Name: Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody
Brand: Alomone Labs
SKU: APR-012-AR

Cat #: APR-012-AR

Alternative Name P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KTTRPFKTSNPKNLLGAK, corresponding to amino acid residues 125-142 of human P2RY12 (Accession Q9H244). Intracellular, 2^nd cytoplasmic loop.

Accession (Uniprot) Number Q9H244

Gene ID 64805

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse - 16/18 amino acid residues identical.

RRID AB_10918469.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-012), and immunohistochemistry (labeled antibody).

Applications: if, ih

May also work in: ic*

Immunohistochemistry

Rat striatum frozen section (1:25).
Expression of P2RY12 in rat striatum
Immunohistochemical staining of rat striatum using Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody (#APR-012-AR). P2Y12 (red) appears in patches (white arrows) of myelinated axons. DAPI is used as the counterstain (blue).

Scientific background

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1,P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.^1-3

The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca²⁺ upon activation.

The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting.⁴ The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders.⁴

Lyophilized Powder

Last update: 08/01/2023

For research purposes only, not for human use

Specifications

Citations

Applications

Scientific Background

Shipping and Ordering information

Control Product

P2Y12 Receptor Blocking Peptide (#BLP-PR012) is the original antigen used for immunization during Anti-P2Y12 Receptor Antibody (#APR-012) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y12 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

P2Y12 Receptor Blocking Peptide (#BLP-PR012)

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

Free Sample Program

Not sure whether the product fits your experimental setup?

Try it first!

Alomone Guarantee

We offer 100% guarantee on all our products. If our products do not perform as stated, please contact our technical support staff.

Alomone Guarantee information

Order Summary

Certificate of Analysis

Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody

Application key:

Species reactivity key:

Specifications

Citations

Applications

Scientific Background

Resources

Need Help With This Product?