Anti-KV1.5 (KCNA5) Antibody

Potassium voltage-gated channel subfamily A member 5
Cat #: APC-004
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    GST fusion protein with sequence HRETDHEEQAALKEEQGIQRRESGLDTGGQRKVSCSKASFHKTGGPLEST DSIRRGSCPLEKCHLKAKSNVDLRRSLYALCLDTS RETDL, corresponding to amino acid residues 513-602 of mouse KV1.5 (Accession Q61762), (MW: 37 kDa.). Intracellular, C-terminus.
    Accession (Uniprot) Number Q61762
    Gene ID 16493
    Peptide confirmation Confirmed by DNA sequence and SDS-PAGE.
    Homology Rat - 86/90 amino acid residues identical; rabbit - 71/90 amino acid residues identical; human - 70/90 amino acid residues identical; bovine, dog - 66/90 amino acid residues identical.
    Purity The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and from antibodies cross-reactive to other KV1 by affinity chromatography on immobilized KV1.3-GST-fusion proteins, and then the antibody was affinity purified on immobilized KV1.5-GST.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.6 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 μl PBS.
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 3 µg fusion protein per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, ip, wb
    May also work in: ifc
    Western blot
    Western blot analysis of rat brain membranes:
    1. Anti-KV1.5 (KCNA5) Antibody (#APC-004), (1:100).
    2. Anti-KV1.5 (KCNA5) Antibody, preincubated with the control peptide antigen.
    Human myeloid dendritic cells (1:500) (Zsiros, E. et al. (2009) J. Immunol. 183, 4483.).
    Immunoprecipitation
    For examples see Product Citations.
    Immunohistochemistry
    Rat brain sections (see also publications using this product).

    Mouse heart sections (1 µg/ml) (Roepke, T.K. et al. (2008) FASEB J 22, 3648.).
    Immunocytochemistry
    Rat cortical astrocytes (1:200) (MacFarlane, S.N. and Sontheimer, H. (2000) J. Neurosci. 20, 5245.).
    References
    1. Swanson, R. et al. (1990) Neuron 4, 929.
    2. Gutman, G. A. et al. (2005) Pharmacol. Rev. 57, 473.
    3. McGahon, M.K. et al. (2007) Am. J. Physiol. Heart Circ. Physiol. 292, H1001.
    Scientific background

    KV1.5 is a mammalian voltage dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.5 was first cloned from the rat brain.1 Eight Shaker related genes exist in mammals constituting the KV1, subfamily of the large KV channel family of genes.2

    A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.

    The structure of KV1.5 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2

    The channel is expressed in cardiac and smooth muscle tissue (colon, aorta, stomach and pulmonary artery) as well as in neurons and kidney.2 A loss-of-function mutation in the gene encoding the channel was found in atrial fibrillation patients, stressing its role as a cardiac action potential regulator.3

    The functional channel is considered transient (A-type) channel and shows prominent inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.

    KV1.5 channels are sensitive to high doses of TEA (330 mM) and low doses of 4-AP (0.27 mM), the “classical” non-selective potassium channel blockers.2

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 20/02/2019

    Anti-KV1.5 (KCNA5) Antibody (#APC-004) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation applications. It has been designed to recognize KV1.5 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
    Western blot citations
    1. Mouse heart lysate.
      Tur, J. et al. (2016) Am. J. Physiol. 312, H571.
    2. Mouse cardiac HL-1 cell lysate.
      Lu, Y.Y. et al. (2016) J. Cell. Mol. Med. 20, 1182.
    3. HEK 293 transfected cells.
      Li, P. et al. (2015) J. Mol. Cell. Cardiol. 86, 138.
    4. Mouse sarcolemmal protein lysate.
      Ednie, A.R. and Bennett, E.S. (2015) J. Biol. Chem. 290, 2769.
    5. Human lymphomas (1:500).
      Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779.
    6. Mouse activated macrophages (BMDM cells).
      Moreno, C. et al. (2013) J. Immunol. 191, 6136.
    7. Rat lung lysate.
      Lv, Y. et al. (2013) Am. J. Physiol. 305, L856.
    8. Mouse heart lysate.
      Huang, H. et al. (2013) J. Mol. Cell. Cardiol. 59, 151.
    9. Human myeloid dendritic cells (1:500).
      Zsiros, E. et al. (2009) J. Immunol. 183, 4483.
    Immunoprecipitation citations
    1. HEK 293 transfected cells.
      Li, P. et al. (2015) J. Mol. Cell. Cardiol. 86, 138.
    Immunohistochemistry citations
    1. Human lymphomas (1:100).
      Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779.
    2. Mouse heart sections (1 µg/ml).
      Roepke, T.K. et al. (2008) FASEB J 22, 3648.
    Immunocytochemistry citations
    1. HEK 293 transfected cells.
      Jimenez-Perez, L. et al. (2016) J. Biol. Chem. 291, 3569.
    2. HEK 293 transfected cells.
      Li, P. et al. (2015) J. Mol. Cell. Cardiol. 86, 138.
    3. Mouse leukaemia monocyte macrophage cells (raw 264.7), human Jurkat T-cells, human Raji B-lymphocytes .
      Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779.
    4. Rat cortical astrocytes (1:200).
      MacFarlane, S.N. and Sontheimer, H. (2000) J. Neurosci. 20, 5245.
    More product citations
    1. Christophersen, I.E. et al. (2013) Eur. Heart J. 34, 1517.
    2. Chen, W.L. et al. (2012) PLoS ONE 7, e49758.
    3. Chung, Y.H. et al. (2000) Brain Res. 875, 164.
    4. Maruoka, N.D. et al. (2000) FEBS Letters 473, 188.
    5. Peretz, A. et al. (2000) The EMBO J. 19, 4036.
    6. Yamashita, T. et al. (2000) Circulation 101, 2007.
    7. Clement-Chomienne, O. et al. (1999) J. Physiol. 515.3, 653.
    8. Hu, X.Q. et al. (1998) J. Biol. Chem. 273, 5337.
    9. Sobko, A. et al. (1998) J. Neuroscience 18, 10398.
    10. Sobko, A. et al. (1998) EMBO J. 17, 4723.
    11. Guo, W. et al. (1997) Am. J. Physiol. 272, H2599.
    12. Guo, W. et al. (1997) Eur. J. Physiol. 434, 206.
    Related Products
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        1. Anti-KV1.5 (KCNA5)-ATTO-550 Antibody (#APC-004-AO).
          A fluorescent primary antibody. It can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit.