Anti-KV1.7 Antibody

Voltage-gated K+ channel KV1.7, KCNA7
Cat #: APC-063
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m
    May also work in: r
    Immunogen
    Peptide TTRKAQEIHGKAPG(C), corresponding to amino acid residues 2-15 of mouse KV1.7 (Accession Q17ST2 ).  Intracellular, N-terminus.
    Accession (Uniprot) Number Q17ST2
    Gene ID 16495
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity The antibody does not recognize human Kv1.7.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic, ifc, ih, ip
    Western blot
    Western blot analysis of mouse heart membranes:
    1. Anti-KV1.7 Antibody (#APC-063), (1:200).
    2. Anti-KV1.7 Antibody, preincubated with the control peptide antigen.
    References
    1. Kalman, K. et al. (1998) J. Biol. Chem. 273, 5851.
    2. Kashuba, V.I. et al. (2001) Gene 268, 115.
    3. Bardien-Kruger, S. et al. (2002) Eur. J. Hum. Genet.10, 36.
    Scientific background

    The KV1.7 voltage-gated K+ channel, is a member of the Shaker family of K+ channels that today includes eight members (KV1.1- KV1.8). KV1.7 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains and intracellular N and C termini. As with other channels of the Shaker subfamily, KV1.7 can readily form heteromers with other members of the subfamily to compose the tetramer that forms the functional channel.

    KV1.7 mRNA was found to be expressed mainly in the heart with somewhat lower levels in pancreas and skeletal muscle.

    The biophysical and pharmacological properties of the KV1.7 channel closely resembles that of the ultra-rapidly activating delayed rectifier (IKur) in cardiac tissue. This current plays a central role in cardiac atrial repolarization that was largely believed to correspond to the activity of the KV1.5 channel. This raises the possibility that the IKur current is the result of a heteromeric KV1.5/KV1.7 channel.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 06/11/2018

    Anti-KV1.7 Antibody (#APC-063) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize KV1.7 from mouse and rat samples. The antibody will not recognize the protein from human samples.

    For research purposes only, not for human use