Overview
- Peptide (C)TTKINMDDLQPSENEDKS, corresponding to amino acid residues 848-865 of rat CaV1.2 (Accession P22002). Intracellular loop between domains II and III.
- Rat brain and rat heart membranes (1:200).
- Human cardiac tissue (Crossman, D.J. et al. (2011) PLoS ONE 6, e17901.).
- Western blot analysis of rat brain membranes:1. Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003), (1:200).
2. Anti-CaV1.2 (CACNA1C) Antibody, preincubated with Cav1.2/CACNA1C Blocking Peptide (#BLP-CC003). - Western blot analysis of CaV1.2-transfected Xenopus oocytes (lane 1) and non-transfected oocytes lysates (lane 2):1. Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003), (1:200) in CaV1.2 (CACNA1C) Channel Overexpressed in Xenopus oocytes.
2. Anti-CaV1.2 (CACNA1C) Antibody in non-transfected oocytes.
- CaV1.2 transfected HEK-293 cells and mouse heart lysate (Rougier, J.S. et al. (2011) J. Biol. Chem. 286, 8829.).
- Rat brain sections (see also 3, 5, 7, 10 of recent publications using this product). Mouse atrioles (1:300) (Howitt, L. et al. (2013) J. Physiol. 591, 2157.).
- Rat insulinoma cells (RIN) (Parkash, J. (2011) Life Sci. 88, 257.).
- Mouse chromaffin cells (1:200). (Perez-Alvarez, A. et al. (2011) J. Neurochem. 116, 105.).
- Rat mast cells (RBL-2H3 cells, 1μg/5x105 cells). (Yoshimaru, T. et al. (2009) Mol. Immunol. 46, 1267.).
- The control antigen is not suitable for this application.
All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal, cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors.
While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous and CaV1.3 channels are found in the heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channel blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700).
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Expression of CaV1.2 in rat cardiomyocytes.Immunocytochemical staining of adult rat cardiomyocytes using Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003). CaV1.2 staining (green) is observed in a regularly spaced array, as well as surface sarcolemmal staining. β-arrestin staining is in red.Adapted from Hermosilla, T. et al. (2017) Sci. Rep. 7, 10131. with permission of SPRINGER NATURE.