Anti-Aquaporin 6 Antibody

AQP6, AQP2L, Kidney-specific aquaporin
    Cat #: AQP-006
    Alternative Name AQP6, AQP2L, Kidney-specific aquaporin
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
      • Peptide (C)EPQKKESQTNSEDTEVSV corresponding to amino acid residues 259-276 of rat AQP6 (Accession Q9WTY0). Intracellular, C-terminus.
        Anti-Aquaporin 6 Antibody
    Accession (Uniprot) Number Q9WTY0
    Gene ID 29170
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 15/18 amino acid residues identical.
    RRID AB_2039738.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic*, ifc*, ip*
      • Anti-Aquaporin 6 Antibody
        Western blot analysis of rat kidney membranes:
        1. Anti-Aquaporin 6 Antibody (#AQP-006), (1:200).
        2. Anti-Aquaporin 6 Antibody, preincubated with the control peptide antigen.
      • Rat and mouse kidney sections.
    References
    • 1. Yasui, M. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 5808.
    • 2. Ikeda, M. et al. (2002) J. Biol. Chem. 277, 39873.
    • 3. Agre, P. et al. (2002) J. Physiol. 542, 3.
      • It has been long known that water must cross biological membranes by means other than simple diffusion. However, it was not until recently, with the discovery of the Aquaporin 1 water channel that this question was answered. The  importance of this discovery was underlined by the awarding, in 2003, the Nobel Prize in Chemistry to Peter Agre for the discovery of water channels. 

        Today, eleven mammalian proteins that belong to the Aquaporin family have been identified. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini.

        Aquaporin 6 (AQP6) appears to be exclusively expressed in the kidney, particularly in intracellular vesicles in collecting duct intercalated cells. Unlike the other aquaporins, AQP6 was found to allow permeation of anions following activation with acidic pH or Hg2+ ions. Increased expression of AQP6 was shown in models of chronic alkalosis and lithium-induced nephrogenic diabetes insipidus.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat AQP6. Anti-Aquaporin 6 Antibody (#AQP-006) can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP6 from mouse and rat samples.

    For research purposes only, not for human use