yao, X. et al. (2002) J. Am. Soc. Nephrol. 13, 2831.
  4. Orias, M. et al. (1997) Genomics 42, 33.
  5. Lee, S.I. et al. (2013) Hear. Res. 300, 1.
  6. Iwasa, H. et al. (2001) J. Hum. Genet. 46, 549.
Scientific background

Potassium (K+) channels regulate cell membrane potential and modulate a number of important cellular functions. K+ voltage-gated channel subfamily A member 10 (KCNA10) also known as KV1.8 is a voltage-gated K+ (KV) channel gene related to the Shaker family of K+ channels that includes eight members (KV1.1- KV1.8)1. KV1.8 contains six membrane-spanning domains with a shaker-type repeat in the fourth segment and a pore (P) region. Its most distinguishing feature is the presence of a putative cyclic nucleotide-binding (CNB) domain at the COOH terminus2. It is specifically regulated by cGMP and postulated to mediate the effects of substances that increase intracellular cGMP1. The channel displays an unusual inhibitor profile, because in addition to being blocked by classical K+ channel blockers, it is also sensitive to inhibitors of cyclic nucleotide gated cation channel such as verapamil and pimozide3.

KV1.8 is detected in kidney, heart, and aorta by northern blot and postulated to participate in renal K+ metabolism and to regulate vascular tone3. A recent study showed that a null mutation of mouse KCNA10 causes significant vestibular and mild hearing dysfunction4. In addition KCNA10 has been associated with Long QT syndrome (LQTS), an arrhythmogenic disorder characterized by prolongation of the QT interval on electrocardiograms (ECGs)5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KV1.8. Anti-KV1.8 Antibody (#APC-157) can be used in western blot analysis. It has been designed to recognize KV1.8 from rat, mouse and human samples.

For research purposes only, not for human use
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