Pimaric Acid

PiMA
A Novel Opener of KCa1.1 Channels
Cat #: P-270
Alternative Name PiMA
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Natural product of unspecified plant origin.
    Source Natural
    MW: 302
    Purity: >85%
    Effective concentration 1-10 µM.
    Chemical name 1-Phenanthrenecarboxylicacid,7-ethenyl-1,2,3,4,4a,4b,5,6,7,9,10,10a-dodecahyde.
    Molecular formula C20H30O2.
    CAS No.: 127-27-5.
    Activity Pimaric Acid is a potent opener of large conductance Ca2+ activated K+ (BK) channels. 1 µM of Pimaric Acid doubles the current carried via BK channels. Isopimaric Acid may increase the open probability by shifting the Ca2+ and voltage dependence to more hyperpolarized voltages1. The sensitivity is decreased in the Xenopus system compared to mammalian cells. BK channels activated by Pimaric Acid retain their charybdotoxin sensitivity.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Organic solvents such as DMSO, ethanol, acetone or methanol. DMSO is suggested for biological experiments. DMSO solution should not exceed 0.03%. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to three months at -20°C.
      • Alomone Labs Pimaric Acid was tested in Xenopus oocytes expressing KCa1.1 channel using TEVC recordings.
        Left: Time course of KCa1.1 channel amplitude, by applying 50 µM Pimaric Acid (#P-270) and 200 nM Charybdotoxin (#STC-325), (ChTx). Currents were elicited by a 100 ms pulse to 0 mV from holding potential of -100 mV. Pulses were delivered every 10 seconds. The bars at the top indicate the period of the indicated drug application. Currents were normalized to the values of controls. The red green and blue symbols mark the traces that are shown on the right. Right: Example traces of control (red), Pimaric Acid activated (green) and Charybdotoxin (ChTx) inhibited (blue). KCa1.1 currents are taken from the experiment shown on the left.
        Alomone Labs Pimaric Acid activates KCa1.1 channels heterologously expressed in Xenopus oocytes.
        Pimaric Acid (#P-270) activation of KCa1.1 channels is voltage dependent. The amount of activation by Pimaric Acid was assayed by normalizing each response to its control current amplitude. All responses were simultaneously gathered by using a stepwise voltage protocol.
      • PiMA was found to be a potent opener of KCa1.1 (large conductance Ca2+ activated K+ (BK)) channels. 1 µM PiMA doubled the current carried via BK channels and this effect increased dose-dependently. PiMA increases the open probability of single BK channels by shifting both its voltage- and Ca2+-dependence to more hyperpolarized voltages.1 BK channels that were activated by PiMA retain their charybdotoxin sensitivity.
    Target KCa1.1 K+ channels
    Last update: 10/03/2019

    Pimaric Acid (#P-270) is a highly pure, natural, and biologically active compound.

    For research purposes only, not for human use
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