Anti-CXCR7 (ACKR3) (extracellular)-ATTO-488 Antibody

Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, CXCR-7, Chemokine orphan receptor 1, G-protein coupled receptor RDC1 homolog, CMKOR1, GPR159
    Cat #: ACR-037-AG
    Alternative Name Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, CXCR-7, Chemokine orphan receptor 1, G-protein coupled receptor RDC1 homolog, CMKOR1, GPR159
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)DVHLFDYVEPGNYSD, corresponding to amino acid residues 2-16 of rat CXCR7 (ACKR3) (Accession O89039). Extracellular, N-terminus.
    • Anti-CXCR7 (ACKR3) (extracellular)-ATTO-488 Antibody
    Accession (Uniprot) Number O89039
    Gene ID 84348
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse –14/15 amino acid residues identical; human – 12/15 amino acid residues identical.
    RRID AB_2756575.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACR-037), and flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic*, ih*, lci*
    Direct flow cytometry
    • Anti-CXCR7 (ACKR3) (extracellular)-ATTO-488 Antibody
      Cell surface detection of CXCR7 in human HL-60 (A) and human Raji (B) living intact cells:
      ____ Unstained cells
      ____ Cells + Anti-CXCR7 (ACKR3) (extracellular)-ATTO-488 Antibody (#ACR-037-AG), (5 μg/0.5x106 cells).
    References
    1. Griffith, J.W. et al. (2014) Annu. Rev. Immuno. 32, 659.
    2. Sun, X. et al. (2010), Cancer Metastasis Rev. 29, 709.
    3. Burns, J.M. et al. (2006) J. Exp. Med. 203, 2201.
    4. Jones, S.W. et al. (2006) Osteoarthritis and Cartilage 14, 597.
    5. Miao, Z. et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 104, 15735.
    6. Boldajipour, B. et al. (2008) Cell 132, 463.
    Scientific background

    Chemokines are molecules that control the migration and positioning of the immune system cells. In addition to their function as chemo-attractants they have various other roles in the maturation of immune cells such as T-cell development in the thymus and homeostatic retention of immune cells in the bone marrow1.

    Chemokines bind to chemokine receptors, members of the G-protein coupled receptor superfamily. They are comprised of seven transmembrane receptors with extracellular N-terminus, three extracellular and three intracellular loops and an intracellular C-terminus. One of the intracellular domains of the receptor couples with G proteins and begins a cascade of signal transduction2.

    Until recently the CXCR-7 was an “orphan” receptor with its endogenous ligand unknown. This receptor is encoded by the CXCR7/RDC-1 gene which was mapped to mouse chromosome 1 and human chromosome 2. Homology has been shown with the viral gene ORF74 suggesting the receptor might signal without the binding of a ligand. Currently it is known that the chemokines CXCL12 and CXCL11 bind to CXCR-73.

    CXCR-7 is tightly linked to the regulation of B cell development and differentiation indicating it might have a role in the formation of B memory cells4. CXCR-7 was also found to be associated with tumors where its levels are elevated in many tumor cell lines such as endothelial cells, fetal liver cells and placenta cells5. Despite this suggested role, there is growing evidence that this receptor does not function as a regular GPCR6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-CXCR7 (ACKR3) (extracellular) Antibody (#ACR-037) is a highly specific antibody directed against an epitope of the rat CXCR7 chemokine receptor. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize CXCR7 from human, rat and mouse samples.

    Anti-CXCR7 (ACKR3) (extracellular)-ATTO-488 Antibody (#ACR-037-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-CXCR7 (ACKR3) (extracellular)-ATTO-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use
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