This product is freeze dried. All water molecules have been removed.
Every lot is tried & tested in a relevant biological assay.
- Garcia-Valdes, J. et al. (1998) FEBS. Lett. 505, 369.
- Alomone Labs Slotoxin inhibits the current of Kca1.1 (mSlo) channels expressed in Xenopus oocytes.A. Representative time course of Kca1.1 current inhibition by 50 nM Slotoxin (#STS-410). Membrane potential was held at -100 mV, current was elicited by a 150 ms voltage ramp to +100 mV every 10 sec, and reversibly inhibited by Slotoxin (as indicated in green). B. Superimposed traces of Kca1.1 current before (black) and after application of 50 nM Slotoxin (green) taken from the recording in A.
- 1. Garcia-Valdes, J. et al. (1998) FEBS. Lett. 505, 369.
Native Slotoxin was originally isolated from Centruroides noxius scorpion venom.
Native Slotoxin blocks the Maxi K+ (BKCa or slo, KCNMA1, KCa1.1) channels.1
Slotoxin blocks differentially channels formed by the α1 subunit alone and channels formed by the α1 combined with auxiliary β subunits. In Xenopus oocytes expressing hSlo (α1 alone), Kd was calculated to be 1.5 nM and the block is complete and reversible. With the additional co-expression of either the β1 or β4 subunits, the block become irreversible or causes the channel to be almost insensitive to the toxin, respectively.1
Slotoxin (#STS-410) is a highly pure, synthetic, and biologically active peptide