Anti-KCNQ4 Antibody

KV7.4, Potassium voltage-gated channel subfamily KQT member 4, KQT-like 4, KvLQT4, DFNA2
    Cat #: APC-164
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    May also work in: h
    Immunogen
    Peptide (C)DRKTREKGDKGPSD, corresponding to amino acid residues 594-607 of mouse KCNQ4 (Accession Q9JK97). Intracellular, C-terminus.
    Accession (Uniprot) Number Q9JK97
    Gene ID 60613
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - identical; human - 12/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
    1-3. Anti-KCNQ4 Antibody (#APC-164), (1:200).
    4-5. Anti-KCNQ4 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of KCNQ4 (KV7.4) in rat lateral dorsal raphe nucleus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-KCNQ4 Antibody (#APC-164), (1:400), followed by biotin-labeled anti-rabbit antibody and streptavidin-ATTO-594 (red). Staining appears in soma of neuronal profiles (arrows). Nuclei are stained with DAPI (blue).
    References
    1. Brown, D.A. and Adams, P.R. (1980) Nature 283, 673.
    2. Coucke, P.J. et al. (1999) Hum. Mol. Genet. 8, 1321.
    3. Yu, H. et al. (2011) ACS Chem. Neurosci2, 572.
    4. Kubisch, C. et al. (1999) Cell 96, 437.
    Scientific background

    The KCNQ (KV7) family of channels consists of five members (KCNQ1KCNQ2KCNQ3, KCNQ4 and KCNQ5). KCNQ channels commonly display activation at voltages close to neuronal resting membrane potentials and are regulated by G-protein coupled receptor (GPCR) signaling, notably by muscarinic receptors1.

    The KCNQ4 (KV7.4) encodes a protein of 695 amino acid residues. The homology with other members of the KCNQ family ranges from 37 to 44% at the amino acid level. KCNQ4 protein contains six domains that span the plasma membrane, and a P-loop domain which forms the K+-selective channel pore. The fourth transmembrane domain contains the voltage sensor, which is responsible for the electrically driven conformational change that leads to channel opening. Functional channels are formed by a tetrameric assembly of KCNQ4 subunits, typically in homotetrameric form, but heterotetrameric co-assembly of different members is allowed2.

    KCNQ2-KCNQ5 are predominantly expressed in the peripheral and central nervous system, including hippocampal cells, cortical cells, and dorsal root ganglion3. In addition KCNQ4 is expressed in the heart and in the outer, but not in the inner, sensory hair cells of the cochlea4.

    It has been demonstrated that KCNQ4 mutations may be a relatively frequent cause of autosomal dominant hearing loss4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 07/01/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse KCNQ4 (KV7.4) channel. Anti-KCNQ4 Antibody (#APC-164) can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV7.4 from rat, human, and mouse samples.

    For research purposes only, not for human use