Certificate of Analysis

Renal Purinergic Receptor Antibody Explorer Kit

A Screening Package of Renal Purinergic Receptor Antibodies Economically Priced
Cat #: AK-645 16 Vials

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-P2X7 Receptor Antibody Cat #: APR-004

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 receptor (Accession Q64663). Intracellular, C-terminus.
Homology
Mouse - 18/20 amino acid residues identical; human - 16/20 amino acid residues identical.

Anti-P2Y1 Receptor Antibody Cat #: APR-009

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)RALI YKDLD NSPLR RKS, corresponding to residues 242-258 of rat or human P2RY1. (Accession P49651P47900). 3rd intracellular loop (i3) between TM5 and TM6 domains.
Homology
Rat, human, bovine - identical; turkey, chicken - 16/17 amino acid residues identical; mouse - 15/17 amino acid residues identical.

Anti-P2Y2 Receptor Antibody Cat #: APR-010

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)KPAY GTTGL PRAKR KSVR, corresponding to residues 227-244 of rat P2RY2 (Accession P41232). 3rd intracellular loop (i3) between TM5 and TM6 domains.
Homology
Mouse - 18/19 amino acid residues identical; human - 17/19 amino acid residues identical.

Anti-P2Y4 Receptor Antibody Cat #: APR-006

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)HEES ISRWA DTHQD, corresponding to residues 337-350 of rat P2RY4 (Accession O35811). Intracellular, C-terminus.
Homology
Mouse - 13/14 amino acid residues identical.

Anti-P2Y6 Receptor Antibody Cat #: APR-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide CQPHDLLQKLTAKWQRQRV, corresponding to amino acid residues 311-328 of rat P2Y6 receptor (Accession Q63371). Intracellular, C-terminus.
Homology
Mouse - 17/18 amino acid residues identical; human - 15/18 amino acid residues identical.

Anti-Adenosine A1 Receptor Antibody Cat #: AAR-006

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)KKVSASSGDPQKYYGKE, corresponding to amino acid residues 213-229 of human A1AR (Accession P30542). 3rd intracellular loop.
Homology
Mouse, rat, bovine, canis and pig - identical.

Anti-Adenosine A2A Receptor Antibody Cat #: AAR-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)RQLKQMESQPLPGER, corresponding to amino acid residues 201-215 of mouse adenosine A2A receptor (Accession Q60613). 3rd intracellular loop.
Homology
Rat, human, and canis – identical.

Anti-Adenosine A2B Receptor (extracellular) Antibody Cat #: AAR-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide KDSATNN*STEPWDGTTNESC, corresponding to amino acid residues 147-166 of human A2BAR with replacement of cysteine 154 (C154) with serine (*S) (Accession P29275). 2nd extracellular loop.
Homology
Rat - 14/19 amino acid residues identical; mouse - 13/19 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.