Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody

GABA(A) receptor subunit epsilon, Gamma-aminobutyric acid receptor subunit epsilon
Cat #: AGA-015
Alternative Name GABA(A) receptor subunit epsilon, Gamma-aminobutyric acid receptor subunit epsilon
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RLRYNDTFETLILH, corresponding to amino acid residues 131-144 of rat GABRE (Accession Q9ES14). Extracellular, N-terminus.
    Accession (Uniprot) Number Q9ES14
    Gene ID 65191
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical; human - 10/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc, ip
      • Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), SHSY-5Y lysate (lanes 3 and 7) and U87 MG lysate (lanes 4 and 8):
        1-4. Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody (#AGA-015),  (1:400).
        5-8. Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody, preincubated with the control peptide antigen.
      • Expression of GABA(A) ε receptor in rat and mouse cerebella
        Immunohistochemical staining of mouse and rat cerebellum using Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody (#AGA-015).  In both mouse (A) and rat (B) cerebellum GABA(A) ε receptor staining (purple) appears in the Purkinje cell body (P and vertical arrows) and in their dendritic tree (horizontal arrow) extending into the molecular layer (MOL). Nucler staining using DAPI as the counterstaining (blue).
      • Expression of GABA(A) ε receptor in human U-87 MG cells
        Cell surface detection of GABA(A) ε receptor in intact living human U-87 MG cells. A. Extracellular staining of cells using Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody (#AGA-015), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody, merged with nuclear staining using DAPI (blue). B. Extracellular staining (red) merged with live view of the cells.
      • GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system1.

        The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily2. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells3. Binding of GABA to the GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity2.

        GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ1. Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consist of 2α, 2β, and 1γ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands.

        Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes4. For example, the α4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 04/06/2019

    Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody (#AGA-015) is a highly specific antibody directed against an extracellular epitope of the rat GABA(A) receptor ε subunit. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and live cell imaging applications. It has been designed to recognize GABA(A) ε receptor from human, rat and mouse samples.

    For research purposes only, not for human use