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Anti-Mouse KV1.7 (KCNA7) Antibody

Potassium voltage-gated channel subfamily A member 7
Cat #: APC-063
Alternative Name Potassium voltage-gated channel subfamily A member 7
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m
Immunogen
  • Peptide TTRKAQEIHGKAPG(C), corresponding to amino acid residues 2-15 of mouse KV1.7 (Accession Q17ST2). Intracellular, N-terminus.
Accession (Uniprot) Number Q17ST2
Gene ID 16495
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse only.
RRID AB_2040160.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity The antibody does not recognize KV1.7 from rat and human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of mouse heart membranes:
    Western blot analysis of mouse heart membranes:
    1. Anti-Mouse KV1.7 (KCNA7) Antibody (#APC-063), (1:200).
    2. Anti-Mouse KV1.7 (KCNA7) Antibody, preincubated with Mouse Kv1.7/KCNA7 Blocking Peptide (#BLP-PC063).
References
  1. Kalman, K. et al. (1998) J. Biol. Chem. 273, 5851.
  2. Kashuba, V.I. et al. (2001) Gene 268, 115.
  3. Bardien-Kruger, S. et al. (2002) Eur. J. Hum. Genet. 10, 36.
Scientific background

The KV1.7 voltage-gated K+ channel, is a member of the Shaker family of K+ channels that today includes eight members (KV1.1-KV1.8). KV1.7 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains and intracellular N- and C-termini. As with other channels of the Shaker subfamily, KV1.7 can readily form heteromers with other members of the subfamily to compose the tetramer that forms the functional channel.

KV1.7 mRNA was found to be expressed mainly in the heart with somewhat lower levels in pancreas and skeletal muscle.

The biophysical and pharmacological properties of the KV1.7 channel closely resembles that of the ultra-rapidly activating delayed rectifier (IKur) in cardiac tissue. This current plays a central role in cardiac atrial repolarization that was largely believed to correspond to the activity of the KV1.5 channel. This raises the possibility that the IKur current is the result of a heteromeric KV1.5/KV1.7 channel.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Anti-Mouse KV1.7 (KCNA7) Antibody (#APC-063) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize KV1.7 from mouse samples. The antibody will not recognize the protein from human or rat samples.

For research purposes only, not for human use
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