Anti-NMDAR2D (GRIN2D) (extracellular) Antibody

NMDA receptor 2D, NR2D, GluN2D, Ionotropic glutamate receptor subunit ε4, N-methyl-D-aspartate receptor subunit 2D
    Cat #: AGC-020
    Alternative Name NMDA receptor 2D, NR2D, GluN2D, Ionotropic glutamate receptor subunit ε4, N-methyl-D-aspartate receptor subunit 2D
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CRTQNRTHRGESLHR, corresponding to amino acid residues 345-359 of rat NMDAR2D (Accession Q62645). Extracellular, N-terminus.
    • Anti-NMDAR2D (GRIN2D) (extracellular) Antibody
    Accession (Uniprot) Number Q62645
    Gene ID 24412
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - 14/15 amino acid residues identical.
    RRID AB_10658334.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*, lci*
    Western blot
    • Anti-NMDAR2D (GRIN2D) (extracellular) Antibody
      Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
      1,2. Anti-NMDAR2D (GRIN2D) (extracellular) Antibody (#AGC-020), (1:200).
      3,4. Anti-NMDAR2D (GRIN2D) (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    • Anti-NMDAR2D (GRIN2D) (extracellular) Antibody
      Expression of NMDA receptor 2D in rat reticular thalamic nucleus
      Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NMDAR2D (GRIN2D) (extracellular) Antibody (#AGC-020), (1:200). A. Staining (green) appears in a discreet population of cells (thin arrows). B. The same section was stained for parvalbumin (red) and cell nuclei were visualized with DAPI (blue). Cells positive for NR2D but negative for parvalbumin (thick arrow, shown in A) and cells positive for both NR2D and parvalbumin (vertical arrow) are both present.
    References
    1. Dingledine, R. et al. (1999) Pharmacol. Rev. 51, 7.
    2. Mayer, M.L. and Armstrong, N. (2004) Annu. Rev. Physiol. 66, 161.
    3. Prybylowski, K. and Wenthold, R.J. (2004) J. Biol. Chem. 279, 9673.
    4. Mayer, M.L. (2006) Nature 440, 456.
    Scientific background

    The NMDA receptors (NMDARs) are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.

    The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.

    The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.

    Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson’s and Alzheimer’s disease.

    The expression and function of NMDA receptors are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat NMDA receptor 2D. Anti-NMDAR2D (GRIN2D) (extracellular) Antibody (#AGC-020) can be used in western blot and immunocytochemistry applications. It has been designed to recognize NR2D from rat, mouse, and human samples.

    For research purposes only, not for human use
    Shipping and Ordering information