Anti-VGLUT1 Antibody

Vesicular glutamate transporter 1, Brain-specific Na+-dependent inorganic phosphate cotransporter, BNPI, SLC17A7
    Cat #: AGC-035
    Alternative Name Vesicular glutamate transporter 1, Brain-specific Na+-dependent inorganic phosphate cotransporter, BNPI, SLC17A7
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)EFRQEEFRKLAGR, corresponding to amino acid residues 2-14 of rat VGLUT1 (Accession Q62634). Cytoplasmic, N-terminus.
    • Anti-VGLUT1 Antibody
    Accession (Uniprot) Number Q62634
    Gene ID 116638
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human  – identical.
    RRID AB_2756599.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Anti-VGLUT1 Antibody
      Western blot analysis of rat cortex lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
      1,2. Anti-VGLUT1 Antibody (#AGC-035), (1:200).
      3,4. Anti-VGLUT1 Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-VGLUT1 Antibody
      Expression of VGLUT1 in rat cortex
      Immunohistochemical staining of perfusion-fixed rat brain frozen sections using Anti-VGLUT1 Antibody (#AGC-035), (1:400), followed by goat-anti-rabbit-Alexa-488. VGLUT1 staining (green) appears in neurons of the pyramidal layer of the parietal cortex (arrows). DAPI is used as the nuclear counterstain (blue).
    References
    1. Li, T. et al. (2005) World. J. Gastroenterol. 11, 1731.
    2. Foss, S.M. et al. (2013) J. Neurosci. 33, 10647.
    3. King, M.V. et al. (2014) Neuropsychopharmacology. 39, 464.
    Scientific background

    Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system (CNS) and is responsible for most of the fast synaptic neurotransmission. Glutamate has great contribution for learning, memory, and neuronal plasticity. Dysregulation of glutamate transmission plays a role in the pathophysiology of several psychiatric and neurological diseases1

    Three vesicular glutamate transporters (VGLUT1, VGLUT2, and VGLUT3) have been identified. They are responsible for packaging and transporting glutamate into synaptic vesicles. VGLUTs in part regulate the storage and release of glutamate in excitatory circuits in the brain.

    All three VGLUT isoforms are highly conserved and have the same predicted topology including 12 putative transmembrane domains. They all share a conserved dileucine-like motif in the C terminus. This motif was found to be important for VGLUT1 endocytosis. In addition, VGLUT1 contains two polyproline domains distal to the C-terminal dileucine-like motif, including one that interacts with the endocytic BAR (Bin/Amphiphysin/Rvs) domain protein endophilin. Two additional dileucine-like motifs are present in the N terminus of VGLUT1 that are not well conserved in the other isoforms1-3

    VGLUT1 is expressed in the hippocampus, cerebral cortex, and cerebellar cortex and appears to be the main isoform expressed in the cortex. The transporter is also detected in the enteric nervous system and pancreatic tissue. VGLUT1 has been suggested to influence the properties of the release of glutamate1,2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/01/2020

    Anti-VGLUT1 Antibody (#AGC-035) is a highly specific antibody directed against an epitope of rat Vesicular glutamate transporter 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize VGLUT1 from rat, mouse, and human samples.

    For research purposes only, not for human use
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