- Peptide (C)RLRYNDTFETLILH, corresponding to amino acid residues 131-144 of rat GABRE (Accession Q9ES14). Extracellular, N-terminus.
- Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), human SHSY-5Y neuroblastoma cells lysate (lanes 3 and 7) and human U87 MG glioblastoma cells lysate (lanes 4 and 8):1-4. Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody (#AGA-015), (1:400).
5-8. Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody, preincubated with GABA(A) ε Receptor/GABRE (extracellular) Blocking Peptide (#BLP-GA015).
- Expression of GABA(A) ε receptor in rat and mouse cerebellaImmunohistochemical staining of mouse and rat cerebellum using Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody (#AGA-015). In both mouse (A) and rat (B) cerebellum GABA(A) ε receptor staining (purple) appears in the Purkinje cell body (P and vertical arrows) and in their dendritic tree (horizontal arrow) extending into the molecular layer (MOL). Nucler staining using DAPI as the counterstaining (blue).
- Expression of GABA(A) ε receptor in human U-87 MG cellsCell surface detection of GABA(A) ε receptor in intact living human U-87 MG cells. A. Extracellular staining of cells using Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody (#AGA-015), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody, merged with nuclear staining using DAPI (blue). B. Extracellular staining (red) merged with live view of the cells.
- Owens, D.F. and Kriegstein, A.R. (2002) Nat. Rev. Neurosci. 3, 715.
- Whiting, P.J. (1999) Neurochem. Int. 34, 387.
- Mihic, S.J. and Harris, R.A. (1997) Alcohol Health Res. World 21, 127.
- Neelands, T.R. et al. (1999) J. Neurosci. 19, 7057.
- Olsen, R.W. and Tobbin, A.J. (1990) FASEBS J. 4, 1469.
GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system1.
The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily2. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells3. Binding of GABA to the GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity2.
GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ1. Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consist of 2α, 2β, and 1γ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands.
Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes4. For example, the α4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain5.
Species reactivity key:
Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody (#AGA-015) is a highly specific antibody directed against an extracellular epitope of the rat GABA(A) receptor ε subunit. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and live cell imaging applications. It has been designed to recognize GABA(A) ε receptor from human, rat and mouse samples.