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Anti-NaV1.5 (SCN5A) (1978-2016) Antibody

Sodium channel protein type 5 subunit alpha, SKM2
Cat #: ASC-013
Alternative Name Sodium channel protein type 5 subunit alpha, SKM2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
May also work in: m*
Immunogen
  • GST fusion protein with amino acid residues 1978-2016 of human NaV1.5 (Accession Q14524), (MW: 33 kDa.). Intracellular, C-terminus.
Accession (Uniprot) Number Q14524
Gene ID 6331
Peptide confirmation Confirmed by DNA sequence and SDS-PAGE.
Homology Rat - 36/39 amino acid residues identical; mouse - 34/39 amino acid residues identical.
RRID AB_2039999.
Purity The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.95 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, ifc, ih, wb
May also work in: ip*
Western blot
  • Western blot analysis of NaV1.5 in NaV1.5 transfected HEK-293 cells:
    Western blot analysis of NaV1.5 in NaV1.5 transfected HEK-293 cells:
    1. Anti-NaV1.5 (SCN5A) (1978-2016) Antibody (#ASC-013), (1:200).
    2. Anti-NaV1.5 (SCN5A) (1978-2016) Antibody, preincubated with Nav1.5/SCN5A (1978-2016) Blocking Peptide (#BLP-SC013).
Immunohistochemistry
  • Expression of NaV1.5 in rat cardiac muscle
    Expression of NaV1.5 in rat cardiac muscle
    Immunohistochemical staining of NaV1.5 in rat myocardium paraffin-embedded section using Anti-NaV1.5 (SCN5A) (1978-2016) Antibody (#ASC-013), (1:100). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxilin is used as the counterstain.
Immunocytochemistry
  • Human atrial myofibroblasts (1:200) (Chatelier, A. et al. (2012) J. Physiol. 590, 4307.).
Indirect flow cytometry
  • THP-1 human premyelomonocytic leukemic cells (Carrithers, M.D. et al. (2007) J. Immunol. 178, 7822.).
  • The control antigen is not suitable for this application.
References
  1. Wu, L. et al. (2002) NeuroReport 13, 2547.
  2. Liu, C.J. et al. (2003) J. Biol. Chem. 278, 1029.
Scientific background

Voltage-gated Na+ channels (NaV) are responsible for myocardial conduction and maintenance of the cardiac rhythm and are essential for the generation of action potentials and cell excitability.1

Dysfunction or disregulation of cardiac sodium channels can cause several disorders, including cardiac arrhythmias.  

The majority of Na+ channels in the mammalian heart are Tetrodotoxin (TTX) insensitive NaV1.5.2 

The putative structure of NaV1.5 consists of four homologous domain (I-IV), each containing six transmembrane segments (S1-S6). Mutations in the C-terminus of NaV1.5 were described in connection to Long QT syndrome and Brugada syndrome.1-2 Recent data have demonstrated selective expression of NaV1.5 in the mouse central nervous system and implicated a role for NaV1.5 in the physiology of the central nervous system.1

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title: Anti-Nav1.5 (SCN5A) (1978-2016) Antibody
Expression of NaV1.5 in rat heart.
Immunohistochemical staining of rat heart sections using Anti-NaV1.5 (SCN5A) (1978-2016) Antibody (#ASC-013). NaV1.5 staining (green), (left panel). αB-Crystallin staining (red), (middle panel). Merged image (right panel) demonstrates that the two proteins co-localize in rat heart.Adapted from Huang, Y. et al. (2016) J. Biol. Chem. 291, 11030. With permission of The American Society for Biochemistry and Molecular Biology.
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human NaV1.5 channel. Anti-NaV1.5 (SCN5A) (1978-2016) Antibody (#ASC-013) can be used in western blot, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.5 sodium channel from rat, human, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Published figures using this product
  • Multiplex staining of neuronal NaV channels and Ryanodine receptor 2 in mouse cardiomyocytes.
    Multiplex staining of neuronal NaV channels and Ryanodine receptor 2 in mouse cardiomyocytes.
    Immunocytochemical staining of mouse cardiomyocytes using Anti-SCN1A (NaV1.1) Antibody (#ASC-001), Anti-NaV1.5 (SCN5A) (1978-2016) Antibody (#ASC-013) and Anti-NaV1.6 (SCN8A) Antibody (#ASC-009) antibodies. All three neuronal NaV channels (green) co-localize with Ryanodine receptor 2.
    Adapted from Radwanski, P.B. et al. (2015) with permission of the European Society of Cardiology.
Western blot citations
  1. HEK 293 transfected cells.
    Beltran-Alvarez, P. et al. (2013) FEBS Lett. 587, 3159.
  2. Human cardiac cells.
    Tarradas, A. et al. (2013) PLoS ONE 8, e53220.
Immunohistochemistry citations
  1. Rat heart sections.
    Huang, Y. et al. (2016) J. Biol. Chem. 291, 11030.
Immunocytochemistry citations
  1. Mouse isolated myocytes.
    Radwanski, P.B. et al. (2015) Cardiovasc. Res. 106, 143.
  2. Human atrial myofibroblasts (1:200).
    Chatelier, A. et al. (2012) J. Physiol. 590, 4307.
  3. THP-1 human premyelomonocytic leukemic cells.
    Carrithers, M.D. et al. (2007) J. Immunol. 178, 7822.
Indirect flow cytometry citations
  1. THP-1 human premyelomonocytic leukemic cells.
    Carrithers, M.D. et al. (2007) J. Immunol. 178, 7822.
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