Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody

AT1 Receptor, AT1R, AGTR1
    Cat #: AAR-011
    Alternative Name AT1 Receptor, AT1R, AGTR1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 receptor (Accession P30556). Extracellular, N-terminus.
    • Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
    Accession (Uniprot) Number P30556
    Gene ID 185
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat, rabbit, dog - identical.
    RRID AB_2040271.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.7 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, lci, wb
    May also work in: ip*
    Western blot
    • Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
      Western blot analysis of mouse kidney (lanes 1 and 3) and mouse heart (lanes 2 and 4) membranes:
      1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:500).
      3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
      Western blot analysis of rat liver (lanes 1 and 3) and rat kidney (lanes 2 and 4) membranes:
      1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:200).
      3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
      Expression of Angiotensin II Receptor Type-1 in mouse cerebellum
      Immunohistochemical staining of mouse cerebellum using Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011). A. Mouse anti-Parvalbumin (red) is detected in the Purkinje layer. B. In the same section, AT1 receptor (green) is also present in the Purkinje layer. Arrows point at AT1 receptor immunoreactive cells. Merge of A and B panels reveals partial co-localization.
    Indirect flow cytometry
    • Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
      Cell surface detection of AGTR1 in live intact human Jurkat T-cell leukemia cells:
      ___ Unstained cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (5 µg) + goat-anti-rabbit-FITC.
      ___ Cells + Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody, (10 µg) + goat-anti-rabbit-FITC.
    • The negative control antigen is not suitable for this application.
    Live cell imaging / Immunocytochemistry
    • Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
      Expression of AGTR1 in rat C6 glioma cells
      Cell surface detection of AGTR1 in live intact rat C6 glioma cells. A. Cells were stained using Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Live intact C6 cells.
    References
    1. Sasaki, K. et al. (1991) Nature 351, 230.
    2. Murphy, T.J. et al. (1991) Nature 351, 233.
    3. De Gasparo, M. et al. (2000) Pharmacol. Rev. 52, 415.
    4. Hunyady, L. and Catt, K.J. (2006) Mol. Endocrinol. 20, 953.
    Scientific background

    Angiotensin II receptor type 1 (AT1 receptor, AGTR1) is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II).
     
    Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects.
     
    Most of the physiological actions of Ang II are mediated by AT1 receptor a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

    The AT1 receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (IP3) and intracellular Ca2+ mobilization. In addition to the rapid actions mediated by IP3 and Ca2+ signaling, AT1 receptor elicits other signaling responses including activation of the MAPK and Jak/STAT. Together, these signaling pathways mediate most of Ang II cellular responses that include, control of hormone secretion, regulation of cell growth and apoptosis, regulation of ion channels activation and cell migration.
     
    In accordance with the pleiotropic actions of Ang II, the AT1 receptor has a wide distribution with high levels detected in the adrenal gland, kidney, brain, heart, liver, etc.
     
    As is the case with many other peptide receptors, the AT1 receptor undergoes ligand-induced endocytosis, a process that contributes to receptor desensitization and sequestration and contributes to regulate receptor signaling.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
    Expression of AT1R in mouse satellite cells and mouse myofibers.A. Indirect flow cytometry of mouse satellite cells using Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011). B. Immunocytochemical staining of intact cultured mouse myofibers using same antibody as in A.Adapted from Yoshida, T. et al. (2013) J. Biol. Chem. 288, 23823. with permission of the American Society for Biochemistry and Molecular Biology.

    Last update: 24/01/2020

    Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize AT1 receptor from rat, mouse, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Rat kidney lysate (1:2000).
      Ismail, B. et al. (2016) EJNMMI Res. 6, 55.
    2. Rat heart lysate (1:1000).
      Lino, C.A. et al. (2015) Mol. Cell. Endocrinol. 416, 1.
    3. Rat brain lysate (1:2000).
      Du, D. et al. (2013) PLoS ONE 8, e70976.
    4. Rat heart lysate (1:250).
      Wang, H. et al. (2013) PLoS ONE 8, 76992.
    5. Mouse lung lysate (1:1000).
      Rodrigues-Machado, M.G. et al. (2013) Br. J. Pharmacol. 170, 835.
    6. Sheep brain medulla lysate (1:200).
      Marshall, A.C. et al. (2013) Peptides 44, 25.
    Immunocytochemistry citations
    1. Mouse isolated myofibers (1:100).
      Yoshida, T. et al. (2013) J. Biol. Chem. 288, 23823.
    Indirect flow cytometry citations
    1. Mouse gastrocnemius muscle cells (1:100).
      Yoshida, T. et al. (2013) J. Biol. Chem. 288, 23823.
    More product citations
    1. Zhang, T. et al. (2011) J. Physiol. 589, 2383.
    2. Day, R.T. et al. (2010) Cell Signal. 22, 1849.
    3. Ferreira, A.J. et al. (2010) Ther. Adv. Cardiovasc. Dis. 4, 83.
    4. Feliers, D. et al. (2010) Mol. Cell. Endocrinol. 314, 136.
    5. Schneider, M.P. et al. (2010) Am. J. Physiol. 299, R899.
    6. Varagic, J. et al. (2010) Am. J. Nephrol. 32, 557.
    7. Downie, L.E. et al. (2009) Neurosience 161, 195.
    8. Sridhar, A. et al. (2009) Cardiovasc. Res. 84, 227.
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