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L-Ascorbic acid

Vitamin C

A Blocker of T-Type CaV Channels

Cat #: L-140
Alternative Name Vitamin C
Lyophilized Powder yes
  • Bioassay Tested
    • Source Synthetic
    MW: 176.1
    Purity: >98%
    Effective concentration 10 nM - 5 µM.
    Structure
    Chemical name 3-Oxo-L-gulofuranolactone.
    Molecular formula C6H8O6.
    CAS No.: 50-81-7
    Activity IC50 of 6.5 µM on T-type current of rat DRG neurons1. IC50s of 10 and 25 nM on cloned CaV3.2 channels from human and rat, respectively1.
    References-Activity
    1. Nelson, M.T. et al. (2007) J. Neurosci. 27, 12577.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Water. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs L-Ascorbic acid inhibits T-type voltage-gated Ca2+ currents expressed in  Xenopus oocytes.
      Alomone Labs L-Ascorbic acid inhibits T-type voltage-gated Ca2+ currents expressed in  Xenopus oocytes.
      A. Time course of CaV3.2/β3 (T-type) current inhibition by 5 µM of L-Ascorbic acid (#L-140). Currents were elicited by application of voltage ramps from a holding potential of -100 mV to 20 mV (100 msec) delivered every 10 seconds. B. Superimposed example traces of current responses before and during perfusion of 5 µM L-Ascorbic acid as indicated.
    References - Scientific background
    1. Nilius, B. et al. (2006) Cell Calcium 40, 81.
    2. Perez-Reyes, E. and Lory, P. (2006) CNS Neurol. Disord. Drug Targets 5, 605.
    3. Talavera, K. and Nilius, B. (2006) Cell Calcium 40, 97.
    4. Nelson, M.T. et al. (2007) J. Neurosci. 27, 12577.
    Scientific background

    The T-type calcium channel belongs to the voltage-gated Ca2+ channel (CaV) family. "T" stands for transient, referring to the length of activation. As with other sub-types of voltage-gated Ca2+ channels, the α1 subunit is the one that determines most of the channel's properties. T-type calcium channels may contain one of three α1 subunits, α1G (CaV3.1), α1H (CaV3.2) or α1I (CaV3.3)1-3.

    T-type Ca2+ channels are involved in the control of neuronal excitability and their gating can be modulated by a variety of redox agents4.

    L-Ascorbic acid (ascorbate) is an endogenous redox agent that selectively inhibits native CaV3.2 channels in the peripheral and central neurons4.

    L-Ascorbic acid has an IC50 of 6.5 µM on T-type current of rat DRG neurons, and IC50s of 10 and 25 nM on cloned CaV3.2 channels from human and rat, respectively4.

    Target T-type Ca2+ channels
    Lyophilized Powder
    Last update: 02/01/2022

    L-Ascorbic acid (#L-140) is a highly pure, synthetic, and biologically active compound.

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    For research purposes only, not for human use
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